Huseyin Fatih GÜL, Turgut DOLANBAY, Mustafa MAKAV, Emin KARAKURT

Sıçanlarda APAP Kaynaklı Toksisitede EKG, Oksidatif Stres ve Anjiyogenezin Belirlenmesi

Bu çalışma, N-acetyl-p-aminophenol (APAP) toksisitesinin kardiyovasküler sistem üzerindeki etkilerini değerlendirmeyi amaçlamıştır çünkü bu konuda nispeten az sayıda çalışma bulunmaktadır. Çalışma kapsamında her grupta 7 rat (kontrol ve APAP) olacak şekilde 14 adet dişi wistar rat kullanıldı. Kontrol grubuna herhangi bir uygulama yapılmazken APAP grubuna tek doz 1 g/kg N-acetyl-p-aminophenol oral olarak verilmiştir. Apap uygulaması öncesi 0. saat ve uygulama sonrası 1. saat 6. saat 12. saat ve 24. saatte iki gruptaki tüm hayvanlara EKG ölçümü yapıldı. Çalışma sonunda tüm hayvanlar sakrifiye edildi. Biyokimyasal ve histopatolojik analizler için kalp dokusu örnekleri alındı. Doku örneklerinden MDA, GSH, Apelin, Elabela, Meteorin, Endoglin, Keap1 ve Nrf2 ölçümleri yapıldı. Yapılan analizlere göre ekg verilerinde QTc, kalp atım sayısı ve QRS’de APAP grubunda istatistiksel anlamda artış belirlendi. Biyokimyasal verilerde ise APAP grubunda MDA ve Endoglinde anlamlı artış bulunurken GSH, Elabela ve Nrf2’de ise anlamlı bir azalma belirlenmiştir. Histopatolojik olarak APAP grubunda nekroze lezyonlara rastlanmıştır. Sonuç olarak analjezi olarak kullanılan APAP yüksek doz alımlarında kardiyovasküler sistemde kalıcı hasarlara yol açabilmektedir.

Determinate of ECG, Oxidative Stress, and Angiogenesis in APAPInduced Toxicity in Rats

This study was aimed to evaluate the eff ects of N-acetyl-p-aminophenol (APAP) toxicity on the cardiovascular system as there exist relatively a few studies on this matter. The study included 14 female Wistar rats divided into two groups having 7 rats in each (control-APAP). Control group received no medication and APAP group was given single oral dose of 1g/kg APAP. ECG measurements of each animal in either group were obtained before the administration of APAP (0 h) and at the 1st, 6th, 12th and 24th h after the APAP administration. All animals were sacrificed at the end of the study. Heart tissue samples were obtained for biochemical and histopathological analyses. The levels of MDA, GSH, Apelin, Elabela, Meteorin, Endoglin, Keap1, and Nrf2 were measured in the tissue samples. Results revealed a statistically significantly prolonged QTc and QRS intervals and increased heart in the APAP group. A notable increase in MDA and Endoglin, and a significant decrease in GSH, Elabela, and Nrf2 levels occurred in the APAP group. Histopathologically, necrotic lesions were found in the APAP group. The use of high doses of APAP as an analgesic may cause permanent damage in the cardiovascular system.

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