Mykola KUKHTYN, Vladislav KOZHYN, Victor HORIUK, Zoya MALIMON, Yulia HORIUK, Tetyana YASHCHUK, Sergiy KERNYCHNYI

Activity of Disinfecting Biocides and Enzymes of Proteases and Amylases on Bacteria in Biofilms

The presence of microbial biofilms on the surfaces of medical instruments, operating equipment, prostheses, catheters, technological lines in the food industry is a fact that contributes to the infection of the macroorganism and contamination of raw materials and products. The aim of the work was to investigate the eff ect of disinfecting substances Vantocilu TG and Catamine AB and their combination with enzymes on bacteria in biofilms. In the experiments, we used disinfecting substances Vantocil TG (Arch Biocides LTD, Great Britain) and Catamine AB (Intersintez, Ukraine). Enzymes: Everlase 16 L and Termamyl 300 L (Novozymes, Denmark). It was found that bacteria in biofilms withstood the minimum bactericidal concentration of Vantocil and Catamine, which was set on their planktonic forms. From one mL of wash from the biofilm after exposure to Vantocil were isolated from 1.9×103 to 4.3×103 microbial cells, and after treatment with Catamine from 5.6×103 to 1.7×104 . At the same time, after treatment of biofilms with Vantocil and Catamine together with enzymes, a decrease in the number of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa cells was observed, on average by two orders up to 101 CFU/mL, compared with treatment with biocides only. That is, there is a clear synergy of enzymes and biocides, which ultimately has a more detrimental eff ect on bacteria in biofilms.

Dezenfektan Biyositler ve Proteaz ve Amilaz Enzimlerinin Biyofilmlerdeki Bakteriler Üzerine Aktivitesi

Gıda endüstrisinde tıbbi cihazların, ameliyat ekipmanlarının, protezlerin, kateterlerin, teknolojik alanların yüzeylerinde mikrobiyal biyofilmlerin varlığı, makroorganizma enfeksiyonlarına ve hammadde ve ürünlerin kontaminasyonuna katkıda bulunan bir gerçektir. Bu çalışmanın amacı, dezenfektan maddeler Vantocilu TG ve Catamine AB’nin ve bunların enzimlerle kombinasyonlarının biyofilmlerdeki bakteriler üzerine etkisini araştırmaktır. Deneylerde, Vantocil TG (Arch Biocides LTD, İngiltere) ve Catamine AB (Intersintez, Ukrayna) dezenfektan maddeleri ile Everlase 16 L ve Termamyl 300 L (Novozymes, Danimarka) enzimlerini kullandık. Biyofilmlerdeki bakterilerin, planktonik formlarına karşı uygulanan Vantocil ve Catamine’nin minimum bakterisidal konsantrasyonlarına dayanıklılık gösterdiği saptandı. Vantocil ile sağaltımdan sonra bir mL biyofilm yıkantısından 1.9x103 ile 4.3x103 arası mikroorganizma ve Catamin ile sağaltımdan sonra 5.6x103 ile 1.7x104 arası mikroorganizma izole edildi. Aynı zamanda, biyofilmlerin enzimlerle birlikte Vantocil ve Catamine ile sağaltımından sonra, Staphylococcus aureus, Escherichia coli ve Pseudomonas aeruginosa bakteri sayılarında sadece biyositlerin kullanıldığı sağaltım ile kıyaslandığında ortalama olarak 101 CFU/mL’ye kadar iki kat bir azalma gözlendi. Sonuçta, biyofilmlerdeki bakteriler üzerine daha hasar verici bir etkiye sahip açık bir enzim ve biyosit sinerjisi mevcuttu.

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