Rizwana Abdul GHANI, Naureen Aslam KHATTAK, Muhammad SAQLAIN, Muhammad Javaid ASAD, Azra KHANUM, Syed Muhammad Saqlan NAQVI, Ghazala Kaukab RAJA

Comparison of the Common Immunogenic Protein Components of Pasteurella multocida Serotypes B:2 and B: 3,4

Pasteurella multocida B serotipi Güney Asya'daki sığır ve mandalarda yaygın olarak görülen ve kanda septisemi ile seyreden hemorajik septisemi (HS)'nin en önemli nedenidir. Hastalık için günümüzde kullanılan aşılar yeteri kadar etkili değildir ve sadece sınırlı bir sürede etkisini göstermektedir. Canlı/subunit aşılarda kullanılmak üzere Pasteurella multocida'nın immunojenik komponentlerinin belirlenmesi için yapılan çalışmamızda, B:2 ve B:3,4 serotiplerinin dış membran proteinleri (OMP) izole edilmiş ve bunların profilleri karşılaştırılmıştır. İmmunbloting sonucunda 39.0, 33.5 ve 31.0 kDa moleküler ağırlıklarında 3 adet ortak immunojenik polipeptit belirlenmiştir. İdentifiye edilen dış membran proteinlerinin immunojenitesi ayrıca bunlardan elde edilen antiserumlar ile doğrulanmıştır. Bu çalışmada identifiye edilen antijenik OMP'ler uzun süre etki gösteren yeni, güvenli ve etkin aşıların formülasyonlarının geliştirilmesine katkı sağlayacaktır.

Pasteurella multocida B:2 ve B:3,4 Serotiplerinin Ortak İmmunojenik Protein Komponentlerinin Karşılaştırılması

Serotype B of Pasteurella multocida is a major agent of haemorrhagic septicaemia (HS), a form of blood poisoning widespread in cattle and buffalo from Southern Asia. The vaccines currently used against the disease are not potent enough, being effective only for a limited duration only. In an attempt to identify immunogenic components of P. multocida for use as live/subunit vaccines, the outer membrane proteins (OMPs) of B:2 and B:3,4 serotypes were isolated and their profiles compared. Three common immunogenic components; polypeptides of molecular weight (MW) 39.0, 33.5, 31.0 kDa, were identified by immunoblotting. The immunogenicity of identified OMPs was also confirmed using antisera generated against them. The antigenic OMPs identified in current research could be explored in the formulation of new, safe and effective vaccines for indigenous buffalo and cattle breeds with a prolonged efficacy.

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