MUHİTDİN YILMAZ, EVREN KOÇ, ONUR ATAKİŞİ, AHMET HARMANKAYA, YUSUF ERSAN, Musa KARAMAN, MEHMET ÇİTİL

The Protective Effects of L-Carnitine Against Lead (II) Acetate Toxicity in Capoeta capoeta (Guldensteadt 1773)

Çalışmada, Capoeta capoeta'da Kurşun (II) asetat (Pb(C2H3O2)2) toksisitesine karşı L-karnitinin (LK) koruyucu etkileri histopatolojik, elektroforetik ve biyokimyasal yöntemlerle incelendi. Kars Çayı'ndan yakalanan balıklar 500 L'lik tanklarda her grupta 10'ar adet balık bulunan 4 gruba ayrıldı. Kurşun ve sıvı LK suya ilave edildi. I. gruptaki balıklar kontrol grubu olarak belirlendi. II. gruptaki balıklara 10 gün süreyle 1 mg/L kurşun asetat uygulandı. III. gruptaki balıklara 100 mg/L LK uygulandı. IV. gruptaki balıklar ise 1 mg/L kurşun ve 100 mg/L dozunda LK uygulandı. Karaciğer, solungaç, bağırsak ve böbrek dokularında kurşun asetatın toksik etki gösterdiği ve buna karşı L-karnitin uygulamasının koruyucu etki göstererek oluşan bu dejenerasyonların şiddetini azalttığı gözlemlendi. Elektroforetik incelemede, kurşun asetatın birçok protein bandında inhibisyona neden olduğu, LK uygulaması sonucunda da protein ekspresyonlarında artış meydana geldiği saptandı. Kurşun uygulanan grupta total protein düzeyinin yüksek, kurşun + LK verilen grubun ise düşük olduğu belirlendi (P

Capoeta capoeta (Guldensteadt 1773)'larda Kurşun (II) Asetat Toksisitesine Karşı L-Karnitinin Koruyucu Etkileri

In this study, the protective effects of L-carnitine (LC) against Lead (II) acetate (Pb(C2H3O2)2) toxicity in Capoeta capoeta were investigated by means of histopathologically, via electrophoretic and biochemical methods. Fish caught from Kars creek were divided into four groups, which include 10 fish each in 500 L tanks. Lead and liquid LC were added to water. Fish in the first group were adjusted as control. Fish in the II. group were applied 1 mg/L lead (as Lead (II) acetate) for 10 days. Fish in the III. group were administered 100 mg/L LC. Fish in the IV. group were administered 1 mg/L lead and 100 mg/L LC. Degenerations in liver, gill, intestine and kidney tissues were observed to reduce LC administration against the toxic effects of lead acetate. Electrophoretically, inhibitions of some protein bands in the group, which was applied lead acetate, were caused, and increases in protein expression in the group, which was applied L-carnitine, have occurred. While high a level of total protein in the group that was administered lead was found; in the group that was treated lead + LC, it was found to be lower (P<0.05). Levels of globulin in the group that was administered LC + lead were observed to be significantly lower (P<0.05). Total oxidant capacity (TOC) in lead treatment group were higher than the control group, TOC levels in lead + LC treatment group were determined to be between the control and lead group. LC was concluded to show a protective effect on Capoeta capoeta that were exposed to lead.

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World Health Organization: Environmental Health Criteria 165. International Programme on Chemical Safety, Geneva, 1995.
Balci A, Türkoğlu M: Heavy metals in sediments from Izmir Bay, Turkey. Mar Pollut Bull, 26, 106-107, 1993. DOI: 10.1016/0025-326X(93)90100-X
Kutlu M, İşcan A, Tanatmış M: Structural changes of Artemia parthenogenetica (Bowen and Sterling, 1978) (Branchiopoda-Anostraca) exposed to lead acetate. Food Chem Toxicol, 46, 3770-3773, 2008. DOI: 1016/j.fct.2008.09.060
Fracasso ME, Perbellini L, Soldà S, Talamini G, Franceschetti P: Lead induced DNA strand breaks in lymphocytes of exposed workers: Role of reactive oxygen species and protein kinase C. Mutat Res Genet Toxicol Environ Mutagen, 515, 159-169, 2002. DOI: 10.1016/S1383- (02)00012-8
Deng K, Wong CW, Nolan JV: Long-term effects of early-life dietary L-carnitine on lymphoid organs and immune responses in Leghorn-type chickens. J Anim Physiol Anim Nutr, 90, 81-86, 2006. DOI: 10.1111/j.1439- 2005.00569.x
Harpaz S: L-carnitine and its attributed functions in fish culture and nutrition-A review. Aquaculture, 249, 3-21, 2005. DOI: 10.1016/j. aquaculture.2005.04.007
Rebouche CJ: Carnitine function and requirements during the life cycle. FASEB J, 6, 3379-3386, 1992.
Fritz IB, Arrigoni-Martelli E: Sites of action of carnitine and its derivatives on the cardiovascular system: Interactions with membranes. Trends Pharmacol Sci, 14, 355-360, 1993. DOI: 10.1016/0165-6147(93) Y
Yavuz H, Kurtoğlu F: The effects of L-carnitine on blood and tissue parameters of male rats fed with different levels of fish oil. Eurasian J Vet Sci, 30, 138-144, 2014. DOI: 10.15312/EurasianJVetSci.201436513
Crentsil V: Mechanistic contribution of carnitine deficiency to geriatric frailty. Ageing Res Rev, 9, 265-268, 2010. DOI: 10.1016/j.arr.2010.02.005
Robert R, Michael JD: Enzyme Assays. pp. 225-332, Oxford University Press, Newyork, 1992.
Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685, 1970. DOI: 1038/227680a0
O'Farrell PH: High resolution two-dimensonal electrophoresis of biological properties and significance. Comp Biochem Physiol, 88, 497- , 1975.
Weber K, Pringle JR, Osborn M: Measurement of molecular weights by electrophoresis on SDS-acrylamide gel. Methods Enzymol, 26, 3-27, DOI: 10.1016/S0076-6879(72)26003-7
Erel O: A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin Biochem, 37, 277-285, 2004. DOI: 10.1016/j. clinbiochem.2003.11.015
Sieg DJ, Billings RE: Lead/cytokine-mediated oxidative DNA damage in cultured mouse hepatocytes. Toxicol Appl Pharmacol, 142, 106-115, DOI: 10.1006/taap.1996.8033
Daggett DA, Oberley TD, Nelson SA, Wright LS, Kornguth SE, Siegel FL: Effects of lead on rat kidney and liver: GST expression and oxidative stress. Toxicology, 128, 191-206, 1998. DOI: 10.1016/S0300- X(98)00080-8
Shalan MG, Mostafa MS, Hassouna MM, El-Nabi SH, El-Refaie A: Amelioration of lead toxicity on rat liver with vitamin C and silymarin supplements. Toxicology, 206, 1-15, 2005. DOI: 10.1016/j.tox.2004.07.006
El-Sokkary GH, Abdel-Rahman GH, Kamel ES: Melatonin protects against lead-induced hepatic and renal toxicity in male rats. Toxicology, , 25-33, 2005. DOI: 10.1016/j.tox.2005.05.003
Mutlu N, Ersan Y, Nur G, Koç E: Protective effect of caffeic acid phenethyl ester against lead acetate-induced hepatotoxicity in mice. Kafkas Univ Vet Fak Derg, 17 (Suppl. A): S1-S5, 2011. DOI: 10.9775/ kvfd.2010.2717
Yılmaz M, Ersan Y, Karaman M, Özen H, Koç E, Necefoğlu H: Toxic effects of cobalt parahydroxybenzoate on tissue histopathology and serum proteins in Capoeta capoeta capoeta. Fresen Environ Bull, 17, 1322- , 2008.
Koç E, Yılmaz M, Ersan Y, Alaş A: Detection of the of effects of hexavalent chromium by histopathological and electrophoretic methods on Capoeta capoeta (Guldenstaedt 1773) and Squalius cephalus (Linnaeus ). Kafkas Univ Vet Fak Derg, 19, 979-984, 2013. DOI: 10.9775/ kvfd.2013.9210
Davies KJ: Protein damage and degradation by oxygen radicals. I. general aspects. J Biol Chem, 262, 9895-9901, 1987.
Aktaş Ö, Eskiocak S, Sayılan Özgün G, Yalçın Ö, Süt N: The effects of L-carnitine on acetaminophen induced hepatotoxicity in rats. Turk J Biochem, 38, 475-482, 2013. DOI: 10.5505/tjb.2013.92063
Chen G, Zhang M, Zhang J, Dong H, Zhou H, Tang B, Huang J, Shi G, Jiang L, Wu Z: The effects of different levels of dietary protein and L-carnitine on blood sugar and lipids of the new gift strain of juvenile nile tilapia (Oreochromis niloticus). Scientific World J. 9, 1197-1205. 2009. DOI: 1100/tsw.2009.129
Hussain AM, Mitra AK: Effect of reactive oxygen species on the metabolism of tryptophan in rat brain: Influence of age. Mol Cell Biochem, , 145-153, 2004. DOI: 10.1023/B:MCBI.0000012849.16750.00
Yapar K, Kart A, Karapehlivan M, Atakisi O, Tunca R, Erginsoy S, Citil M: Hepatoprotective effect of L-carnitine against acute acetaminophen toxicity in mice. Exp Toxicol Pathol, 59, 121-128, 2007. DOI: 10.1016/j. etp.2007.02.009
Augustyniak A, Skrzydlewska E: L-Carnitine in the lipid and protein protection against ethanol-induced oxidative stress. Alcohol, 43, 217-223, DOI: 10.1016/j.alcohol.2008.12.005
Guzmán-Guillén R, Prieto AI, Vázquez CM, Vasconcelos V, Cameán AM: The protective role of L-carnitine against cylindrospermopsin- induced oxidative stress in tilapia (Oreochromis niloticus). Aquat Toxicol, , 141-150, 2013. DOI: 10.1016/j.aquatox.2013.02.011
Şıktar E, Ekinci D, Şıktar E, Beydemir Ş, Gülçin İ, Günay M: Protective role of L-carnitine supplementation against exhaustive exercise induced oxidative stress in rats. Eur J Pharmacol, 668, 407-413, 2011. DOI: 1016/j.ejphar.2011.07.032