Zia Ur REHMAN, Qing LIU, Dinesh BHATTARAI, Aixin LIANG, Farman ULLAH, Rahim DAD, Sher KHAN, Hira Sajjad TALPUR, Sher Ali KHAN, Liguo YANG

Expression Profile of Sox5 and Sox6 in Sertoli and Spermatogonial Cells in Growing Mice Testis

SRY box genes are peculiar to animal kingdom. They are involved in many processes particularly sex determination and testes development in male embryo. Although Sox family genes have been identified in various cells, their expression pattern and role is not entirely recognized in Sertoli cells. In this research, we focused on the expression of SoxD group Sox5 and Sox6 genes in Sertoli cells of mice during pre- and postpubertal testicular development ranging from one-week-old to eight-week-old mice. The expression was studied by immunohistochemistry on whole testes, and qPCR to determine the mRNA level of all age groups, while immunocyto-chemistry was performed for localization in specific age groups. qPCR results of Sertoli cells from first week to eight week showed diffrent levels of expression. The mRNA level of Sox5, during prepubertal age, was significantly high (P

Gelişme Dönemindeki Fare Testislerinde Sertoli Hücreleri ve Spermatogonial Hücrelerde Sox5 ve Sox6 Ekspresyonu

SRY gen grubu hayvanlar alemine özgüdür. Erkek embriyoda cinsiyet tayini ve testis gelişimi başta olmak üzere birçok sürece dahildirler. Sox familyası genleri çeşitli hücrelerde belirlenmiş olsa da, Sertoli hücrelerindeki ekspresyon özellikleri ve rolleri tam olarak tanımlanmamıştır. Bu araştırmada, puberta öncesi ve sonrası bir ila sekiz haftalık yaştaki farelerde testis gelişimi sırasında Sertoli hücrelerindeki SoxD grubu Sox5 ve Sox6 genlerinin ekspresyonu araştırıldı. Ekspresyon, tüm testis örneklerinde immünohistokimya ve tüm yaş gruplarının mRNA seviyesini belirlemek için qPCR ile incelenirken, spesifik yaş gruplarında lokalizasyonun belirlenmesi için immünositokimya yapıldı. Sertoli hücrelerinin qPCR sonuçlarında, ilk haftadan sekizinci haftaya kadar farklı ekspresyon seviyeleri gözlendi. Puberta öncesi yaşlarda Sox5’in mRNA seviyesi anlamlı derecede yüksekti (P

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