ÖMER KIZIL, MELTEM KIZIL, ABDURRAUF YÜCE

Sancı semptomlu atlarda plazma oksidatif stres parametreleri

Bu çalışma, sancı semptomlu atlarda plazma oksidatif stres parametrelerinin belirlenmesi amacıyla yapılmıştır. Bu amaçla akut sancı semptomlu 15 ve kontrol amacıyla da 10 adet klinik olarak sağlıklı at kullanılmıştır. Plazmada glutatiyon peroksidaz (GPx), indirgenmiş glutatiyon (GSH), katalaz (CAT) ve lipid peroksidasyon (MDA) düzeyleri spektrofotometrik olarak tayin edilmiştir. Gruplar arasında (sancılı ve sağlıklı atlar) MDA, Glutatiyon peroksidaz ve indirgenmiş glutatiyon bakımından önemli istatistiksel farklılık saptanmıştır. Katalaz bakımından ise, sağlıklı atlarda daha yüksek düzeyler saptanmasına rağmen gruplar arasında istatistiksel farklılık saptanamamıştır. Bu çalışma, sancı semptomlu atlarla kontroller arasında plazma oksidatif stres parametreleri açısından önemli farklılıkların olduğuna dikkat çekmektedir.

Anahtar Kelimeler:

lipit peroksidasyonu, katalaz, karın ağrısı, glutatyon, glutatyon peroksidaz, at, malonaldehit, stres, stres yanıtı

Plasma oxidative stress parameters in horses with colic symptoms

This study was aimed at determining the plasma oxidative stress parameters in horse with colic symptom. The study involved fifteen horses with acute colic and ten healthy horses as control. Plasma glutathione peroxidase, reduced glutathione, catalase and lipid peroxidation levels were spectrophotometrically determined. Malondiadehyde (MDA), glutathione peroxidase (GsHPx) and reduced glutathione (GSH) concentrations significantly different between the groups. Although catalase (CAT) levels was higher in healthy horses, this difference was not statistically significant. This study has highlighted important differences in plasma oxidative stres parameters between horse with colic and healthy controls.

Keywords:

lipid peroxidation, catalase, colic, glutathione, glutathione peroxidase, horses, malonaldehyde, stress, stress response,

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