Estrous synchronization with used CIDR-G Devices in ewes during non-breeding season

Bu çalışmanın amacı, koyunlarda daha önce kullanılmış olan CIDR-G’nin östrus senkronizasyonu amacıyla tekrar kullanılabilirliğini araştırmaktı. Çalışma, anöstrus döneminde 58 adet koyunda yapıldı. Koyunlar dört ayrı gruba ayrıldı. Grup I; CIDR-G 12 gün boyunca vaginada bırakıldı (CIDR Grubu). Grup II; daha önce kullanılmış CIDR-G 12 gün boyunca vaginada bırakıldı (U-CIDR Grubu). İntravaginal alet uygulamalarını takiben aletlerin çıkarıldığı gün Grup I ve II’deki koyunlara 500 IU gebe kısrak serum gonadotropini (PMSG), i.m. yolla uygulandı. Grup III’teki koyunlara sadece 500 IU PMSG uygulandı. Kontrol grubu olarak oluşturulan Grup IV’teki koyunlara %0.9 NaCl’den 3 ml uygulandı. Kontrol grubundaki koyunların hiçbirinde östrus gözlenmedi. Östrus görülme oranları CIDR, U-CIDR ve PMSG grupları için sırasıyla %86.67 (13/15), %66.67 (10/15) ve %7.69 (1/13) olarak belirlendi. Gebelik oranları, CIDR grubunda %53.33 (8/15), U-CIDR grubunda %60 (9/15) ve PMSG grubunda %7.69 (1/13) olarak saptandı. PMSG uygulamasından sonra östrus görülene kadar geçen süreye bakıldığında CIDR, U-CIDR ve PMSG grupları için sırasıyla 42.6±1.01, 40.13±1.35 ve 74 saat olarak kaydedildi. Uygulama süresi boyunca ortalama progesteron seviyesi CIDR grubunda 3.28±0.28 ve U-CIDR grubunda 2.62±0.14 ng/ml düzeyinde oldu. Araştırma sonucunda, daha önce koyunlarda 12 gün süreyle kullanılmış olan CIDR-G’nin koyunlarda anöstrus mevsiminde fertil östruslar uyarılabildiği görüldü.

Koyunlarda anöstrus mevsiminde kullanılmış CIDR-G uygulamasıyla östrusun uyarılması

The objective of this study was to investigate the efficacy of used controlled internal drug release devices (CIDR-G) on estrous synchronization in ewes. This investigation was conducted in 58 fat-tailed ewes during the non-breeding season. Ewes were divided in to four groups. In Group I, CIDR-G devices were inserted to vagina for 12 d (CIDR group). In Group II, previously used CIDR-G devices were inserted into the vagina for 12 d (U-CIDR). After the intravaginal devices had been removed 500 IU equine chorionic gonadotropin (eCG) was injected intramuscularly (i.m.) into ewes of the CIDR and U-CIDR groups. Ewes in Group III (eCG group), 500 IU eCG was injected im. Ewes in Group IV, served as control and received an injection of 3 mL 0.9% NaCl (C Group). None of the control ewes expressed estrus. It was found that, 13/15 (86.67%), 10/15 (66.67%) and 1/13 (7.69%) ewes expressed estrus in CIDR, U-CIDR and eCG groups, respectively. The pregnancy rates in the CIDR, U-CIDR and eCG groups were 8/15 (53.33%), 9/15 (60%) and 1/13 (7.69%) at first breeding, respectively. The interval from eCG application to onset of estrus was 42.6±1.01, 40.13±1.35 and 74 h in the CIDR, U-CIDR and eCG groups, respectively. Average progesterone (P4) levels during P4 treatment were 3.28±0.28 and 2.62±0.14 ng/ml in the CIDR and U-CIDR groups, respectively. It is concluded that, CIDR-G that have been previously used for 12 d could be used for the synchroniation of estrus in ewes during non-breeding season.

___

  • 1. Arthur HG, Noakes DE, Pearson H: The oestrous cycle and its control. Veterinary Reproduction and Obstetrics, 6th ed., W.B. Saunders Co., Philadelphia. 1989.
  • 2.Baştan A, Küplülü Ş: The effect of progestagen and melatonin on reproductive performance in Akkaraman ewes. Ankara Üniv Vet Fak Derg, 42, 263-70, 1995.
  • 3.Wildeus S: Current concepts in synchronization of estrus: sheep and goats. Proceedings of the American Society of Animal Science. 1-14, 1999.
  • 4.Schoenian S: Reproduction in the ewe. http://www. sheep101.info/201/ewerepro.html, Accessed: 2005.
  • 5.Simonetti L, Blanco MR, Gardon JC: Estrus synchronization in ewes treated with sponges impregnated with different doses of medroxyprogesterone acetate. Small Rumin Res, 38, 243247, 2000.
  • 6.Crosby TF, Boland MP, Gordon I: Effect of progestagen treatments on the incidence of oestrus and pregnancy rates in ewes. Anim Reprod Sci, 24, 109-18, 1991.
  • 7.Godfrey RW, Gray ML, Collins JR: A comparison of two methods of oestrus synchronization of hair sheep in the trophics. Anim Reprod Sci, 47, 99-106, 1997.
  • 8.Colazo MG, KastelicJP, Whittaker PR, Gavaga QA, Wilde R, Mapletoft RJ: Fertility in beef cattle given a new or previously used CIDR insert and estradiol, with or without progesterone. Anim Reprod Sci, 81, 25-34, 2004.
  • 9.Zuluaga JF, Williams GL: High-pressure steam sterilization of previously used CIDR inserts enhances the magnitude of the acute increase in circulating progesterone after insertion in cows. Anim Reprod Sci, 107, 30-35, 2008.
  • 10.Jabbour HN, Evans G: Ovarian and endocrine responses of Merino ewes to treatment with PMSG and/or FSH-P. Anim Reprod Sci, 26, 93-106, 1991.
  • 11.Prakash BS, Meyer HHD, Schallenberger E, Van De Weil DFM: Development of sensitive Enzymeimmunoassay (EIA) for progesterone determination in unextracted bovine plasma using the second antibody technique. J Steriod Biochem, 28, 623-627, 1987.
  • 12.Ungerfeld R. Rubianes E: Estrous response to ram effect in Corriedale ewes primed with medroxyprogesterone during the breeding season. Small Rumin Res, 32, 89-91, 1999.
  • 13.Stenbak T K, Grazul-Bilska AT, Berginski HR, Bilski JJ, Erickson AS. Kirsch JD, Kraft KC, Navanukraw C, Toutges MC, Reynolds LP, Redmer DA: Ovulation rate in ewes synchronized with Syncro-Mate-B (SMB) and follicle stimulating hormone. Small Rumin Res, 48, 1-8, 2003.
  • 14.Greyling JPC, Brink WCJ: Synchronization of oestrus in sheep: The use of controlled internal drug release (CIDR) dispensers. S Afr J Anim Sci, 10, 35-41, 1987.
  • 15.VanCleff J, Karsch FJ, Padmanabhan V: Characterization of endocrine events during the periestrous period in sheep after estrous synchronization with controlled internal drug release (CIDR) device. Dom Anim Endocrinol, 15, 23-24, 1998.
  • 16.Godfrey RW, CollinsJR, Hensley EL, Wheaton JE: Estrus synchronization and artificial insemination of hair sheep ewes in the tropics. Theriogenology, 51, 985-997, 1999.
  • 17.Gungor O, Cenesiz M, Pancarci SM, Yildiz S, Kaya M, Kacar C, Ozyurtlu N, Gurbulak K: Effects of different intravaginal progesterone releasing devices on estrous synchronization and LH surge in fat-tailed ewes during non-breeding season. Med Weter, 63, 1316-19, 2007.
  • 18.Leyva V, Buckrell BC, Walton JS: Follicular activity and ovulation reglated by exogenous progstagen and PMSG in anestrous ewes. Theriogenology, 50, 377-93, 1998.
  • 19.McNeilly AS, Fraser HM: Effect of gonadotrophin-releasing hormone agonist-induced suppression of LH and FSH on follicle growth and corpus luteum function in the ewe. J Endocrinol, 115, 273-282, 1987.
  • 20.Viñoles C, Fosberg M, Banchero G, Rubianes E: Effect of long-term and short-term progestagen treatment on follicular development and pregnancy rate in cyclic ewes. Theriogenology, 55, 993-1004, 2001.
Kafkas Üniversitesi Veteriner Fakültesi Dergisi-Cover
  • ISSN: 1300-6045
  • Yayın Aralığı: Yılda 6 Sayı
  • Başlangıç: 1995
  • Yayıncı: Kafkas Üniv. Veteriner Fak.
Sayıdaki Diğer Makaleler

Pathologic changes and use of immunohistochemical methods in naturally occuring avian encephalomyelitis of the chicks

Nihat TOPLU, Günay ALÇIGIR, Recai TUNCA

Determination of Clostridium botulinum toxins in dairy cows with abomasal displacement

Sırrı AVKİ, Hülya TÜRÜTOĞLU, Rüçhan ALP, KÜRŞAD YİĞİTARSLAN, M. Doğa TEMİZSOYLU

Geçiş dönemi başındaki akkaraman melezi koyunlarda farklı dozda flourogestene acetate, norgestomet ve PGF2α ile senkronize östrüslerin uyarılması

MEHMET BOZKURT ATAMAN, MEHMET AKÖZ, MURAT FINDIK, Erdal SABAN

Radyo frekans sinyalinin rat spinal ganglion hücrelerinde kalsiyum bağımlı klor kanallarına etkisi

ALİRIZA ERDOĞAN, Erkan İPEKÇİOĞLU

Deneysel olarak fibrosarkoma oluşturulan ratların serumlarında İnterlökin 6(IL-6) ve Tümör nekrosis faktör-∝ (TNF-∝) düzeylerinin belirlenmesi

Dilek SACU, Ayşegül BİLDİK

Bir Montafon inekte sekum dilatasyonu ve dislokasyonu

İSMAİL ALKAN, LOĞMAN ASLAN, ABDULLAH KARASU, Yakup AKGÜL, Erkan DÜZ, Eda YAVUZ

The efficacy of carboxymetylcellulose for prevention adhesion formation after thyroid region surgery

Yusuf GÜNERHAN, Kasım ÇAĞLAYAN, Aziz SÜMER, Neşet KÖKSAL, Ediz ALTINLI, Ender ONUR, Pembegül GÜNEŞ, Gulistan GUMRUKCU

Keçilerde akut nitrat zehirlenmesi

MUSTAFA İSSİ, Yusuf GÜL, Selçuk İLHAN, ONUR BAŞBUĞ, BURCU GÜL BAYKALIR

The effect of basic fibroblast Growth factor (bFGF) on in vitro embryonic Growth, heart and neural tube development in rat

HARUN ÜLGER, SAİM ÖZDAMAR, ERDOĞAN UNUR, Margaret PRATTEN

In vitro effects of culture medium and serum on germ cells in testis and epididymis of male wistar rats

Enis CEZAYİRLİ, M. İbrahim TUĞLU, KAMİL VURAL