Mohammad Reza VALIZADE, Ali Asghar SADEGHI, Mohammad CHAMANI, Parvin SHAWRANG, Nasser KASHAN

The Effects of Increase in Threonine to Lysine Ratio on Performance, Blood Parameters and Humoral Immune Responses of Male Broiler Chickens Challenged with Salmonella

Bu çalışmanın amacı, etlik piliçlerde ideal lisin (Thr/Lys) oranının optimum performans, kan parametreleri ve immunite üzerine etkisini değerlendirmekti. 288 adet bir günlük erkek Ross 308 etlik civcivlere kontrol, treonin yönünden yetersiz ve yüksek ham selüloz diyetine treonin (türün treonin/lizin oranı gereksinimine eşit veya %25 daha fazla) eklendikten sonra verildi (üç uygulama ve her bir uygulama için sekiz tekrarlı tarzda). Bireylere 11. ve 21. günlükken sırasıyla bulaşıcı bursal hastalık virüsü ve bulaşıcı bronşit virüsü aşıları oral yolla ayrı ayrı uygulandı. Her uygulamadan 21 gün sonra, virüslere karşı kan antikor titreleri ölçüldü. 32 günlükken, her bir tedavi grubundan dört eşit sayıda piliç ağızdan Salmonella Paratyphi A (5 x 104 cfu/piliç) ile enfekte edildi. Bu çalışmanın sonucunda, Salmonella maruziyeti ölüm oranında artışa yol açarken, Thr/Lys oranındaki artış bu oranı azaltamadı. Thr/Lys oranındaki artış maruziyet olan ve olmayan grupların toplam yem alımı ve ağırlık kazançlarını azaltırken, maruziyet grubunun son periyottaki yem dönüşüm oranını artırdı. Yem tüketim maliyeti salmonellosis ile artarken, Thr/Lys oranındaki artış salmonellosis-kökenli ekonomik kaybı azaltamadı. Salmonellosis serum üre, ürik asit ve AST'yi artırıp serum glukoz ve kolesterolü azaltırken, Thr/Lys oranındaki artış ise trigliseridi baskılamadı. Thr/Lys oranındaki artış maruziyet olan ve olmayan gruplardaki humoral immun tepkiyi nispeten yükseltti. Bu bulgular, yüksek Thr/Lys oranının enfekte kuşlardaki üretim performansını artırdığını, ancak ekonomik yönden kazançlı bir tıbbi ajan olamadığını göstermektedir.

Salmonella'ya Maruz Kalan Erkek Broyler Piliçlerde ThreonineLysine Oranındaki Artışın Performans, Kan Parametreleri ve Humoral İmmun Tepki Üzerine Etkileri

The aim of this study was to assess the effect of threonine to lysine (Thr/Lys) ideal ratio for optimum performance, blood parameters and immunity of broiler chicks. Supplemental threonine (equal or 25% more than breed's threonine/lysine ratio requirement) was added to a control, threonine deficient and high crude fiber diet, then fed to 288 one-day-old male Ross 308 broiler chicks (three treatments and eight replications per treatment). On day 11 and 21 of age, infectious bursal disease virus and infectious bronchitis virus vaccines were orally administered individually, respectively. Then, 21 days after each administration, blood antibody titers against viruses were measured. On day 32 of age, four birds in each treatment group were infected orally with equal numbers of Salmonella Paratyphi A (5×104 cfu/bird). As a result of this study, challenging with Salmonella led to increase in mortality rate and increase in Thr/Lys ratio could not decrease it. Increase in Thr/Lys ratio decreased feed intake and weight gain of ensemble challenged and nonchallenged groups, however improved feed conversion ratio of challenged group in finisher period. Feed consumption cost increased by salmonellosis and increase in Thr/Lys ratio could not improve salmonellosis based economic loss. Salmonellosis increased serum urea, uric acid and AST and decreased serum glucose and cholesterol and increase in Thr/Lys ratio did not alleviate triglyceride. Increase in Thr/Lys ratio improved non-significantly humoral immune response in the challenged and non-challenged groups. These findings indicate that higher Thr/Lys ratio in infected birds improved production performance, however could not be an economical lucrative medicative agent.

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