Estrous synchronization with used CIDR-G Devices in ewes during non-breeding season
Bu çalışmanın amacı, koyunlarda daha önce kullanılmış olan CIDR-G’nin östrus senkronizasyonu amacıyla tekrar kullanılabilirliğini araştırmaktı. Çalışma, anöstrus döneminde 58 adet koyunda yapıldı. Koyunlar dört ayrı gruba ayrıldı. Grup I; CIDR-G 12 gün boyunca vaginada bırakıldı (CIDR Grubu). Grup II; daha önce kullanılmış CIDR-G 12 gün boyunca vaginada bırakıldı (U-CIDR Grubu). İntravaginal alet uygulamalarını takiben aletlerin çıkarıldığı gün Grup I ve II’deki koyunlara 500 IU gebe kısrak serum gonadotropini (PMSG), i.m. yolla uygulandı. Grup III’teki koyunlara sadece 500 IU PMSG uygulandı. Kontrol grubu olarak oluşturulan Grup IV’teki koyunlara %0.9 NaCl’den 3 ml uygulandı. Kontrol grubundaki koyunların hiçbirinde östrus gözlenmedi. Östrus görülme oranları CIDR, U-CIDR ve PMSG grupları için sırasıyla %86.67 (13/15), %66.67 (10/15) ve %7.69 (1/13) olarak belirlendi. Gebelik oranları, CIDR grubunda %53.33 (8/15), U-CIDR grubunda %60 (9/15) ve PMSG grubunda %7.69 (1/13) olarak saptandı. PMSG uygulamasından sonra östrus görülene kadar geçen süreye bakıldığında CIDR, U-CIDR ve PMSG grupları için sırasıyla 42.6±1.01, 40.13±1.35 ve 74 saat olarak kaydedildi. Uygulama süresi boyunca ortalama progesteron seviyesi CIDR grubunda 3.28±0.28 ve U-CIDR grubunda 2.62±0.14 ng/ml düzeyinde oldu. Araştırma sonucunda, daha önce koyunlarda 12 gün süreyle kullanılmış olan CIDR-G’nin koyunlarda anöstrus mevsiminde fertil östruslar uyarılabildiği görüldü.
Koyunlarda anöstrus mevsiminde kullanılmış CIDR-G uygulamasıyla östrusun uyarılması
The objective of this study was to investigate the efficacy of used controlled internal drug release devices (CIDR-G) on estrous synchronization in ewes. This investigation was conducted in 58 fat-tailed ewes during the non-breeding season. Ewes were divided in to four groups. In Group I, CIDR-G devices were inserted to vagina for 12 d (CIDR group). In Group II, previously used CIDR-G devices were inserted into the vagina for 12 d (U-CIDR). After the intravaginal devices had been removed 500 IU equine chorionic gonadotropin (eCG) was injected intramuscularly (i.m.) into ewes of the CIDR and U-CIDR groups. Ewes in Group III (eCG group), 500 IU eCG was injected im. Ewes in Group IV, served as control and received an injection of 3 mL 0.9% NaCl (C Group). None of the control ewes expressed estrus. It was found that, 13/15 (86.67%), 10/15 (66.67%) and 1/13 (7.69%) ewes expressed estrus in CIDR, U-CIDR and eCG groups, respectively. The pregnancy rates in the CIDR, U-CIDR and eCG groups were 8/15 (53.33%), 9/15 (60%) and 1/13 (7.69%) at first breeding, respectively. The interval from eCG application to onset of estrus was 42.6±1.01, 40.13±1.35 and 74 h in the CIDR, U-CIDR and eCG groups, respectively. Average progesterone (P4) levels during P4 treatment were 3.28±0.28 and 2.62±0.14 ng/ml in the CIDR and U-CIDR groups, respectively. It is concluded that, CIDR-G that have been previously used for 12 d could be used for the synchroniation of estrus in ewes during non-breeding season.
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