Midye ve Karideslerde Rotavirus, Adenovirus ve Astrovirus Varlığının Multiplex Real-time PCR Kullanılarak Araştırılması

Viruslar gıda vasıtasıyla en yaygın bulaşan patojenlerden biridir. Epidemiyolojik bulgulara göre insana ait enterik viruslar kabuklu deniz ürünleri vasıtasıyla en çok aktarılan etiyolojik ajan olarak tanımlanmaktadır. İstanbul’da balıktan sonra en çok tüketilen deniz ürünleri midye ve karidestir. Bu çalışmada, İstanbul kıyılarında avlanarak tüketilen midye ve karides numunelerinde rotavirus, adenovirus ve astrovirus varlığı araştırılmıştır. Bu amaçla toplam 28 karides ve 52 midye grubu İstanbul’un farklı yerlerinden toplanmıştır. Her bir grup ayrı bir numune olarak analize alınmıştır. Viruslar glisin/NaCl, pH 9.5 tamponu kullanarak direkt elüsyon metodu ile doğrudan sindirim dokusundan ayrıştırılmış ve PEG-6000 ile konsantre edilmiştir. Alınan midye ve karides numuneleri Multiplex Real-time PCR yöntemi ile analiz edilmiştir. Toplam çalışılan 52 midye numunesinin %46.15’inde adenovirus ve %63.46’sında astrovirus saptanmıştır. Rotavirus hiçbir midye numunesinde tespit edilmemiştir. Karides numunelerinin hiçbirinde araştırılan virusların hiçbiri saptanmamıştır. Bu, Türkiye’deki midyelerde ve karideslerde rotavirus, adenovirus ve astrovirus prevalansı hakkındaki ilk rapordur. Sonuçlar, İstanbul’da satılan midyelerin adenovirus ve astrovirus ile kontamine olduğunu ve bu durumun halk sağlığı için büyük bir tehdit oluşturabileceğini göstermektedir

Investigation of Rotavirus, Adenovirus and Astrovirus in Mussels and Shrimps Using Multiplex Real-time PCR

Viruses are one of the most common pathogens transmitted via food. Based on epidemiological evidence, human enteric viruses areconsidered to be as the most commonly transmitted etiological agents by bivalve shellfish. In Istanbul, the most widely consumed seaproducts after fish are mussels and shrimps. In this study, the presence of rotavirus, adenovirus and astrovirus were investigated in musseland shrimp samples consumed by hunting on the shores of Istanbul. For this purpose, a total of 28 groups of shrimp and 52 groups of musselwere collected from different places in Istanbul. Each group was analyzed separately as a sample. Viruses were extracted from the digestivetissue by direct elusion method in a glycine/NaCl, pH 9.5 buffer followed by PEG-6000 precipitation. Multiplex Real-time RT-PCR techniquewas used to analyze the shellfish samples. Astrovirus was found in 63.46% of the total 52 analyzed mussel samples, whereas adenovirus wasfound in 46.15%. None of the mussel samples was positive for rotavirus. All tested shrimp samples were negative for all three researchedviruses. This is the first report on the prevalence of rotavirus, adenovirus and astrovirus in mussels and shrimps in Turkey. The results showthat mussels sold in Istanbul are highly contaminated with adenovirus and astrovirus and this could pose a great threat to public health.

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