ÖZGÜR ÇELEBİ, Salih OTLU, FATİH BÜYÜK, CELAL ŞAHİN ERMUTLU, ALİYE GÜLMEZ SAĞLAM, Elif ÇELİK, Doğan AKÇA, MİTAT ŞAHİN

The Isolation of Dichelobacter nodosus and Identification by PCR from Ovine Footrot in Kars District, Turkey

Bu araştırmada, Kars yöresinde footrotlu koyunlardan hastalığın spesifik etkenin izolasyonu, PCR ile identifikasyonu ve böylece hastalığın prevalansının saptanması amaçlanmıştır. Bu amaçla 10 farklı sürüye ait 8970 koyun klinik olarak incelenmiş 1532'sinde (%17.07) çeşitli nedenlere bağlı topallık görülmüştür. Bunlardan 247'si (%2.75) klinik açıdan footrot şüpheli olarak belirlendi. Footrot şüphesiyle anaerobik ortamda kültürel olarak değerlendirilen 247 örneğin 205 inde (%82.99) bakteriyel izolasyon gerçekleştirilmiş, izolatların yapılan Gram boyama ve mikroskobik incelenmeleri sonucu bunların 195'i (%95.12) Gram negative çomak morfolojisinde bakteriler olarak görülmüştür. Bu izolatlar Dichelobacter nodosus'a spesifik primer kullanılarak polymerase chain reaction (PCR) a tabi tutulmuş ve 153'ünde (%78.46) beklenen ağırlıkta (440 bp) amplikonlar saptanmıştır. Bu bulgular dikkate alındığında, Kars yöresindeki koyunlarda hastalığın prevalansının yüksek olduğu sonucuna varılmıştır.

Kars Yöresi Koyunlarında Piyeten Olgularından Dichelobacter nodosus İzolasyonu ve PCR İle İdentifikasyonu

In this study it was aimed to isolation and identification by PCR of specific agent from ovine footrot in Kars district and thus determination of prevalence of disease. To this end, 8,970 sheep belong to 10 different flocks were examined clinically, and in 1532 of these (17.07%) were found lameness for various reasons. Out of 247 (2.75%) of these cases were evaluated to be footrot suspect clinically. Bacteria were isolated in 205 (82.99%) of the 247 samples that were cultured in an anaerobic environment due to the suspicion of footrot. When Gram stains and microscopic investigation was carried out on these isolates, 195 of them (95.12%) were found to be Gram negative rod-type bacteria. These isolates were subjected by polymerase chain reaction (PCR) using Dichelobacter nodosus specific primer and amplicons (440bp) of expected weight in 153 (78.46%) of isolates were found. Considering of these findings, it was concluded that prevalence of disease is high in sheep in Kars district.

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