Ping LU, Wen-Tao MA, Ming PANG, Chen-Xiang ZUO, Ming-Jie LIU, Saad REHMAN, De-Kun CHEN, Qin-Lei FAN

Sağlıklı ve Mastitisli Keçi Sütünde αs1-kazeini Belirleyen Monoklonal Antikor Geliştirilmesi

Bu çalışma keçilerde mastitis sırasında kazein ekspresyon seviyesini değerlendirmeyi amaçlamıştır. Farelerde immünizasyon için birincil antijen olarak keçi kazeinleri kullanıldı. Keçi αs1-kazeine yüksek spesifite gösteren ve 5B olarak adlandırılan bir monoklonal antikor (mAb) geliştirildi. Sonuçlar mAb 5B’nin western blot ve ELISA’ya başarıyla uygulanabileceğini gösterdi. Ek olarak, geliştirilen mAb kullanılarak yapılan immünoflresan analizi, mastitisten etkilenen keçilerde süt αs1-kazein seviyesinin arttığını gösterdi. Sonuç olarak, bu çalışma ile mastitis gelişimi sırasında αs1-kazeinin ekspresyon seviyesini değerlendirmek için etkili bir faktör belirlenmiştir.

Development of a Monoclonal Antibody to Detect αs1-casein in the Milk of Healthy and Mastitis-Affcted Goats

This study aimed to evaluate the expression level of caseins during mastitis of goats. Whole goat caseins were used as primary antigensfor mouse immunization. A monoclonal antibody (mAb) named 5B with high specificity to goat αs1-casein was developed. Further resultsshowed that mAb 5B can successfully be applied to western blot and ELISA. In addition, immunoflorescence analysis using this mAbshowed increased milk αs1-casein level in mastitis-affcted goats. In conclusion, this study has established an effctive tool to evaluate theexpression level of αs1-casein during mastitis development.

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