MİNE ERİŞİR, Mustafa TAMSER, Bülent TAŞDEMİR, Mehmet ÇAY

Ovariektomili ratlara estradiol 17-Beta ve vitamin E verilmesinin arginaz aktivitesi üzerine etkisi

Bu çalışmada normal ve diabetik ratlarda ovariektominin karaciğer ve böbrek arginazı (L-arginin amidinohidrolaz, EC 3.5.3.1) üzerine etkisi ve tüm ovariektomili ratlara estradiol ve vitamin E ilavesinin arginaz aktivitesi üzerine koruyucu etkisinin olup olmadığı araştırılmıştır. 70 adet Wistar Albino türü dişi rat 7 gruba bölünmüştür. 1) kontrol grubu, 2) Ovariektomi grubu, 3) Ovariektomi yapılmış ve Estradiol 17-P uygulanmış grup 4) Ovariektomi yapılmış, Estradiol 17-P ve vitamin E uygulanmış grup, 5) Ovariektomi yapılmış, diabetik grub (Ovariektomize edilmiş ratlara 45 mg/kg tek doz streptozotosin intraperitoneal olarak uygulanmıştır), 6) Ovariektomi yapılmış, diabetik ve Estradiol 17-J3 uygulanmış grup. 7) Ovariektomi yapılmış, diabetik, Estradiol 17-p ve E vitamini uygulanmış grup. Ratlara Estradiol 17-p (E2) 40 //g/kg/gün dozunda deri altı ve E vitamini (dl-a-tocopheryl acetate) 100 mg/kg/gün dozunda periton içi 28 gün süre ile uygulanmıştır. Ovariektomi karaciğer arginaz aktivitesini değiştirmemiş fakat böbrek arginaz aktivitesinin önemli (P

Anahtar Kelimeler:

arginaz, diyabet, enzim aktivitesi, östradiol, glutamat, böbrekler, karaciğer, östrojen, ovariektomi, poliaminler, prolin, sıçan, E vitamini

Effect of estradiol 17-Beta and vitamin E administration on arginase activity in ovariectomized rats

This study was carried out to observe the effect of ovariectomy on arginase (L-arginine amidinohydrolase, EC 3.5.3.1) activities of liver and kidney in normal and diabetic rats and to find out if there is a protective effect of estrogen and vitamin E administration on arginase activity in ovariectomized rats. Seventy Wistar albino rats were divided into seven groups as follows: 1) Control group, 2) Ovariectomy group, 3) Ovariectomized and Estradiol 17-p-treated group, 4) Ovariectomized, Estradiol 17-p- and vitamin E-treated group, 5) Ovariectomized, diabetic group (Streptozotocin to ovariectomized rats was intraperitoneally (IP) administered at a dose of 45 mg/kg body weight), 6) Ovariectomized, diabetic and Estradiol 17-P-treated group 7) Ovariectomized, diabetic, Estradiol 17-p- and vitamin E- treated group. Estradiol 17-p (E2) was administered subcutan at a dose of 40 /<g/kg, vitamin E (dl-a-tocopheryl acetate) was done intra periton at a dose of 100 mg/kg over 24 h for 28 days. Liver arginase activity was not affected by ovariectomy, but kidney arginase activity significantly decreased (P<0.001). Estradiol 17-p- and vitamin E- administration of ovariectomized rats did not change the activities. Liver arginase activity of ovariectomized diabetic rats was found to be significantly increased (P<0.001). In the ovariektomized diabetic group the arginase activity increased significantly (P<0.001) and by the administration of Estradiol 17-P alone in ovariektomized diabetic rats, arginase activity in the liver did not change, whereas administration of Estradiol 17-P and vitamin E combination made the activity level to turn to normal levels. In the ovariectomized diabetic group kidney arginase activity did not change whereas adminisration of Estradiol 17-P increased the arginase activity significantly (P<0.001). We suggest that proline, glutamate, and polyamine synthesis in kidneys may decrease by lowering kidney arginase activity in ovariectomy and that may cause to begin the kidney problems.

Keywords:

arginase, diabetes, enzyme activity, estradiol, glutamates, kidneys, liver, oestrogens, ovariectomy, polyamines, proline, rats, vitamin E,

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