FUNDA YİĞİT, Elif İ. İKİTİMUR-ARMUTAK, Osman B. Burak ESENER, EBRU GÜREL GÜREVİN, Banu İŞBILEN BAŞOK, M.Başak ULKAY, ABİT AKTAŞ

Sıçan Testisinde 3-Metil-4-Nitrofenol İle Oluşturulan Hasara Karşı

Bu çalışmada beş günlük deneysel bir periyotla Morinda citrifolia (Noni)'nın sıçan testislerinde 3-metil 4-nitrofenol (PNMC) toksisitesine karşı koruyucu etkisi araştırıldı. Ellialtı adet yetişkin erkek Sprague-Dawley sıçanı eşit sayıda yedi deneysel, bir kontrol grubuna ayrıldı (n:7). Bir gruba sadece Noni uygulandı. Altı deneysel grupta testiste doku hasarı farklı dozlarda (1, 10 and 100 mg/kg) subkutan PNMC enjeksiyonu ile indüklendi ve üç gruba Noni tedavisi uygulandı (her sıçan için 2ml gavaj ile). Altıncı günde tüm sıçanlar sakrifiye edilerek kan ve testis doku örnekleri toplandı. Serum testosteron, FSH ve LH seviyeleri değerlendirildi. Testis dokuları, tubuler çap, seminifer epitel yoğunluğu, lumen aralığı ve interstisyel doku açısından histomorfometrik olarak incelendi ve oksitatif hasarı değerlendirmek üzere indüklenebilir nitrik oksit sentaz (iNOS) ve endotelyal nitrik oksit sentaz (eNOS) belirteçleri ile immunohistokimyasal olarak işaretlendi. Histomorfometrik olarak en şiddetli doku hasarı 10 mg/kg'lık PNMC grubunda gözlendi. Doku hasarı bu gruba karşılık gelen tedavi (Noni ile) grubunda anlamlı ölçüde iyileşti. iNOS ve eNOS düzeyleri tüm PNMC gruplarında yükseldi ve Noni tedavisi ile aynı değerler düşüş gösterdi. Noni'nin oksidatif hasar bakımından en çok 100mg/kg'lık PNMC grubunda etkin olduğu izlendi. Serum hormon değerleri anlamlı sonuçlar vermedi. Sonuç olarak, Noni, sıçan testislerinde PNMC ile indüklenen doku hasarını azalttı.

Protective Effect of Morinda citrifolia (Noni) on 3-methyl-4-nitrophenol-induced Injury in Rat Testes [1]

In this study, the protective effect of Morinda citrifolia (Noni) against 3-methyl-4-nitrophenol (PNMC) toxicity in rat testes was investigated with an experimental period of five days. Fifty-six adult male Sprague-Dawley rats were allocated into seven experimental groups and a control (n:7). One group received only Noni. Testicular tissue injury of six experimental groups was induced by subcutaneous injection of PNMC at three different doses (1, 10 and 100 mg/kg) and three received Noni treatment (2 ml per rat by gavage). On day six all rats were sacrificed and then blood samples and testis tissues were collected. Serum testosterone, FSH and LH levels were assessed. Testicular tissues were evaluated histomorphometrically in terms of tubular diameter, seminiferous epithelium density, luminal space and interstitial tissue and immunohistochemically labelled with inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) markers to assess oxidative damage. Histomorphometrically, most severe tissue injury was observed in the group of 10 mg/kg PNMC. Tissue injury improved significantly in its corresponding treatment group (with Noni). iNOS and eNOS levels increased in all PNMC groups and decreased with Noni treatments. Noni was most effective in the group of 100mg/kg PNMC in terms of oxidative damage. Serum hormone levels revealed no significant results. In conclusion, Noni reduced PNMC-induced tissue injury in rat testes.

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