LHRHa ([D-Ser$(But)^{6}, Pro^{9}$ -NEt-LHRHa) ve Domperidonun farklı kombinasyonlarda kullanımı ile ot sazanı (Ctenopharyngodon idellus valenciennes, 1844) anaçlarında yumurtlamanın uyarılması üzerine bir çalışma

Bu çalışmada ot sazanı anaçlarında yumurtlamayı uyarmak amacıyla, olgun anaçlara [D-Ser(But)6, Pro9-NEt]-LHRHa yüksek dozda tek başına (50 μg kg-1) ve değişen dozlarda (10, 20 μg kg-1) bir dopamin reseptör antagonisti olan Domperidonun (DOM) sabit dozu ile birlikte (10 mg kg-1) tek enjeksiyonda uygulanarak, lokal kuluçkahane şartları altında etkileri belirlendi. Elde edilen sonuçlar klasik hipofiz ekstraktı uygulaması (çift enjeksiyon) ile karşılaştırıldı (25ºC su sıcaklığında). Kontrol grubuna sadece fizyolojik tuzlu su verildi (%0.7 NaCl). Kontrol grubunda yumurtlama olmazken, LHRHa\'nın yüksek dozda tek başına verildiği grupta (50 μg kg-1) ve LHRHanın 10 μg kg-1 dozda 10 mg kg-1 DOM ile birlikte verildiği grupta anaçlarda yumurtlama oranı düşük gerçekleşti. LHRHa\'nın 20 μg kg-1 dozda 10 mg kg-1 DOM ile birlikte verildiği grupta ve hipofiz verilen grupta yumurtlama oranı yüksek olarak gerçekleşti. LHRHa+DOM verilen gruplarda enjeksiyondan sonra yumurta verme süresi, hipofiz ekstraktı verilen gruba göre yaklaşık 2 kat daha kısaldı (sırasıyla 9-10 saat, 17 saat). Yumurta veren tüm gruplar arasında anaçların yumurtlama indeksi ve yumurtaların döllenme oranları açısından farklılık gözlenmedi (P>0.05). Sonuç olarak, 20 μg kg-1 [D-Ser(But)6,Pro9-NEt]LHRHa+ 10 mg kg-1 DOM uygulaması ile ot sazanı anaçlarında yumurtlama, tek enjeksiyonda ve hipofize kıyasla daha kısa bir süre içinde ve yumurta kalitesinde düşmeye neden olmaksızın başarılı bir şekilde uyarıldı. Bu kombinasyonun uygulanması, ot sazanı yetiştiriciliğinde sağlıklı bir anaç ve kuluçkahane yönetimi açısından önemli faydalar sağlayabilir.

Anahtar Kelimeler:

antagonistler, balık, Ctenopharyngodon idella, su sıcaklığı, yumurta bırakmak, kuluçkahane, balık yumurtası, kültür balığı, döllenme, dopamin, biyokimyasal reseptörler

A study on spawning induction in grass carp (Ctenopharyngodon idellus valenciennes, 1844) by using different combinations of LHRHa ([D-Ser$(But)^{6}, Pro^{9}$-Net -LHRHa) and Domperidon

In this study, the effects of single administration of [D-Ser(But)6,Pro9-NEt]-LHRHa at high dose alone (50 μg kg-1), and at different doses (10, 20 μg kg-1) combined with constant dose of the dopamine receptor antagonist, Domperidon (10 mg kg-1), for induction of spawning in the grass carp broodstocks were determined under local hatchery conditions, and compared with classic Carp Pituitary Extract (CPE, double injection) application (Water temperature; 25ºC). Physiological salina (0.7 % NaCl) injected fish were used as control group. No spawning was observed in the control group. Spawning ratio was low in LHRHa at high dose alone treatment group (50 μg kg-1), and in 10 μg kg-1 dose of LHRHa at combined with 10 mg kg-1 dose of DOM treatment group. Spawning ratio was high in 20 μg kg-1 dose of LHRHa combined with 10 mg kg-1 dose of DOM treatment group, and in CPE treatment group. Latency period was shorter approximately two times in LHRHa+DOM treatment groups than CPE treatment groups (9-10 h, 17 h respectively). There was no differences between all spawning groups in respect of spawning index of broodstocks, and fertilisation rates of eggs (P>0.05). As a result, spawning was induced successfully in grass carp broodstocks by 20 μg kg-1 [D-Ser(But)6,Pro9-NEt]-LHRHa+10 mg kg-1 DOM treatment, in a single injection, in a shorter latency period and without decreasing in egg quality, comparing to CPE treatment. Application of this combination can be useful for healthy broodstock and hatchery management in grass carp culture.

Keywords:

antagonists, fishes, Ctenopharyngodon idella, water temperature, spawning, hatcheries, fish eggs, fish culture, fertilization, dopamine, biochemical receptors,

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