OKTAY TOMAR, GÖKHAN AKARCA, Mehmet BEYKAYA, ABDULLAH ÇAĞLAR

Some Characteristics of Erzincan Tulum Cheese Produced Using Different Probiotic Cultures and Packaging Material

In this study, Erzincan tulum cheeses produced using pasteurized sheep milk and probiotic bacterial cultures were filled in different packaging materials (skin bag, intestine, and appendix) and stored for 90 days. Subsequently, the chemical, microbiological and quality criteria of the samples were examined on the 2nd, 30th, 60th and 90th days of the storage period. Although the acidity values of cheese samples increased during the storage period, the pH and water activity values decreased. Similarly, TAMB, TAPB, yeast/mold, lactic acid bacteria, lipolytic bacteria, proteolytic bacteria, Lactococcus spp. and Pseudomonas spp. counts increased; however, the total coliform and Enterobacteriaceae counts decreased. The probiotic culture counts added in cheese production also decreased during storage. Moreover, no Escherichia coli, Staphylococcus aureus, Salmonella spp., Listeria spp. and Brucella spp. development was determined in the samples.

Farklı Probiyotik Kültür ve Ambalaj Malzemesiyle Üretilen Erzincan Tulum Peynirlerinin Bazı Özelliklerinin İncelenmesi

Bu çalışmada, pastörize koyun sütleri kullanılarak ve probiyotik bakteri kültürleri ilave edilerek üretilen Erzincan tulum peynirleri farklı ambalaj materyallerine doldurularak (tulum, ince bağırsak, kör bağırsak, 90 gün süre ile depolanmıştır. Depolamanın 2., 30., 60., ve 90. günlerinde peynirlerin bazı kimyasal ve mikrobiyolojik, kalite kriterleri incelenmiştir. Depolama süresi boyunca peynir örneklerinin % asitlik değerleri artış göstermesine karşın, pH ve aw değerlerinde azalma olduğu belirlenmiştir. Benzer şekilde depolama süresince TAMB, TAPB, maya/küf, laktik asit, lipolitik bakteri, proteolitik bakteri sayıları, Lactococcus spp. ve Pseudomonas spp. cinsi bakteri sayılarında artış tespit edilmesine karşın, Toplam koliform ve Enterobacteriaceae sayılarında ise azalma olduğu belirlenmiştir. Peynir üretiminde ilave edilen probiyotik kültür sayıları da yine depolama süresince azalmıştır. Ayrıca peynirlere yapılan Escherichia coli, Staphylococcus aureus, Salmonella spp, Listeria Spp. Brucella spp. analizleri sonucunda herhangi bir gelişme tespit edilememiştir.

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Morul F, İşleyici Ö: Chemical and microbiological properties of Divle tulum cheese. Y Y U Vet Fak Derg, 23 (2): 71-76, 2012.

Çakır Y: The influence of some quality characteristics of Erzincan tulum cheese on (Nigella sativa L.) Nigella. Msc Thesis, Atatürk University, Institute of Natural and Applied Science, Turkey, 2012.

Andiç S, Tunçtürk Y, Gençcelep H: Effect of freezing storage and vacuum packaging on lipolysis and organic acid exchange in motal cheese. Food, 35 (6): 423-430, 2010.

ISO: International Standard Organization, 6888-1 Horizontal Method for the Enumeration of Coagulase- positive Staphylococci Technique using Baird Parker Agar Medium, 1999.

ISO: International Standard Organization, 4832 General Guidance fort the Enumeration of Coliforms Colony Count Technique, 1991.

36. ISO: International Standard Organization, ISO 21528-2:2004 Microbiology of food and animal feeding stuffs Horizontal methods for the detection and enumeration of Enterobacteriaceae. 2004.

ISO: International Standard Organization, 16649-3:2015 Microbiology of the food chain- Horizontal method for the enumeration of betaglucuronidase-positive Escherichia coli - Part 3: Detection and most probable number technique using 5-bromo-4-chloro-3-indolyl-ß-Dglucuronide, 2015.

ISO: International Standard Organization, 16649-2:2001 Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli - Part 2: Colony-count technique at 44 degrees C using 5-bromo-4-chloro-3-indolyl beta-Dglucuronide, 2001.

ISO: International Standard Organization, 16649-1:2001 Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli - Part 1: Colony-count technique at 44 degrees C using membranes and 5-bromo-4-chloro-3- indolyl beta-D-glucuronide, 2001.

ISO: International Standard Organization, 13720:2010 specifies a method for the enumeration of presumptive Pseudomonas spp. present in meat and meat products, including poultry, 2010.

ISO: International Standard Organization, 6579-1:2017 Horizontal method for the detection, enumeration and serotyping of Salmonella, 2017.

ISO: International Standard Organization, 4833-1:2013 Microbiology of the food chain - Horizontal method for the enumeration of microorganisms - Part 1: Colony count at 30 degrees C by the pour plate technique, 2013.

ISO: International Standard Organization, 4833-2:2013 Horizontal method for the enumeration of microorganisms - Part 2: Colony count at 30 degrees C by the surface plating technique, 2013.

ISO: International Standard Organization, 6579-1:2017 Horizontal method for the detection, enumeration and serotyping of Salmonella, 2017.

Flowers RS, D’aust JY, Andrews WH, Bailey JS: Salmonella. In, Vanderzant C, Spilttstoesser DF (Eds): Compendium of the Methods for the Microbiological Examinations of Foods. 371-422, American Public Health Association, 1992.

ISO: International Standard Organization. 11290-2:2017 Microbiology of the food chain, Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. - Part 2: Enumeration method, 2017.

ISO: International Standard Organization, 11290-1:2017 Microbiology of the food chain, Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. - Part 1: Detection method, 2017.

Halkman K: Gıda mikrobiyolojisi uygulamaları. Başak Matbaacılık Ltd. Şti, Ankara, Turkey, 2005.

TSE: Turkish Standard Institute, TS 6235 EN ISO 6887-1. Microbiology of Food and Animal Feed. General Rules of The Initial Suspension and Decimal Dilutions of Test Samples Preparation, 2001.

AOAC: Official Methods of Analysis. Official Method 937.05Lactic Acid in Milk and Milk Products. Assoction of Official Analytical Chemists, Washington, 2000.

Dikici A: During the production and development of Şavak tulum cheese investigation of life and acid adaptation capability of Escherichia coli O157: H7, Listeria monocytogenes and Salmonella. PhD Thesis, Fırat University, Institute of Health Sciences, Turkey, 2008.

Dave RI, Shah NP: Viability of yoghurt and probiotic bacteria in yoghurt made from commercial starter cultures. Int Dairy J, 7 (1): 31-41, 1997. DOI: 10.1016/S0958-6946(96)00046-5

Gibson GR, Fuller R: The role of probiotics and prebiotics in the functional food concept. In, Sadler MJ, Saltmarsh M (Eds): Functional Foods: The Consumer, The Products and the Evidence. 3-14, Royal Society of Chemistry, Cambridge, 1998.

Salminen S: Probiotics: Scientific support for use. Food Technol, 53 (11): 66-77, 1999.

Çakır İ: Determination of some probiotic properties in Lactobacillus and Bifidobacter. PhD Thesis, Ankara University, Institute of Natural and Applied Science, Turkey, 2003.

Çağlar A, Bor Y, Tomar O, Beykaya M, Gök V: Mechanical and microbiological properties of natural casings using in meat products. Kafkas Univ Vet Fak Derg, 24 (3): 327-334, 2018. DOI: 10.9775/ kvfd.2017.18885

Akarca G, Tomar O, Gök V: Effect of different packaging methods on the quality of stuffed and sliced mozzarella cheese during storage. J Food Process Preserv, 39 (6): 2912-2918, 2015. DOI: 10.1111/jfpp.12542

Çakmakçı S: Development of traditional method production and production technology of Erzincan tulum (Savak) cheese. V. Milk and Dairy Products Symposium, 21-22 May, Tekirdağ, MPM Publications No: 621, 117-125s, Ankara, Turkey, 1998.

Şengül M, Türkoğlu H, Çakmakçı S, Çon AH: Effects of casing materials and ripening period on some microbiological properties of tulum cheese. Pak J Biol Sci, 4 (7): 854-857, 2001. DOI: 10.3923/pjbs. 2001.854.857

Şengül M, Çakmakçı S: Microbial quality of tulum cheeses marketed in eastern part of Turkey. International Dairy Symposium, 24-28 May, 96- 99, Isparta, Turkey, 2004.

Kurt A, Çakmakçı S, Çağlar A, Akyüz N: A study on the structure, sensory, physical and chemical properties of Erzincan tulum (Savak) cheese. Food, 16 (5): 295-302, 1991.

Öner Z, Şimşek B, Sağdıç O: Determination of some properties of Turkish tulum cheese. Milchwissenschaft, 58 (3-4): 152-154, 2003.

Yilmaz G, Ayar A, Akin N: The effect of microbial lipase on the lipolysis during the ripening of tulum cheese. J Food Eng, 69 (3): 269-274, 2005. DOI: 10.1016/j.jfoodeng.2004.08.017

Şengül M: Starter culture characteristics of some lactic acid bacteria strains isolated from tulum cheese and determination of some properties of cheese. PhD Thesis, Atatürk University, Institute of Natural and Applied Science, Turkey, 2001.

Çakmakçı S, Dağdemir E, Hayaloğlu AA, Gürses M, Gündoğdu E: Influence of ripening container on the lactic acid bacteria population in tulum cheese. World J Microbiol Biotechnol, 24, 293-299, 2008. DOI: 10.1007/s11274-007-9470-z

6. Çakmakçı S, Çakır Y: Erzincan tulum (Savak) cheese and our similar cheese. 3. Traditional Foods Symposium, 10-12 May, 218, Konya, Turkey, 2012.

5. Karaibrahimoğlu Y, Üçüncü M: Determination of process and product parameters of Erzincan tulum cheese. E U Muhendislik Fak Derg, 6 (2): 79- 97, 1988

Demir P, Öksüztepe G, İncili KG, İlhak Oİ: Vakum paketli Şavak tulum peynirlerinde potasyum sorbatın kullanımı. Kafkas Univ Vet Fak Derg, 23 (1): 23-30, 2017. DOI: 10.9775/kvfd.2016.15707

Beresford TP, Fitzsimons NA, Brennan NL, Cogan TM: Recent advances in cheese microbiology. Int Dairy J, 11 (4-7): 259-274, 2001. DOI: 10.1016/S0958-6946(01)00056-5

Çetinkaya A: Yöresel Peynirlerimiz. 212, Academic Book Production, Kars, Turkey 2005.

Akın N: Temel Peynir Bilimi-I. Damla Ofset, Konya, Turkey, 2010.

Öksüztepe G, Patır B, Çalıcıoğlu M: Identification and distribution of lactic acid bacteria during the ripening of Şavak tulum cheese. Turk J Vet Anim Sci, 29 (3): 873-879, 2005.

Çetin B, Gürses M, Şengül M: Effect of relative humidity change on some microbiological properties of tulum cheese. Turkey 9. Food Congress, 24-26 May, Bolu, Turkey, 2006.

Kara R, Akkaya L: Afyon tulum peynirinin mikrobiyolojik ve fizikokimyasal özellikleri ile laktik asit bakteri dağılımlarının belirlenmesi. AKU J Sci Eng, 15 (1): 1-6, 2015. DOI: 10.5578/fmbd.8717

Hayaloğlu AA, Çakmakçı S, Brenchany EG, Deegan KC, McSweeney PLH: Microbiology, biochemistry and volatile composition of tulum cheese ripened in goat’s skin or plastic bags. J Dairy Sci, 90 (3): 1102-1121, 2007. DOI: 10.3168/jds.S0022-0302(07)71597-7

Güven M, Konar A: İnek sütlerinden üretilen ve farklı ambalajlarda olgunlaştırılan tulum peynirlerinin mikrobiyolojik özellikleri. Gıda, 19 (3): 179-185, 1994.

Çağlar A: Çiğ sütten üretilen ve farklı ambalajlama materyallerinde olgunlaştırılan Erzincan tulum peynirlerinin mikrobiyolojik özelliklerindeki değişmeler. Atatürk Üniv Ziraat Fak Derg, 32 (3): 285-292, 2001.

Erdem G, Patır B: Elazığ’da tüketime sunulan tulum peynirlerinde histamin düzeyleri ile bazı kimyasal kalite parametreleri üzerine araştırmalar. F Ü Sağ Bil Vet Derg, 31 (3): 235-241, 2017.