Nana HU, Qiang FU, Shengwei HU, Mengting SHI, Huijun SHI, Wei NI, Jinliang SHENG, Chuangfu CHEN

Lentivirus-mediated bta-miR-193a Overexpression Promotes Apoptosis of MDBK Cells by Targeting BAX and Inhibits BVDV Replication

MikroRNAlar (MiRNA) doğal olarak bulunan, kısa, endojen, kodlama yapmayan RNA molekülleri (~22 nt) olup, hücre büyümesi, gelişmesi, farklılaşması, çoğalması ve apoptozis gibi çok çeşitli düzenleyici yolaklarda ve ayrıca viral savunmada, hematopoieziste, organ şekillenmesinde ve metabolizmada görev yapmaktadır. Yapılan çalışmalar Bovine Viral Diare Virus (BVDV)'un NADL suşu ile enfekte MadinDarby Bovine Böbrek (MDBK) hücrelerinde bta-miR-193a (miR-193a)'nın ekspresyonunun upregule edildiğini göstermiştir. Ancak, apoptozis ilişkili regulasyonda miR-193a'nın rolü bilinmemektedir. Bu çalışmada, miR-193a'nın MDBK apoptozisinde görev yapan bir regülatör olduğu ve lentivirus enfeksiyonunun miR-193a ekspresyonunda pozitif bir etki gösterdiği tespit edilmiştir. Ayrıca, miR-193a hedef sekansının B-hücre lenfoma-2-ilişkili X protein (BAX) mRNA'sının 3?-translasyon yapılmayan bölgesinde mevcut olduğu ve miR-193a overekspresyonunun azalmış BAX mRNA ve protein seviyesi ile ilişkili olduğu belirlendi. Kantitatif ters transkripsiyon polimeraz zincir reaksiyonu ile belirlendiği üzere miR193a apoptozisi artırdı ve BVDV suş NADL'nin replikasyonunu inhibe etti. Elde edilen sonuçlar miR-193a'nın apoptozisin pozitif regülatörü olduğunu onaylayarak BVDV replikasyonunun düzenlenmesinde miRNA önemli rol oynadığı hakkında teorik temel oluşturmuştur

Lentivirus Aracılı bta-miR-193a Overeksprasyonu MDBK Hücrelerinin Apoptozisini Artırır ve BVDV Replikasyonunu Baskılar

MicroRNAs (miRNAs) are a class of naturally occurring, short, endogenous, noncoding RNA molecules (~22 nt) involved in a wide variety of regulatory pathways, including cell growth, development, differentiation, proliferation, and apoptosis, as well as viral defense, hematopoiesis, organ formation, and metabolism. Previous studies showed that bta-miR-193a (miR-193a) was upregulated in Madin-Darby bovine kidney (MDBK) cells infected with bovine viral diarrhea virus (BVDV) strain NADL; however, the role of miR-193a in apoptosis-associated regulation remains unclear. In this study, we found that miR-193a is a novel regulator of MDBK apoptosis and that lentiviral infection exhibited a positive effect on miR-193a expression. Additionally, we observed that the miR-193a-target sequence was present in the 3′-untranslated region of B-cell lymphoma-2-associated X protein (BAX) mRNA, with miR-193a overexpression resulting in reduced BAX mRNA and protein levels. Furthermore, we observed that miR-193a promoted apoptosis and inhibited BVDV strain NADL replication according to quantitative reverse transcription polymerase chain reaction results. These findings confirmed miR-193a as a positive regulator of apoptosis and provided a theoretical basis for the important role of miRNAs in regulating BVDV replication

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