NADİDE NABİL KAMİLOĞLU, Ebru BEYTUT, AYSEL GÜVEN

Lipid peroxidation and antioxidant defence of mammary glands and supramammary lymph nodes of cattle infected with brucella abortus

Bu çalışmada, Brucella abortus (B. abortus) ile enfekte olmuş sığırların meme ve meme altı lenf bezlerindeki redükte glutatyon (GSH), lipid peroksidasyon indikatörü tiyobarbiturik asit reaktif substrat (TBARS) düzeyleri, glutatyon peroksidaz aktivitesi (GSH-Px, EC. 1.11.1.9), ile P-karoten, A ve E vitaminlerinin düzeylerinin belirlenmesi amaçlanmıştır. Bunun için, Kars ve yöresinin değişik köylerinden ve bölgedeki iki kesimhaneden sağlanan 20 sağlıklı, 20 ikinci trimesterde düşük yapmış ,ve serolojik testlerle B. abortus ile enfekte olduğu belirlenen toplam 40 sığırdan alınan numuneler kullanılmıştır. B. abortus ile enfekte sığırların meme ve meme altı lenf bezlerinde TBARS düzeyleri istatistiksel olarak yüksek olarak (p

Anahtar Kelimeler:

lenf düğümleri, retinol, E vitamini, Brucella abortus, antioksidanlar, beta-karoten, brusellozis, sığır, savunma mekanizması, enzim aktivitesi, glutatyon peroksidaz, bağışık yanıt, lipit peroksidasyonu, meme bezleri

Brucella abortus ile enfekte sığırların meme ve meme altı lenf bezlerinde lipid peroksidasyon ve antioksidan savunma

The aim of this study was to determine the levels of (3-carotene, vitamin A, E, the activity of glutathione peroxidase (GSH-Px, EC. 1.11.1.9) and the levels of reduced glutathione (GSH) and thiobarbituric acid reactive substances (TBARS) as indicator of lipid peroxidation in the supramammary lymph nodes and mammary glands of cattle infected with Brucella abortus (B. abortus). In this study, samples of 20 healthy and 20 heifers aborted in second trimaster of gestation found to be brucella-positive by serological tests were used. The cattle were brought from various villages in Kars region and slaughtered in two local abattoirs. Our results show that the TBARS levels in the cattle infected with B. abortus were significantly (p<0.001) increased and GSH-Px activity and GSH levels were significantly (0.01) decreased as compared to the healthy cattle. Also, vitamin E, A (P<0.01) and (3-carotene (P<0.001) levels of the infected animals were significantly decreased as compared to the healthy cattle. These results indicate that during immun response against B. abortus- induced bovine brucellosis caused oxidative stress with the elevation in LPO was reduced the levels of the vitamin E, A and p-carotene, GSH and activity of GSH-Px in the supramammary lymph nodes and mammary glands of cattle.

Keywords:

lymph nodes, retinol, vitamin E, Brucella abortus, antioxidants, beta-carotene, brucellosis, cattle, defence mechanisms, enzyme activity, glutathione peroxidase, immune response, lipid peroxidation, mammary glands,

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