Gamze Sevri EKREN AŞICI, Funda KIRAL, İrem BAYAR, AYŞEGÜL BİLDİK, Pınar Alkım ULUTAŞ

Cytotoxic and Apoptotic Eff ects of Curcumin on D-17 Canine Osteosarcoma Cell Line

Cancer is a major health problem in dogs. Types of cancer seen in dogs include melanoma, Non-Hodgkin lymphoma, osteosarcoma, soft tissuesarcomas and prostate, breast, lung and colorectal carcinomas. Osteosarcoma (OSA) is the most common malignant primary bone tumor indomestic dogs. It constitutes 85% of skeletal tumors. It is derived from primitive bone cells that occur in both the appendicular (~75%) andaxial (~25%) skeleton. The present study was intended to determine the cytotoxic and apoptotic eff ects of curcumin administration at certaindoses and in certain periods on D-17 canine osteosarcoma cells. Canine osteosarcoma cells were treated with curcumin and the eff ects ofit on proliferation were determined by WST-1, apoptosis by caspase 3/7 activity (MuseCaspase 3/7) and the ratio of proapoptotic Bax geneto antiapoptotic Bcl-2 gene expression level by qRT-PCR. Our data demonstrated that curcumin decreased cell proliferation and viability,ultimately inducing caspase 3/7 mediated apoptosis in treated D-17 canine osteosarcoma cells. Furthermore, the application of curcuminon canine osteosarcoma cells downregulated the expression of Bcl-2 and upregulated the expression of proapoptotic gene Bax. Thus, theseresults may provide a basis for further study of curcumin in the treatment of canine osteosarcoma.

Kurkuminin D-17 Köpek Osteosarkom Hücre Hattı Üzerindeki Sitotoksik ve Apoptotik Etkileri

Kanser, köpeklerde büyük bir sağlık sorunudur. Köpeklerde görülen kanser türleri arasında melanom, Non-Hodgkin lenfoma, osteosarkom, yumuşak doku sarkomları ve prostat, meme, akciğer ve kolorektal karsinomlar bulunur. Osteosarkom (OSA) evcil köpeklerde en sık görülen kötü huylu birincil kemik tümörüdür. İskelet tümörlerinin %85’ini oluşturur. Hem apendiküler (~%75) hem de aksiyal (~%25) iskelette meydana gelen ilkel kemik hücrelerinden türemektedir. Bu çalışmanın amacı, belirli dozlarda ve belirli periyotlarda kurkumin uygulamasının ardından D-17 köpek osteosarkom hücreleri üzerindeki sitotoksik ve apoptotik etkilerini saptamaktır. Kurkumin uygulanan köpek osteosarkom hücrelerinde proliferasyon üzerindeki etkileri WST-1 analiziyle, apoptotik etkileri ise kaspaz 3/7 aktivitesi (MuseCaspase 3/7) ve proapoptotik Bax geninin antiapoptotik Bcl-2 gen ekspresyon düzeyine oranı ile belirlendi. Verilerimiz kurkuminin D-17 köpek osteosarkom hücrelerinde hücre proliferasyonunu ve canlılığını azalttığını, kazpaz 3/7 aracılığıyla apoptozu indüklediğini göstermektedir. Bunun yanında kurkuminin köpek osteosarkom hücrelerine uygulanması Bcl-2’nin ekspresyonunu azalttı ve proapoptotik gen Bax ekspresyonunu arttırdı. Bu nedenle, bu sonuçlar, köpek osteosarkoma tedavisinde kurkumin ile ilgili daha fazla çalışma için bir temel sağlayabilir.

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