Mochamad LAZUARDI, Suharjono SUHARJOMO, Chi-Hsien CHIEN, Jie-Long HE, Chia-Kang PENG, Chi-Wen LEE, Bambang HERMANTO, Mohammad SUKMANADI, Rahmi SUGIHARTUTI, Lilik MASLACHAH

Progesteron-Benzeri Bileşiklerin %10 Lipozom İçerisinde Kapsüllenmesi ve Bu Bileşiklerin Enjektabl Dozaj Formunun Uygulandığı Sıçanlardaki Konsantrasyonlarını ArtırıR

Sentetik tıptaki boşluğu doldurmak için bitkisel ilaçların kullanılması sağlıklı yaşam tarzları için oldukça gereklidir. Benalu Duku (BD) alt türü olan Dendrophthoe pentandra L. Miq’in yaprak ekstraklarından elde edilen progesteron benzeri bileşiklerin (PLC), anti-kanserojenik ve androjenik maddelerden oluşan faydalı bileşikler içerdiği bilinmektedir. Aktif bileşiklerin yavaş salınımı, yavaş salınan araçlar kullanılarak elde edilebilir. Küçük tek katmanlı veziküller (SUV) olan lipozomlar, biyoaktif maddelerin zamanında salınımını sağlamak için güvenilir bir şekilde kullanılabilen eksipiyan maddelerden biridir. Bu çalışma, PLC’lerin %10 lipozomal SUV içinde kapsüllenmesinin biyoaktif bileşiklerin kademeli olarak salınmasını sağladığını göstermek için yapılmıştır. 100 g vücut ağırlığı başına üç farklı doz (3; 5; 7 mg) PLC-Lipozom SUV deneme gruplarındaki ratlara (15 rat) enjekte edildi. Daha sonra PLC’lerin plazma konsantrasyonları, sıvı kromatografi-elektrosprey iyonizasyon kütle spektrometrisi (LC-ESI MS) kullanılarak değerlendirildi. Deneme gruplarındaki PLC konsantrasyonlarının, kontrol grubundakilerden 1.20 ile 2.40 kat daha yüksek olduğu saptandı. Bu bulgular, %10’luk lipozomlarda kapsüllenen ilaçların, ilaç kapsüllenmesi olmadan elde edilene göre kanda daha yüksek bir ilaç seviyesi ile sonuçlanabileceğini göstermektedir (P

Encapsulation of Progesterone-Like Compounds in 10% Liposome Increases Their Concentration in Rats Administered an Injectable Dosage Form of These Compounds

The use of herbal medicine to fill the void in synthetic medicine is very necessary for health lifestyle. Progesterone-like compounds (PLCs) from the extract of the leaves of Dendrophthoe pentandra L. Miq from the subspecies, Benalu Duku (BD), are known to contain beneficial compounds that contain anti-cancer and androgenic substances. The slow release of active compounds can be achieved using slow release vehicles. Liposomes that are small unilamellar vesicles (SUVs) are one of the excipient substances that can be reliably used as a vehicle to achieve timely release of bioactive substances. This study was conducted to demonstrate that encapsulating PLCs in 10% liposomal SUV enables the gradual release of bioactive compounds. Three single doses of 3; 5; 7 mg PLCs-Liposome SUV/100 g per body weight of rats were injected into rats in the trial groups (15 of rats). Thereafter, the plasma concentrations of PLCs were assessed using liquid chromatography electrospray ionization mass spectrometry (LC-ESI MS). The concentrations of PLCs in the trial groups were found to be 1.20 to 2.40 fold higher than those in the control group. Such findings indicate that encapsulating drugs in 10% liposomes can result in a higher drug level in blood than that obtained without drug encapsulation (P

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1. Hardiyanti R, Marpaung L, Adnyana IK, Simanjunta P: Antioxidant and antibacterial activities of various extracts of duku’s mistletoe leaf (Dendrophthoe Pentandra (L.) Miq) collected from medan, indonesia. Asian J Pharm Clin Res, 11, 526-529, 2018. DOI: 10.22159/ajpcr.2018.v11i12.29725
2. Risvanli A, Ocal H, Timurkaan N, Ipek P, Seker I, Karabulut B: Expression of the anti-Mullerian hormone, kisspeptin 1, and kisspeptin 1 receptor in polycystic ovary syndrome and controlled ovarian stimulation rat models. Bosn J Basic Med Sci, 20 (1): 37-43, 2020. DOI: 10.17305/ bjbms.2019.4281
3. Maher T, Ahmad Raus R, Daddiouaissa D, Ahmad F, Adzhar NS, Latif ES, Abdulhafiz F, Mohammed A: Medicinal plants with anti- leukemic effects: A review. Molecules, 26 (9): 2741, 2021. DOI: 10.3390/ molecules26092741
4. Domínguez-López I, Yago-Aragón M, Salas-Huetos A, Tresserra- Rimbau A, Hurtado-Barroso S: Effects of dietary phytoestrogens on hormones throughout a human lifespan: A review. Nutrients, 12 (8): 2456, 2020. DOI: 10.3390/nu12082456
5. Watcho P, Meli Watio H, Wankeu-Nya M, Ngadjui E, Deeh Defo P, Nkeng-Efouet PA, Nguelefack TB, Kamanyi A: Androgenic effects of aqueous and methanolic extracts of Ficus asperifolia in male Wistar rats. BMC Complement Altern Med, 17 (1): 42, 2017. DOI: 10.1186/s12906-016- 1547-5
6. Ștefănescu R, Tero-Vescan A, Negroiu A, Aurică E, Vari CE: A comprehensive review of the phytochemical, pharmacological, and toxicological properties of Tribulus terrestris L. Biomolecules, 10 (5): 752, 2020. DOI: 10.3390/biom10050752
7. Yuen F, Thirumalai A, Pham C, Swerdloff RS, Anawalt BD, Liu PY, Amory JK, Bremner WJ, Dart C, Wu H, Hull L, Blithe DL, Long J, Wang C, Page ST: Daily oral administration of the novel androgen 11β-MNTDC markedly suppresses serum gonadotropins in healthy men. J Clin Endocrinol Metab, 105 (3): e835-e847, 2020. DOI: 10.1210/clinem/dgaa032
8. Elghandour MMMY, Reddy PRK, Salem AZM, Reddy PPR, Hyder I, Barbabosa-Pliego A, Yasaswini D: Plant bioactives and extracts as feed additives in horse nutrition. J Equine Vet Sci, 69, 66-77, 2018. DOI: 10.1016/j.jevs.2018.06.004
9. Ciolacu DE, Nicu R, Ciolacu F: Cellulose-based hydrogels as sustained drug-delivery systems. Materials (Basel), 13 (22): 5270, 2020. DOI: 10.3390/ ma13225270
10. Sforzi J, Palagi L, Aime S: Liposome-based bioassays. Biology (Basel), 9 (8): 202, 2020. DOI: 10.3390/biology9080202
11. Ciesielska A, Matyjek M, Kwiatkowska K: TLR4 and CD14 trafficking and its influence on LPS-induced pro-inflammatory signaling. Cell Mol Life Sci, 78 (4): 1233-1261, 2021. DOI: 10.1007/s00018-020-03656-y
12. Witika BA, Makoni PA, Matafwali SK, Chabalenge B, Mwila C, Kalungia AC, Nkanga CI, Bapolisi AM, Walker RB: Biocompatibility of biomaterials for nanoencapsulation: Current approaches. Nanomaterials (Basel), 10 (9): 1649, 2020. DOI: 10.3390/nano10091649
13. Aguilar-Pérez KM, Medina DI, Narayanan J, Parra-Saldívar R, Iqbal HMN: Synthesis and nano-sized characterization of bioactive oregano essential oil molecule-loaded small unilamellar nanoliposomes with antifungal potentialities. Molecules, 26 (10): 2880, 2021. DOI: 10.3390/ molecules26102880
14. Foley K, Gupta AK, Versteeg S, Mays R, Villanueva E, John D: Topical and device-based treatments for fungal infections of the toenails. Cochrane Database Syst Rev, 1 (1): CD012093, 2020. DOI: 10.1002/ 14651858.CD012093.pub2
15. Patra JK, Das G, Fraceto LF, Campos EVR, Rodriguez-Torres MDP Acosta-Torres LS, Diaz-Torres LA, Grillo R, Swamy MK, Sharma S, Habtemariam S, Shin HS: Nano based drug delivery systems: recent developments and future prospects. J Nanobiotechnol, 16 (1): 71, 2018. DOI: 10.1186/s12951-018-0392-8
16. Woo SW, Jo YK, Yoo YE, Kim SK: High-throughput synthesis of liposome using an injection-molded plastic micro-fluidic device. Micromachines (Basel), 12 (2): 170, 2021. DOI: 10.3390/mi12020170
17. Scriboni AB, Couto VM, Ribeiro LNM, Freires IA, Groppo FC, de Paula E, Franz-Montan M, Cogo-Müller K: Fusogenic liposomes increase the antimicrobial activity of vancomycin against Staphylococcus aureus biofilm. Front Pharmacol, 10:1401, 2019. DOI: 10.3389/fphar.2019.01401
18. Lazuardi M, Hermanto B, Hestianah EP: Determination of progesterone compounds in the crude methanol extract of benalu duku leaves. Vet World, 12 (3): 358-366, 2019. DOI: 10.14202/vetworld.2019.358-366
19. Cortese M, Gigliobianco MR, Magnoni F, Censi R, Di Martino PD: Compensate for or minimize matrix effects? Strategies for overcoming matrix effects in liquid chromatography-mass spectrometry technique: A tutorial review. Molecules, 25 (13): 3047, 2020. DOI: 10.3390/ molecules25133047
20. Taherdoost H: Sampling methods in research methodology: How to choose a sampling technique for research. Int J Acad Res Manag, 5 (2): 18- 27, 2016. DOI: 10.2139/ssrn.3205035
21. Putri DCA, Dwiastuti R, Marchaban M, Nugroho AK: Optimization of mixing temperature and sonication duration in liposome preparation. J Pharm Sci Commun, 14 (2): 79-85, 2017. DOI: 10.24071/jpsc.142728
22. Susakate S, Poapolathep S, Chokejaroenrat C, Tanhan P, Hajslova J, Giorgi M, Saimek K, Zhang Z, Poapolathep A: Multiclass analysis of antimicrobial drugs in shrimp muscle by ultra high performance liquid chromatography-tandem mass spectrometry. J Food Drug Anal, 27 (1): 118-134, 2019. DOI: 10.1016/j.jfda.2018.06.003
23. Suh JH, Makarova AM, Gomez JM, Paul LA, Saba JD: An LC/MS/ MS method for quantitation of chemopreventive sphingadienes in food products and biological samples. J Chromatogr B Analyt Technol Biomed Life Sci, 1061-1062, 292-299, 2017. DOI: 10.1016/j.jchromb.2017.07.040
24. Malysheva SV, Mulder PPJ, Masquelier J: Development and validation of a UHPLC-ESI-MS/MS method for quantification of oleandrin and other cardiac glycosides and evaluation of their levels in herbs and spices from the Belgian market. Toxins (Basel), 12 (4): 243, 2020. DOI: 10.3390/toxins12040243
25. Srivastava D, Fatima Z, Kaur CD, Tulsankar SL, Nashik SS, Rizvi DA: Pharmaceutical cocrystal: A novel approach to tailor the biopharmaceutical properties of a poorly water-soluble drug. Recent Pat Drug Deliv Formul, 13 (1): 62-69, 2019. DOI: 10.2174/1872211313666190 306160116
26. Trucillo P, Campardelli R, Reverchon E: Liposomes: From bangham to supercritical fluids. Processes, 8 (9): 1022, 2020. DOI: 10.3390/pr809102