MEHMET BOZKURT ATAMAN, HÜSEYİN ERDEM, Bülent BÜLBÜL, SEYFULLAH HALİLOĞLU, MİYASE ÇINAR, MEHMET AKÖZ

Plasma β-carotene, vitamin A and vitamin C levels in cyclic and pregnant cows

Bu çalışmanın amacı siklik ve gebe ineklerde plazma β-karoten, vitamin A ve vitamin C seviyelerini ve bu değerler arasındaki ilişkiyi belirlemekti. Bu amaçla toplam 101 Holstein inek kullanıldı. Östrüs tespitinden 12 saat sonra ineklere suni tohumlama uygulandı. İnekler gebeliklerinin belirlenmesinden sonra gebe (n=81) ve gebe olmayan (n=20) olarak iki gruba ayrıldı. Progesteron seviyesi yalnız 21. günde gebe ineklerde gebe olmayan ineklerden yüksek bulundu (P

Anahtar Kelimeler:

kızgınlık dönemi, retinol, progesteron, gebelik, kızgınlık, süt ineği, süt sığırı, inek, sığır, kan plazması, kan kimyası, beta-karoten, askorbik asit, suni tohumlama

Siklik ve gebe ineklerde plasma β-karoten, vitamin A ve vitamin C seviyeleri

The aim of this study was to determine the plasma β-carotene, vitamin A and vitamin C levels and correlation between these parameters in cyclic and pregnant dairy cattle. A total of 101 Holstein cows used for this aim. Artificial inseminations (AI) were performed 12 h after determining the oestrus. The cows were allocated to two groups as pregnant (n=81) and nonpregnant (n=20) after the determination of pregnancy. Progesterone level was only higher on day 21 in pregnant cows than that in nonpregnant cows (P<0.05). There were variations in the mean levels of β-carotene and vitamin A in pregnant and nonpregnant cows (P<0.05) whereas vitamin C levels in nonpregnant cows did not differ during the oestrus cycle. There was a negative correlation between the plasma levels of progesterone and β-carotene (P<0.01) and progesterone and vitamin A (P<0.05) in pregnant cows, and a positive correlation between the plasma levels of β-carotene and vitamin A in both pregnant and nonpregnant cows (P<0.01), during the cycle. In conclusion, differences were determined in the levels of β-carotene, vitamin A and vitamin C in pregnant cows, and b-carotene and vitamin A in nonpregnant cows with the stages of the oestrus cycle in this study. In addition to this, more research is needed evaluating the relationship between these parameters and their effects on bovine reproduction.

Keywords:

oestrous cycle, retinol, progesterone, pregnancy, oestrus, dairy cows, dairy cattle, cows, cattle, blood plasma, blood chemistry, beta-carotene, ascorbic acid, artificial insemination,

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1.Santos JEP, Bartolome JA, Cerri RLA, Juchem SO, Hernandez O, Trigg T, Thatcher WW: Effect of a deslorelin implant in a timed artificial insemination protocol on follicle development, luteal function and reproductive performance of lactating dairy cows. Theriogenology, 61, 421-435, 2004.
2.Vasconcelos JL, Deme´trio DGB, Santos RM, Chiari JR, Rodrigues CA, Sa´ Filho OG: Factors potentially affecting fertility of lactating dairy cow recipients. Theriogenology, 65, 192-200, 2006.
3.Aslan S, Handler J, Arbeiter K: Frühgravidität und embryonale bzw. frühfetale Mortalität bei der Kuh-Gelbkörperdynamik, Progesteron-, Vitamin-E-, Vitamin B12-, β-Carotin- und Folsäurekonzentrationen im peripheren Blut. Wien Tierarztl Mschr, 85, 141-147, 1998.
4.Graves-Hoagland RL, Hoagland TA, Woody CO: Effect of βcarotene and vitamin A on progesterone production by bovine luteal cells. J Dairy Sci, 71, 1058-1062, 1988.
5.Haliloğlu S, Baspmnar N, Serpek B, Erdem H, Bulut Z: Vitamin A and β-carotene levels in plasma, corpus luteum and follicular fluid of cyclic and pregnant cattle. Reprod Dom Anim, 37, 96-99, 2002.
6.Hemken RW, Bremel DH: Possible role of beta-carotene in improving fertility in dairy cattle. J Dairy Sci, 65, 1069-1073, 1982.
7.Ceylan A, Serin İ, Akşit H, Seyrek K, Gokbulut C: Döl tutmayan ve anöstruslu süt ineklerinde vitamin A, E, betakaroten, kolesterol ve trigliserid düzeylerinin araştdrdlmasd. Kafkas Univ Vet Fak Derg, 13, 143-147, 2007.
8.Talavera F, Chew BP: Comparative role of retinol, retinoic acid and β-carotene on progesterone secretion by pig corpus luteum in vitro. J Reprod Fert, 82, 611-615, 1988.
9.Kaçar C, Kamiloğlu NN, Uçar Ö, Arı UÇ, Pancarcı ŞM, Güngör Ö: İneklerde β-karoten+E vitamini uygulamasdyla kombine edilen Ovsynch ve Cosynch senkronizasyon programlarının gebelik orand üzerine etkisi. Kafkas Univ Vet Fak Derg, 14, 45-50, 2008.
10.Arıkan Ş, Rodway RG: Seasonal variation in bovine luteal concentrations of β-carotene. Tr J Vet Anim Sci, 25, 165-168, 2001.
11.Tharnish T, Larson LL: Vitamin a suplementation of Holsteins at high concentrations: Progesterone and reproductive responses. J Dairy Sci, 75, 2374-2381, 1992.
12.Çelik HA, Avcı G, Aydın İ, Bülbül A, Bülbül T: Effect of βcarotene on ovarium functions and Ovsynch success in repeat breeder cows. Kafkas Univ Vet Fak Derg, 15, 87-94, 2009.
13.Arıkan Ş, Muğlalı ÖH: Bazı çiftlik hayvanlarının üreme fonksiyonlard üzerine β-karotenin etkisi. Lalahan Hay Araşt Enst Derg, 39, 85-94, 1999.
14.Pethes BG, Horváth E, Kulcsár M, Huszenicza G, Somorjai G, Varga B, Haraszti J: In vitro progesterone production of corpus luteum cells of cows fed low and high levels of beta-carotene. Zbl Vet Med A, 32, 289-296, 1985.
15.Jukola E, Hakkarainen J, Saloniemi, Sankari S: Blood selenium, vitamin E, vitamin A, and β-carotene concentrations and udder health, fertility treatments, and fertility. J Dairy Sci, 79, 838-845, 1996.
16.Aréchiga CF, Vázquez-Flores S, Ortiz O, Hernández-Cerón J, Porras A, McDowell LR, Hansen JP: Effect of injection of β-carotene or vitamin E and selenium on fertility of lactating dairy cows. Theriogenology, 50, 65-76, 1998.
17.Gossen N, Feldmann M, Hoedemaker M: Effect of parenteral supplementation with beta-carotene in the form of an injection solution (Carofertin) on the fertility performance of dairy cows. Dtsch Tierarztl Wochenschr, 111, 14-21, 2004.
18.Schweigert FJ, Zucker H: Concentrations of vitamin A, βcarotene and vitamin E in individual bovine follicles of different quality. J Reprod Fert, 82, 575-579, 1988.
19.Weiss WP: Requirements of fat-soluble vitamins for dairy cows: A review. J Dairy Sci, 81, 2493-2501, 1998.
20.Brown BS, Whisnant CS, Reed BK: Effect of vitamin A injection on the response to superovulation in beef cattle. J Anim Sci, 75, 211, 1997.
21.Haliloğlu S, Serpek B: Koyunlarda plazma vitamin C ve seruloplazmin düzeyleriyle eksojen vitamin C uygulamalardndn döl verimi üzerine etkileri. Tr J Vet Anim Sci, 24, 403-411, 2000.
22.Weiss WP: Effect of dietary vitamin C on concentrations of ascorbic acid in plasma and milk. J Dairy Sci, 84, 2302-2307, 2001.
23.Hıdıroglou M: Technical note: forms and route of vitamin C supplementation for cows. J Dairy Sci, 82, 1831-1833, 1999.
24.Luck MR, Zhao Y: Identification and measurement of collagen in the bovine corpus luteum and relationship with ascorbic acid and tissue development. J Reprod Fert, 99, 647-652, 1993.
25.Haliloğlu S, Serpek B, Baspinar N, Erdem H, Bulut Z: Holstein drkd gebe ineklerin plazma ve ovaryum vitamin C, progesteron ve 17β-östradiol düzeyleri arasdndaki ilişkiler. Tr J Vet Anim Sci, 26, 639-644, 2002.
26. Miszkiel G, Skarzynski D, Bogacki M, Kotwica J: Concentrations of catecholamines, ascorbic acid, progesterone and oxytocin in the corpora lutea of cyclic and pregnant cattle. Reprod Nutr Dev, 39, 509-516, 1999.
27.Serpek B, Baspınar N, Halıloglu S, Erdem H: The relationship between ascorbic acid, oestradiol 17β and progesterone in plasma and in ovaries during the sexual cycle in cattle. Revue Méd Vét, 152, 253-260, 2001.
28.Yıldız H, Kaygusuzoğlu E, Kızıl Ö: Serum progesterone, vitamin A, E, C and β-carotene levels in pregnant and nonpregnant cows post-mating. J Anim Vet Adv, 4, 381-384, 2005.
29.Parakash BS, Meyer HHD, Schallenberger E, Van de Wiel DFM: Development of a sensitive enzyme immuno-assay (EIA) for progesterone determination in unexracted bovine plasma using the second antibody technique. J Steroid Biochem, 28, 623-627, 1987.
30.Suzuki J, Katoh N: A simple and cheap method for measuring serum vitamin A in cattle using only a spectrophotometer. Jpn J Vet Sci, 52, 1281-1283, 1990.
31.Haag W: Zur methodik und praktischen Bedeutung der Vitamin C-Bestimmung beim Rind in Vergangenheit und Gegenwart. Inaugural Dissertation. Justus Liebig Universitaet, Giessen, 1985.
32.Graves-Hoagland RL, Hoagland TA, Woody CO: Relationship of plasma β-carotene and vitamin A to luteal function in postpartum cattle. J Dairy Sci, 72, 1854-1858, 1989.
33.Aréchiga CF, Staples CR, McDowell LR, Hansen JP: Effects of timed insemination and supplemental β-carotene on reproduction and milk yield of dairy cows under heat stress. J Dairy Sci, 81, 390-402, 1998.
34.Rapaport R, Sklan D, Wolfenson D, Shaham-Albalancy A, Hanukoglu I: Antioxidant capacity is correlated with steroidogenic status of the corpus luteum during the bovine oestrus cycle. Biochim Biophys Acta, 1380, 133-140, 1998.
35.Iwańska S, Strusińska D: The effect of beta-carotene and vitamins A, D3 and E on some reproductive parameters in cows. Acta Vet Hung, 45, 95-107, 1997.
36.Akar Y, Gazioglu A: Relationship between vitamin A and β-carotene levels during the postpartum period and fertility parameters in cows with and without retained placenta. Bull Vet Inst Pulawy, 50, 93-96, 2006.
37.Gossen N, Hoedemaker M: Effect of beta-carotin serum concentration on the reproductive performance in dairy cows. Berl Munch Tierarztl Wochenschr, 118, 326-33, 2005.
38.Lotthammer KH: Importance of beta-carotene for the fertility of dairy cattle. Feedstuffs, 51, 16-38, 1979.
39.Johnston LA, Chew BP: Peripartum changes of plasma and milk vitamin A and beta-carotene among dairy cows with or without mastitis. J Dairy Sci, 67, 1832-1840, 1984.
40.Fujitani Y, Kasai K, Ohtani S, Nishimura K, Yamada M, Utsumi K: Effect of oxygen concentration and free radicals on in vitro development of in vitro-produced bovine embryos. J Anim Sci, 75, 483-489, 1997.
41.Gordon IR: Reproductive technologies in farm animals. CAB International, Cambridge, 2005.
42.Gordon IR: Laboratory production of cattle embryos. 2nd ed., CAB International, Cambridge, 2003.
43.Luck MR, Jeyaseelan E, Scholas RA: Ascorbic acid and fertility. Biol Reprod, 52, 262-266, 1995.
44.Hansen PJ, Drost M, Rivera RM, Paula-Lopes FF, Al-Katanani YM, Krininger CE, Chase CC: Adverse impact of heat stres on embryo production: Causes and strategies for mitigation. Theriogenology, 55, 91-103, 2001.