The purpose of this study was to investigate the effects of using glauconite as a feed additive in broiler nutrition and its effect on fattening performance, intestinal histomorphology and biomechanical properties of bones in broilers. A total of 288 one-day-old male chicks were included in the study and they were randomly divided into 3 groups and these groups were divided into 8 subgroups. The trial continued for 35 days. The control group (C) was fed with basal ration while experimental groups were fed respectively with 1% glauconite (G1) and 2% glauconite (G2) added to the basal ration. The results showed that the use of different doses of glauconite in the rations did not statistically affect fattening performance. On the 21st and 35th day of the experiment, when histomorphology of ileum was examined, it was observed that villus height (VH), crypt depth (CD) and VH/CD ratio were not affected by glauconite addition. When histomorphology examination of jejeum was performed on the 35th day of the experiment, it was seen that the villus height was statistically affected. The effect of glauconite on biomechanical properties of femur and tibia bones was not statistically significant. As a result, the addition of glauconite in broiler rations did not affect the performance parameters, biomechanical properties of bones and histomorphology of the ileum, but adversely affected jejunum histomorphology.
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1. Venig SB, Serjantov VG, Chernova RK, Doronin SY, Selifonova EI, Zaharevich AM, Soldatenko EM: Glauconite of Saratov region, properties, composites based on it, the application. Butlerov Communications, 39 (8), 17, 2014.
2. Ibrahim SS, El Kammar AM, Guda AM, Boulos TR, Saleh A: Characterization and mineral beneficiation of Egyptian glauconite for possible industrial use. Particul Sci Technol, 37 (2): 141-150, 2019. DOI: 10.1080/02726351.2017.1376019
3. Avisar D, Primor O, Gozlan I, Mamane H: Sorption of sulfonamides and tetracyclines to montmorillonite clay. Water Air Soil Pollut, 209 (1-4): 439-450, 2010. DOI: 10.1007/s11270-009-0212-8
4. Stojiljkovic D, Josimov-Dundjerski J, Rajic M: Primena nekih mineralnih sirovina u savremenoj poljoprivredi. Letop Naucn Rad 26( 1): 86-92, 2002.
5. Wang J, Hu, J, Zhang S: Studies on the sorption of tetracycline onto clays and marine sediment from seawater. J Colloid Interface Sci 349 (2): 578-582, 2010. DOI: 10.1016/j.jcis.2010.04.081
6. Venig SB, Chernova RK, Doronin SY, Soldatenko EM, Selifonova EI, Sergantov VG, Zharevich AM, Shapoval OG: Synthesis, properties and antibacterial activity of the composites based on glauconite. BioNanoSci 7 (4): 659-665, 2017. DOI: 10.1007/s12668-017-0409-z
7. Subramaniam MD, Kim IH: Clays as dietary supplements for swine: A review. J Anim Sci Biotechnol 6:38, 2015. DOI: 10.1186/s40104-015-0037-9
8. Zhou Y, Xia M, Ye Y, Hu C: Antimicrobial ability of Cu2+-montmorillonite. Appl Clay Sci, 27 (3-4): 215-218, 2004.
9. Venig SB, Chernova RK, Sergantov VG, Selifonov AA, Shapoval OG,Nechaeva OV, Splyukhin VP, Zakharevich AM, Selifonova EI, Naumova GN, Scherbakova NN: Synthesis of glauconite composites and study of their antibacterial activity. J Biomed Photonics Eng, 2 (4): 040303-1-040303-5, 2016. DOI: 10.18287/JBPE16.02.04030
10. Baurhoo B, Ferket PR, Zhao X: Effects of diets containing different concentrations of mannanoligosaccharide or antibiotics on growth performance, intestinal development, cecal and litter microbial populations, and carcass parameters of broilers. Poult Sci, 88 (11): 2262–2272, 2009. DOI: 10.3382/ ps.2008-00562
11. Su JQ, Chu Xl, Lıu WQ, Fu DB, Wang L, Wang CY, Shen HQ, Weı XB: Clinical application and mechanism of Chinese herbal medicine against chicken colibacillosis. Hubei Agric Sci, 20, 7-8, 2009.
12. NRC: Nutrient Requirements of Poultry. Washington, DC: National Academy Press. 1994.
13. AOAC: Official Methods of Analysis of AOAC International. Gaithersburg, MD, 2000.
14. Culling CFA, Allison RT, Bar WT: Cellular Pathology Technique. Butterworth and Co. Ltd., London. 1985.
15. Sakamoto K, Hirose H, Onizuka A, Hayashi M, Futamura N, Kawamura Y, Ezaki T: Quantitative study of changes in intestinal morphology and mucus gel on total parenteral nutrition in rats. J Surg Res, 94 (2): 99-106, 2000
16. Gurgul S, Erdal N, Yilmaz SN, Yildiz Ankarali A: Deterioration of bone quality by long-term magnetic field with extremely low frequency in rats. Bone, 42 (1): 74-80, 20008. DOI: 10.1016/j.bone.2007.08.040
17. SPSS: Statistical Packages for the Social Sciences, 20th edn., IBM Inc, Chicago, USA, 2011.
18. Ouachem D, Kaboul N, Meredef A, Abdessemed F, Gaid ZA : Effects of clay on performance, moisture of droppings and health status of poultry: An overview Worlds Poult Sci J, 71 (1):184-189, 2015. DOI: 10.1017/ S004393391500015X
19. Ouhida I, Perez JF, Gasa J, Puchal F: Enzymes (β-glucanase and arabinoxylanase) and/or sepiolite supplementation and the nutritive value of maize-barley wheat based diets for broiler chickens. Br Poult Sci, 41, 617-624, 2000. DOI: 10.1080/713654974
20. Varol Avcilar Ö, Kocakaya A, Onbasilar EE, Pirpanahi M: Influence of sepiolite additions to different litter materials on performance and some welfare parameters of broilers and litter characteristics. Poult Sci, 97 (9): 3085- 3091, 2018. DOI: 10.3382/ps/pey185
21. Karamanlis X, Fortomaris P, Arsenos G, Dosis I, Papaioannou D, Batzios C, Kamarianos A: The effect of a natural zeolite (clinoptilolite) on the performance of broiler chickens and the quality of their litter. Asian-Australas J Anim Sci, 21 (11): 1642-1650, 2008. DOI: 10.5713/ajas.2008.70652
22. Yalcın S, Yalcin S, Gebes ES, Sahin A, Duyum HM, Escribano Fi Ceylan A: Sepiolite as a feed supplement for broilers. Appl Clay Sci, 148, 95-102, 2017. DOI: 10.1016/j.clay.2017a.08.007
23. Donnic IM, Neverova OP, Gorelik OV: Use of zeolites for increase feeding qualities of animals. Ural Agrarian Bulletin, 9, 41-47, 2015.
24. Pasha TN, Mahmood A, Khattak FM, Jabbar MA, Khan AD: The effect of feed supplemented with different sodium bentonite treatments on broiler performance. Turk J Vet Anim Sci, 32 (4): 245-248, 2008.
25. Safari MH, Shams SM, Shargh MS, Amini A, Tatar A: Effects of different levels of natural glauconite and zeolite on performance, tibia bone characteristics and blood parameters of broiler chicken. Anim Sci J, 2015, 167- 178. 2015,
26. Yalcin S, Onbasilar I, Gebes ES, Ramay MS, Gunturkun OB: Effects of sepiolite usage in the manufacturing of pellet concentrate feeds for dairy cattle and fattening cattle on some production parameters and pellet quality characteristics. Lalahan Hay Araşt Enst Derg, 57 (1): 39-43, 2017.
27. Katouli MS, Boldaji F, Dastar B, Hassani S: Effect of different levels of kaolin, bentonite and zeolite on broilers performance. J Biol Sci, 10 (1):5 8-62, 2010. DOI: 10.3923/jbs.2010.58.62
28. Calik A, Ergün A: Effect of lactulose supplementation on growth performance, intestinal histomorphology, cecal microbial population, and short-chain fatty acid composition of broiler chickens. Poult Sci, 94 (9): 2173- 2182, 2015. DOI: 10.3382/ps/pev182
29. Wu QJ, Wang LC, Zhou YM, Zhang JF, Wang T: Effects of clinoptilolite and modified clinoptilolite on the growth performance, intestinal microflora, and gut parameters of broilers. Poult Sci, 92 (3): 684-692, 2013. DOI: 10.3382/ ps.2012-02308
30. Tatar A, Boldaji F, Dastar B, Yaghobfar A: Comparison of different levels of zeolite on serum characteristics, gut pH, apparent digestibility of crude protein and performance of broiler chickens. International Zeolite Conference, Tehran, Iran, 2008.
31. Xia MS, Hu CH, Xu ZR: Effects of copper-bearing montmorillonite on growth performance, digestive enzyme activities, and intestinal microflora and morphology of male broilers. Poult Sci, 83 (11): 1868-1875, 2004. DOI: 10.1093/ps/83.11.1868
32. Shim MY, Karnuah AB, Mitchell AD, Anthony NB, Pesti GM, Aggrey SE: The effects of growth rate on leg morphology and tibia breaking strength, mineral density, mineral content, and bone ash in broilers. Poult Sci, 91 (8): 1790-1795, 2012. DOI: 10.3382/ps.2011-01968
33. Ferket PR, Oviedo-Rondón EO, Mente L, Bohórquez DV, Santos JR AA, Grimes JL, Richards JD, Dibner JJ, Felts V: Organic trace minerals and 25-hydroxycholecalciferol affect performance characteristics, leg abnormalities, and biomechanical properties of leg bones of turkeys. Poult Sci, 88 (1): 118– 131, 2009. DOI: 10.3382/ps.2008-00200
34. Arslan Kaya H, Macit M: Yumurtlamanın son dönemindeki yumurtacı tavukların rasyonlarına bor (ortoborik asit) ilavesinin yumurta kabuk kalitesi ve tibia biyomekaniği parametreleri ile serum, kabuk ve tibia mineral konsantrasyonları üzerine etkisi. Atatürk Üniv Vet Bil Derg, 13 (1): 42-53, 2018. DOI: 10.17094/ataunivbd.315617
35. Çalik A, Yalçin S, Küçükersan S, Saçakli P, Yildiz G, Ramay MS, Ahlat O, Erbay Elibol FK, Taban S: Effects of calcium soaps of animal fats on performance, abdominal fat fatty acid composition, bone biomechanical properties, and tibia mineral concentration of broilers. Kafkas Univ Vet Fak Derg, 25 (1): 61-70, 2019. DOI: 10.9775/kvfd.2018.20329