The effects of oral levothyroxine sodium application on serum copper concentration in rabbits

Bu çalışma oral %0.1’lik CuSO4 ile birlikte Levotiroksin Sodyum (T4) uygulamalarının serum Cu konsatrasyonu üzerindeki etkisinin tespit edilmesi için yapılmıştır. Kırk adet erkek Yeni Zelanda ırkı tavşan (6 aylık, ortalama ağırlıkları 2.29±0.06 kg) dört gruba ayrıldı. Bütün grupların içme sularına %0.1 CuSO4 (*5H2O) çalışmanın başından itibaren eklendi. Üç çalışma grubuna, kontrol grubu hariç 1.67, 3.33 ve 6.67 μg/kg-CA/gün dozunda T4 oral olarak uygulandı. Serum Cu ölçümleri Alev sistemli Atomic Absorbsiyon Spektrofotometre ile yapıldı. Takibenden günlerde serum Cu düzeyleri arttı ve uygulanan T4 miktarı ile serum Cu düzeyi arasında korelasyon tespit edildi (r=0.832, P

Anahtar Kelimeler:

bakır, kan kimyası, bakır sülfat, tiroksin, tavşan

Tavşanlarda oral levotiroksin sodyum uygulamasının Serum bakır konsantrasyonu üzerine etkileri

This study was carried out to determine the effect of oral Levothyroxine Sodium (T4) applications with oral CuSO4 (0.1%) on serum copper (Cu) concentration. Forty male New Zealand rabbits (6 month-old, weighing 2.29±0.06 kg) were allocated to four groups. All groups received 0.1% CuSO4 (*5H2O) (Copper (II) sulfate pentahydrate M102787) in drinking water with the beginning of the study. T4 was applied orally to three experimental groups at the doses of 1.67, 3.33 and 6.67 μg/kg-BW/day except control group. Serum Cu measurements were performed by Atomic Absorption Spectrometer equipped with Flame system. Serum Cu levels were increased on the following days and correlated with applied dose of T4 (r=0.832, P<0.001). The T4 affected serum Cu level with minimum r2=56.9% and P<0.001. The effect of time was found significant on serum Cu levels (P<0.001). There was also an interaction between time and groups on serum Cu levels (P<0.001). Consequently, T4 caused an increase in serum Cu level at second day and this increase continued on the following days..

Keywords:

copper, blood chemistry, copper sulfate, thyroxine, rabbits,

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