Hasan ORAL, Mushap KURU, Emin KARAKURT, Serpil DAĞ, Ayfer YILDIZ, Hilmi NUHOĞLU, Enver BEYTUT

Köpeklerde Malign Meme Bezi Tümörlerinde Oksidatif Stres Parametrelerinin Varlığı ve Önemi

Bu çalışmada, kanin meme bezi karsinomlarında lipid peroksidasyonuna dayalı oksidatif stres varlığının ve DNA hasar belirteçleri malondialdehit (MDA) ve 8-hidroksi-2’-deoksiguanozin (8-OHdG)’nin immünohistokimya teknikleri kullanılarak değerlendirilmesi amaçlanmıştır. Toplam on malign ve altı normal köpek meme dokusu örneği değerlendirildi. Örnekler %10’luk tamponlu formaldehit solüsyonunda tespit edildi, rutin olarak işlendi, parafin blok içine gömüldü, 5 μm kalınlığında kesitler alındı, Hematoksilen ve Eozin ile boyandı, ışık mikroskobu altında incelendi ve histopatolojik değişiklikleri saptamak için fotoğrafl andı. İmmünhistokimya için Avidin-Biotin-Peroksidaz yöntemi uygulandı. Tüm köpek meme bezi tümörleri, MDA ve 8-OHdG ekspresyonu yönünden immünopozitifti. Kontrol grubuna göre tümör grubunda MDA ve 8-OHdG ekspresyonlarında istatistiksel olarak anlamlı bir artış vardı. Bu çalışma verilerine dayanarak, oksidatif stres bağlamında, lipid peroksidasyonu ve reaktif oksijen türleri (ROT) kaynaklı DNA hasarının köpek meme bezi tümörü gelişimi ile önemli ölçüde ilişkili olduğu önerilmektedir. Ek olarak, köpek meme bezi tümörlerinin tedavisinde antioksidanlar faydalı olabilir.

Presence and Importance of Oxidative Stress Parameters in Malignant Mammary Gland Tumors in Dogs

This study aimed to evaluate the presence of oxidative stress based on lipid peroxidation and the DNA damage markers malondialdehyde (MDA) and 8-hydroxy-2’-deoxyguanosine (8-OHdG) in canine mammary gland carcinomas using immunohistochemistry techniques. A total of ten malignant and six normal canine mammary tissue samples were evaluated. The specimens were fixed in 10% buff ered formaldehyde solution, processed routinely, embedded in paraffin wax, sectioned at 5 μm, stained with hematoxylin and eosin, examined under a light microscope, and photographed to detect histopathological changes. For immunohistochemistry, the avidin-biotin-peroxidase method was performed. All canine mammary gland tumors were immunopositive for MDA and 8-OHdG expression. There was a statistically significant increase in MDA and 8-OHdG expressions in the tumor group compared to the control group. Based on this study data, in the context of oxidative stress, it is proposed that lipid peroxidation and reactive oxygen species (ROS)-induced DNA damage are significantly associated with canine mammary gland tumor development. In addition, antioxidants may be useful in the treatment of canine mammary gland tumors.

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