Özgür ÖZDEMİR, Mustafa ORTATATLI, Funda TERZİ, Fatih HATİPOĞLU, Mustafa Kemal ÇİFTÇİ, MEHMET BURAK ATEŞ

The Usability of Cytological and Immunocytological Methods for Rapid Diagnosis of Encephalitic Listeriosis in Ruminants

Although the clinical and pathological findings are important in the diagnosis of listeriosis, to isolation or to be shown the presence of the bacterium must be required for the definitive diagnosis. This study aims to investigate the availability of imprint cytological (IC) and immunocytochemical (ICC) methods in comparison with histopathological and immunohistochemical (IHC) methods for the rapid diagnosis of encephalitic listeriosis . In the study, the touching and smear preparations taken from the pons and medulla oblongata of 25 ruminants suspected with listeriosis by neurological symptoms were stained with modified giemsa and also with ICC technic for revealing antigens, as a new method. Same tissue sections were stained with Hematoxylin&Eosin and IHC methods too, and examined under light microscope by scoring. In IC examinations, there were intensive neutrophils in 14 cases and few neutrophils in 4 cases, and no neutrophils were observed in 7 cases. In histopathological examinations, 13 of these 14 cases revealed typical microabscesses and listeria positivity in IHC staining. ICC positivity was detected in 12 (92.3%) of the listeria positive 13 cases. A highly positive correlation was observed among cytology (14), ICC (12), histopathology and IHC (13) scores (r2> 0.8; P

Ruminantlarda Ensefalitik Listeriozisin Hızlı Tanısı İçin Sitolojik ve İmmunositolojik Yöntemlerin Kullanılabilirliğinin Araştırılması

Listeriozisin tanısında klinik ve patolojik bulgular önemli ise de, kesin teşhiste etken izolasyonu veya etkenin varlığının gösterilmesi gerekmektedir. Bu çalışmada ruminantlarda ensefalitik listeriozisin hızlı teşhisinde sitolojik ve immunositokimyasal (ICC) yöntemlerin, histopatoloji ve immunohistokimyasal (IHC) yöntemle kıyaslanarak rutinde kullanılabilirliklerinin araştırılması amaçlandı. Çalışmada, sinirsel semptomlarla listeriozisten şüphelenilen 25 adet ruminantın (1 sığır, 1 buzağı, 11 koyun, 9 kuzu, 1 keçi ve 2 oğlak) pons ve medulla oblongatasından alınan sürme ve kazıntı preparatlar modifiye Giemsa ile ve ayrıca ICC yöntemle boyandı. Yine aynı bölümlerden alınan doku örnekleri Hematoksilen-Eozin ve IHC yöntemleriyle de boyandı ve skorlanarak ışık mikroskobunda incelendi. Sitolojik muayenelerde 14 olguda yoğun, 4 olguda ise az sayıda nötrofil görülürken, 7 olguda hiç nötrofil gözlenmedi. Sitolojik olarak listeriozis olduğu değerlendirilen bu 14 vakanın 13’ünde histopatolojik incelemelerde tipik mikroapse ve perivaskuler hücre infiltrasyonu ile IHC boyamalarında listeria pozitifl iği bulundu. IHC yöntemi baz alındığında, listeria pozitif 13 vakanın 12’sinde ICC pozitifl iği (%92.3) belirlendi. Sitolojide az sayıda nötrofil görülen 4 olgu ile nötrofil görülmeyen 7 olguda ise mikroapseye rastlanmadı, IHC ve ICC de negatif olarak bulundu. Sitoloji (14), ICC (12) ile histopatoloji ve IHC (13) skorları arasında yüksek oranda (r2>0.8; P

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