Krzysztof GÓRSKI, Stanis?aw KONDRACKI, Anna WYSOKI?SKA, Anna NAZARUK

The Importance of Ejaculate Volume for the Physical Parameters of Ejaculates and Sperm Morphology of Hypor Boars

Çalışmada Hypor erkek domuzların sperm morfolojisi ve ejakülatın fiziksel özellikleri üzerine ejakülat hacminin etkilerini incelemek amaçlandı. Bu amaçla, 12 adet Hypor ırkı suni tohumlama domuzundan alınan 114 ejakülat incelendi. Ejakülatlar hacme göre gruplandırıldı: Numuneler, 251 ml ve altında (Grup 1), 251-310 ml (Grup 2) ve 310 ml ve üstünde (Grup 3) olarak ayrıldı. Sperma, major ve minor morfolojik sperm anomalilerinin sıklığı ve temel fiziksel özellikleri yönünden değerlendirildi. Ayrıca, spermatozoa'nın morfolojik yapısına ait değerler saptandı. Ejakülat hacmi artış ve ejakülasyondaki sperm konsantrasyonunda az miktarda düşüş ile, motilite ve toplam sperm sayısında bir artışla beraber bulundu. En yüksek hacimli ejakülatlarda düzgün yapıda morfolojiye sahip daha fazla sayıda spermatozoa saptandı. Ayrıca, daha yüksek ejakülat hacmiyle birlikte spermatozoonların özellikle baş kısmında olmak üzere ebatlarının büyüdüğü saptandı. Spermatozoonun baş uzunluğu ve genişliği ile çevre uzunluğu ve yüzey alanı büyüdü. Ejakülat hacmi Hypor domuz spermatozoonlarının şekli üzerinde bir etkiye sahipti. Ejakülat hacminin artmasıyla spermatozoa başı daha oval bir şekil aldı. Ek olarak, hacmin artmasıyla ejakülattaki spermatozoa kuyruk uzunluğu ile daha uzun başa sahip olma arasında bir ilişki saptandı.

Hypor Domuzlarında Sperm Morfolojisi ve Ejakülatların Fiziksel Özellikleri Yönünden Ejakülat Hacminin Önemi

The study aimed at analysing the effects of ejaculate volume on the physical parameters of ejaculates and the sperm morphology of Hypor boars. The analyses involved 114 ejaculates collected from 12 Hypor insemination boars. The ejaculates were classified according to the criterion of ejaculate volume. Three groups were specified: ejaculates with a volume of 251 ml or lower (Group I), ejaculates with a volume of 252-310 ml (Group II), and ejaculates with a volume of 311 ml or higher (Group III). The ejaculates were assessed to identify the basic physical traits and determine the incidence of morphological abnormalities in the spermatozoa, specifying major and minor abnormalities. Furthermore, the morphological structure indices for the spermatozoa were also calculated. Rising ejaculate volume accompanied with a rise in the total number and motility of spermatozoa, and a simultaneous slight fall in sperm concentration in the ejaculates. The ejaculates with the highest volumes turned out to contain more morphologically well-formed spermatozoa. We also determined that rising ejaculate volume is accompanied with increasing sperm dimensions, especially those of the head. The increased parameters were the length and the width of sperm heads, as well as their perimeters and areas. Ejaculate volume has an impact on the shape of Hypor boar spermatozoa. As the ejaculate volume increases, the shape of sperm heads becomes increasingly more oval. Additionally, spermatozoa in ejaculates with greater volumes have larger heads in relation to flagellum length.

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