ELISA PROCEDURES, ENES ATMACA, FEHMİ YAZICI, DİLEK GÜVENÇ, OSMAN GÜL, Muhammet DERVİŞOĞLU

Comparative Analysis of Aflatoxin M1 in Marketed Butter by ELISA and HPLC

Bu çalışmada Karadeniz bölgesinde tüketime sunulan tereyağlarında iki farklı analitik yöntem karşılaştırarak aflatoksin M1 varlığının araştırılması amaçlandı. Bu amaçla, toplam 40 tereyağı numunesi ELISA testi ile tarandı ve ardından doğrulayıcı bir yöntem olannumunelerin temizleme aşamasında immunoafinite kolonların kullanıldığı floresan detektörlü yüksek performanslı sıvı kromatografi (IAC/HPLC-FLD) ile analizleri yapıldı. Yapılan çalışma ile herhangi bir tereyağı numunesinde AFM1 tespit edilmemiş olup, Karadeniz bölgesinde üretilen tereyağlarının AFM1 açısından iyi kalitede olduğu sonucuna varıldı. Ayrıca, elde edilen sonuçlara göre tereyağında AFM1 tespitinde kullanılan IAC/HPLC-FLD metodunun doğrulayıcı ve hassas bir yöntem olduğu ortaya konulmuştur.

Tereyağlarında Aflatoksin M1 düzeylerinin ELISA ve HPLC ile Karşılaştırmalı Analizi

The aim of this study was to examine the presence of aflatoxin M1 (AFM1) in butter samples marketed in the Black Sea Region of Turkey with two complementary analytical methods to improve accuracy. A total of 40 samples were analysed by enzyme-linked immuno absorbent assay (ELISA) as the screening test and high performance liquid chromatography (HPLC) with fluorescence detection (FLD) after immunoaffinity column (IAC) clean-up as the confirmatory method. Results indicated that butter samples in the Black Sea region were of good quality with respect to AFM1, with no contamination detected. Furthermore, the accurate and sensitive IAC/HPLC-FLD method was confirmed as being appropriate for the detection of AFM1 in butter.

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Sugiyama K, Hiraoka H, Sugita-Konishi Y: Aflatoxin M1 contamination in raw bulk milk and the presence of aflatoxin B1 in corn supplied to dairy cattle in Japan. J Food Hyg Soc Jpn,49, 352-355, 2008. DOI: 10.3358/ shokueishi.49.352
Anfossi L, Baggiani C, Giovannoli C, Giraudi G: Occurrence of aflatoxin M1 in dairy products. In, Torres-Pacheco I (Ed): Aflatoxins - Detection, Measurement and Control. 1-20, Intech, Rijeka, 2011. DOI: 10.5772/22724
Fallah AA, Rahnama M, Jafari T, Saei-dehkordi SS: Seasonal variation of aflatoxin M1 contamination in industrial and traditional Iranian dairy products. Food Control, 22, 1653-1656, 2011. DOI: 10.1016/j. foodcont.2011.03.024
Var I, Kabak B: Detection of aflatoxin M1 in milk and dairy products consumed in Adana, Turkey. Int J Dairy Technol, 62, 15-18, 2009. DOI: 1111/j.1471-0307.2008.00440.x
Wacoo AP, Wendiro d, Vuzi PC, Hawumba JF: Methods for detection of aflatoxins in agricultural food crops. J Appl Chem, 1-15, 2014. DOI: 1155/2014/706291
Bhat R, Rai RV, Karim AA: Mycotoxins in food and feed: Present status and future concerns. Compr Rev Food Sci, 9, 57-81, 2010. DOI: 1111/j.1541-4337.2009.00094.x
Yao H, Hruska Z, diana di Mavungu J: Developments in detection and determination of aflatoxins. World Mycotoxin J, 8, 181-191, 2015. DOI: 3920/WMJ2014.1797
Awad WA, Ghareeb K, Böhm J: Occurrence, health risks and methods of analysis for aflatoxins and ochratoxin A. J Vet Anim Sci, 2, 1-10, 2012.
Sakuma H, Kamata Y, Sugita-Konishi Y, Kawakami H: Method for determination of aflatoxin M1 in cheese and butter by HPLC using an immunoaffnity column. Food Hyg Safe Sci, 52, 220-225, 2011.DOI:10.3358/ shokueishi.52.220
Aydemir Atasever M, Atasever M, Özturan K, Urçar S: Determination of aflatoxin M1 level in butter samples consumed in Erzurum, Turkey. Kafkas Univ Vet Fak Derg, 16 (Suppl-A): S159-S162, 2010. DOI: 10.9775/ kvfd.2010.2671
Tekinşen KK, Uçar G: Aflatoxin M1 levels in butter and cream cheese consumed in Turkey. Food Control, 19, 27-30, 2008. DOI:10.1016/j. foodcont.2007.01.003
Aycicek, H, Aksoy A, Saygi S: Determination of aflatoxin levels in some dairy and food products which consumed in Ankara, Turkey. Food Control, 16, 263-266, 2005. DOI:10.1016/j.foodcont.2004.03.004
Tosun H, Ayyildiz T: Occurence of aflatoxin M1 in organic dairy products. Qual Assur Safe of Crop, 5, 215-219, 2013. DOI:10.3920/ QAS2012.0147
Bakirci I: A study on the occurrence of aflatoxin M1 in milk and milk products produced in Van province of Turkey. Food Control, 12, 47-51, DOI:10.1016/S0956-7135(00)00020-7
Krska R, Schubert-Ullrich P, Molinelli A, Sulyok M, Macdonald S, Crews C: Mycotoxin analysis: An update. Food Addit Contam, 25, 152- , 2008. DOI: 10.1080/02652030701765723
Krska R, Welzig E, Berthiller F, Molinelli A, Mizaikoff B: Advances in the analysis of mycotoxins and its quality assurance. Food Addit Contam, , 345-353, 2005. DOI: 10.1080/02652030500070192
Herzallah SM: Determination of aflatoxins in eggs, milk, meat and meat products using HPLC fluorescent and UV detectors. Food Chem, 114, 1146, 2009. DOI: 10.1016/j.foodchem.2008.10.077
Hussain I, Anwar J, Asi MR, Munawar MA, Kashif M: Aflatoxin M1 contamination in milk from five dairy species in Pakistan. Food Control, , 122-124, 2010. DOI: 10.1016/j.foodcont.2008.12.004