An Investigation on the Relationship between the AzoospermiaLike (DAZL) Gene mRNA Expression and the Infertility in Male Cattle-Yak

Bu çalışma erkek sığır-yak melezlerinde Azoospermia-Benzeri (DAZL) gen mRNA ekspresyon düzeyi ile infertilite arasındaki ilişkiyi araştırmak amacıyla yapılmıştır. Ekspresyon seviyeleri RT-PCR ile belirlendi. DAZL geninin spesifik olarak sığır, yak ve sığır-yak melezi testis dokularında eksprese edilmesi hücre siklusunun ilerlemesinde önemli rolü olduğunu teyit etmekteydi. Gerçek Zamanlı kantitatif PCR analizi sığır ve yak testis dokularında DAZL gen ekspresyon düzeyinin sığır-yak melezlerindekinden daha fazla olduğunu ve sığır-yak melezi ve yaklardaki seviyelerin anlamlı derecede sığırlardan farklı olduğunu göstermekteydi (P

Sığır-Yak Melezlerinde Azoospermia-Benzeri (DAZL) Gen mRNA Ekspresyon Düzeyi ile İnfertilite Arasındaki İlişki Üzerine Bir Araştırma

This study was conducted to study the relationship between the infertility of male cattle-yak and the expression level of DAZL gene mRNA. The expression profiles were obtained by RT-PCR. The DAZL gene was specifically expressed in cattle, yak and cattle-yak testis tissues,which confirmed its important role in the progression of cell cycle. Real-time quantitative PCR analysis indicated that the expression levels of DAZL gene in cattle and yak testis were higher than its in cattle-yak, also cattle-yak and yak were significantly differet than cattle, respectively (P<0.05). Therefore, the low expression level of DAZL gene might result in male cattle-yak infertility.

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Ding FH, Wei YP, Xu JT, Tong ZB, Ma ZJ, Chen SM, You La CR, Lou XL: The comparisons of the performance of yak, Pianniu and yellow cattle, and the determination of fiavour in the muscle. J Qinghai Univ, 26, 10, 2008.
Qu XG, Li QF, Liu ZS, Dong LY, Li XF, Hao CL, Xie Z: The study on the expression level of SYCP3 mRNA in Yak and Cattle-yak testis. Acta Vet Zootech Sinica, 39, 1132-1136, 2008.
Yen PH, Chai NN, Salido EC: The human autosomal gene DAZL A: testis specificity and a candidate for male infertility. Hum Mol Genet, 5, 2017, 1996.
Reynolds N, Cooke HJ: Role of the DAZ genes in male fertility. Reprod Biomed online, 10, 72-80, 2005. DOI: 10.1016/S1472-6483(10)60806-1
Yen PH: Putative biological functions of the DAZ family. Int J Androl, , 125-129, 2004.
Tschanter P, Kostova E, Luetjens C M, Cooper TG, Nieschlag E, Gromoll J: No association of the A260G and A386G DAZL single nucleotide polymorphisms with male infertility in a Caucasian population. Hum Reprod , 19, 2771-2776, 2004.
Xu EY, Moore FL, Pera RA: A gene family required for human germ cell development evolved from an ancient meiotic gene conserved in metazoans. Proc Natl, 98, 7414-7419, 2001.
Lin YM, Kuo PL, Lin YH, Eeng YN, Nan Lin JS: Messenger RNA transcripts of the meiotic regulation or BOULE in the testis of azoospermic men and their application in predicting the success of sperm retrieval. Hum Reprod, 20, 782-788, 2005. DOI: 10.1093/humrep/deh647
Tung JY, Luetjens CM, Wistuba J, Xu EY, Reijo Pera RA: Evolutionary comparison of the reproductive genes, DAZL and BOULE in primates with and without DAZ. Dev Genes Evol, 216, 158-168, 2006. DOI: 10.1007/ s00427-005-0039-2
Xu H, Li M, Gui J, Hong Y: Cloning and expression of medaka DAZL during embryogenesis and gametogenesis. Gene Expression Patterns, 7, 338, 2007.
Liu WS, Wang A, Uno Y, Galitz D, Beattie CW, Ponce de Leon FA: Genomic structure and transcript variants of the bovine DAZL gene. Cytogenet Genome Res, 116, 65-71, 2007.
Zhang QB, Li QF, Li JH, Li XF, Liu ZS, Song DW, Xie Z: b-DAZL: A novel gene in bovine spermatogenesis. Prog Nat Sci, 18, 1209-1218, 2008. DOI: 10.1016/j.pnsc.2008.02.012