This study aimed to investigate the prevalence of Salmonella spp. and Listeria monocytogenes, their antimicrobial resistance profile. L. monocytogenes was not isolated from any of the samples. Salmonella spp. was detected from 32 (8%) out of the 400 collected samples. Antimicrobial resistance was most frequently observed to nalidixic acid (100%), tetracycline (93.75%), erythromycin (90.6%), streptomycin (84.3%), followed by kanamycin (62.5%). Also, 37.5% of Salmonella isolates were phenotypically confirmed as ESBL producers. Multiple drug resistance was defined 93.75% of the isolates. Among the Salmonella isolates, all of them harbouring qnrB and qnrS genes and, 37.5% of them presented blaTEM gene..
Bu çalışmada Listeria monocytogenes ve Salmonella spp. prevalansı ve antimikrobiyal direnç profillerinin araştırılması amaçlandı. L. monocytogenes örneklerden izole edilmedi. Salmonella spp. toplanan 400 örneğin 32 (%8)’sinden saptandı. Antimikrobiyal direnç en sık nalidiksik asit (%100), tetrasiklin (%93.75), eritromisin (%90.6), streptomisin (%84.3), ardından kanamisin (%62.5)’de belirlendi. Ayrıca, Salmonella izolatlarının %37.5’i ESBL pozitif olarak fenotipik yöntemlerle doğrulandı. Çoklu ilaç direnci, izolatların %93.75’inde tanımlandı. Salmonella izolatlarının hepsinin qnrB ve qnrS geni, %37.5’inin ise blaTEM geni taşıdığı belirlendi.
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Wu H, Xia X, Cui Y, Hu Y, Xi M, Wang X, Shı X, Wang D, Meng J, Yang B: Prevalence of extended-spectrum β-lactamase–producing Salmonella on retail chicken in six provinces and two national cities in the People’s Republic of China. J Food Prot, 76 (12): 2040-2044, 2013. DOI: 10.4315/0362-028X.JFP-13-224
Nazik H, İlktaç M, Öngen B: Prevalence of qnrA, qnrB, qnrS and aac(6’)-Ib-cr (in qnr-positive isolates) among the ESBL-positive and/or ciprofloxacin-resistant isolates in Turkey. J Chemother, 21 (2): 219-221, 2009. DOI: 10.1179/joc.2009.21.2.219
Ziech RE, Lampugnani C, Perin AP, Sereno MJ, Sfaciotte RAP, Viana C, Soares VM, Pinto JPAN, Bersot LDS: Multidrug resistance and ESBL-producing Salmonella spp. isolated from broiler processing plants. Braz J Microbiol, 47 (1): 191-195, 2016. DOI: 10.1016/j.bjm.2015.11.021
Arslan S, Eyi A: Occurrence and antimicrobial resistance profiles of Salmonella species in retail meat products. J Food Prot, 73 (9): 1613-1617, 2010. DOI: 10.4315/0362-028X-73.9.1613
Dallenne C, Da Costa A, Decré D, Favier C, Arlet G: Development of a set of multiplex PCR assays for the detection of genes encoding important β-lactamases in Enterobacteriaceae. J Antimicrob Chemother, 65 (3): 490-495, 2010. DOI: 10.1093/jac/dkp498
Saladin M, Cao VTB, Lambert T, Donay JL, Herrmann JL, OuldHocine Z, Verdet C, Delisle F, Philippon A, Arlet G: Diversity of CTX-M β-lactamases and their promoter regions from Enterobacteriaceae isolated in three Parisian hospitals. FEMS Microbiol Lett, 209, 161-168, 2002. DOI: 10.1111/j.1574-6968.2002.tb11126.x
Ciesielczuk H, Hornsey M, Choi V, Woodford N, Wareham DW: Development and evaluation of a multiplex PCR for eight plasmidmediated quinolone-resistance determinants. J Med Microbiol, 62, 1823- 1827, 2013. DOI: 10.1099/jmm.0.064428-0
CLSI: Clinical and Laboratory Standards Institute Performance standards for antimicrobial susceptibility testing: Twenty-fourth informational supplement. CLSI document M100-S24. Wayne, PA: CLSI, 2014.
Saeki EK, Alves J, Bonfante RC, Hirooka EY, Oliveira TCRM: Multiplex PCR (mPCR) for the detection of Salmonella spp. and the differentiation of the Typhimurium and Enteritidis serovars in chicken meat. J Food Safety, 33 (1): 25-29, 2013. DOI: 10.1111/jfs.12019
ISO, The International Organization of Standardizations: Microbiology of food animal feeding stuffs-horizontal method for the detection Listeria monocytogenes International Standard, ISO 11290- 2:2005.
ISO, The International Organization of Standardizations: Microbiology of food animal feeding stuffs-horizontal method for the detection Salmonella spp. International Standard, ISO 6579:2002.
Bilge N, Vatansever L, Sezer Ç: Antibiotic resistance of Salmonella spp. isolated from raw chicken wings. Kafkas Univ Vet Fak Derg, 24 (3): 431- 435, 2018. DOI: 10.9775/kvfd.2017.19134
Abd-Elghany SM, Sallam KI, Abd-Elkhalek A, Tamura T: Occurrence, genetic characterization and antimicrobial resistance of Salmonella isolated from chicken meat and giblets. Epidemiol Infect, 143 (5): 997- 1003, 2015. DOI: 10.1017/S0950268814001708
Bae DH, Dessie HK, Baek HJ, Kim SG, Lee HS, Lee YJ: Prevalence and characteristics of Salmonella spp. isolated from poultry slaughterhouses in Korea. J Vet Med Sci, 75 (9): 1193-1200, 2013. DOI: 10.1292/jvms.13-0093
Yildirim Y, Gonulalan Z, Pamuk S, Ertas N: Incidence and antibiotic resistance of Salmonella spp. on raw chicken carcasses. Food Res Int, 44 (3): 725-728, 2011. DOI: 10.1016/j.foodres.2010.12.040
Oliveira TS, Varjão LM, da Silva LNN, Pereira RDCL, Hofer E, Vallim DC, de Castro Almeida RC: Listeria monocytogenes at chicken slaughterhouse: Occurrence, genetic relationship among isolates and evaluation of antimicrobial susceptibility. Food Control, 88, 131-138, 2018. DOI: 10.1016/j.foodcont.2018.01.015
Mąka L, Maćkiw E, Ścieżyńska H, Pawłowska K, Popowska M: Antimicrobial susceptibility of Salmonella strains isolated from retail meat products in Poland between 2008 and 2012. Food Control, 36 (1): 199-204, 2014. DOI: 10.1016/j.foodcont.2013.08.025
EFSA (European Food Safety Authority): The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2016. EFSA J, 15 (12): 5077, 2017. DOI: 10.2903/j. efsa.2017.5077