OĞUZ MERHAN, AYLA ÖZCAN, Emine ATAKİŞİ, Metin ÖĞÜN, ABDULSAMED KÜKÜRT

The Effect of ?-carotene on Acute Phase Response in Diethylnitrosamine Given Rabbits

Bu çalışmada, tavşanlara yüksek dozda verilen dietilnitrozaminin (DEN) toksik etkisine karşı oluşabilecek akut faz yanıt (AFY) üzerine ?-karotenin etkisinin araştırılması amaçlandı. Materyal olarak kullanılan 21 adet, 5-7 aylık Yeni Zelanda ırkı tavşan, her birinde 7 adet olacak şekilde 3 gruba ayrıldı. Kontrol: Tek doz %0.9'luk NaCl solüsyonu intraperitoneal (İP), Grup I: Tek doz 100 mg/kg DEN (İP), Grup II: Tek doz 100 mg/kg DEN (İP) + 2 mg/kg/gün ?-karoten 7 gün boyunca günlük oral yolla verildi. Tavşanlardan enjeksiyondan sonraki 1., 4. ve 7. günlerde kan örnekleri alındı. Seruloplazmin düzeyi Colombo ve Richterich yöntemiyle, serum amiloid A (SAA) ve haptoglobin ELISA kit ile albümin, demir (Fe), doymamış demir bağlama kapasitesi (DDBK), AST, ALT ticari kit kullanılarak kolorimetrik yöntem ile, total demir bağlama kapasitesi (TDBK) ve transferin doyumu (TD) ise Fe ve DDBK üzerinden formülle hesaplanarak saptandı. Haptoglobin ve SAA düzeylerinin 1., 4. ve 7. günlerde (P

Dietilnitrozamin Verilen Tavşanlarda ?-karotenin Akut Faz Yanıta Etkisi

The aim of this study was to investigate the effect of ?-carotene in acute phase response (APR) in rabbits which were administered high doses of toxic diethylnitrosamine (DEN). Twenty-one New Zealand race rabbits at 5-7 months of age were divided into 3 groups each having 7 ones. Control group received single dose of 0.9% NaCl solution intraperitoneally (IP); Group 1 received single dose of 100 mg/kg DEN (IP); Group 2 received both 100 mg/kg DEN (IP) and 2 mg/kg/day ?-carotene orally for 7 days. After applying administrations, blood samples were obtained on 1st, 4th, and 7th days. The levels of ceruloplasmin was determined by the Colombo and Richterich technique; serum amyloid A (SAA) and haptoglobin using ELISA; iron (Fe) and unsaturated iron binding capacity (UIBC), AST and ALT using a commercial colorimetric kit; total iron binding capacity (TIBC) and transferrin saturation (TS) were determined using a formula based on Fe and UIBC. We determined that the levels of haptoglobin and SAA on 1st, 4th, and 7th days (P<0.01) and the level of ceruloplasmin on 1st and 4th days significantly increased (P<0.01) relative to the controls. On the other hand, the levels of TS decreased on 4th and 7th days relative to control (P<0.05). These results suggest that ?-carotene has a protective role on APR resulted from the toxic effect of DEN.

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