Masoud MOSTASHARI-MOHASES, Ali Asghar SADEGHI, Jafar AHMADI, Saeid ESMAEILKHANIAN

Effect of Betaine Supplementation on Performance Parameters, Betaine-homocysteine S-methyltransferase Gene Expression in Broiler Chickens Consume Drinking Water with Different Total Dissolved Solids

Bu çalışmanın amacı, içme suyu içerisinde değişik oranlarda toplam çözünmüş katı madde (TDS) ve betain ilavesinin broiler tavuklarda performans ve Betain-hemosistein S-metiltransferaz (BHMT) gen ekspresyonu üzerine etkisini değerlendirmektir. Tamamen rastgele olarak 3x2 faktöriyel dizayn kullanılarak, civcivler her birinde 15 hayvan ve 4 tekrar olmak üzere altı uygulama grubuna ayrıldı. Deneysel uygulamalar üç farklı dozda toplam çözünmüş katı madde (400, 2.000 ve 3.500 ppm) ve iki farklı dozda betain (diyetin %0 ve %2'si) takviyesinden oluştu. Suyun TDS'sinin artışıyla orantılı olarak kilo artışı azalırken yem dönüşüm oranı ve su tüketimi artış gösterdi. Betain ilavesi büyüme döneminde kilo artışı ve su tüketiminde etki etmezken bitirme döneminde kazanım ve yem konversiyon oranında anlamlı derece etki gösterdi. Suyun TDS'i arttıkça mortalite ve dışkı nem oranında artış tespit edildi. Dışkı nem oranı betain takviyesi ile azalma gösterdi. BHMT gen ekspresyonu TDS seviyesindeki artışla ilişkili olarak anlamlı oranda azalırken betain ilavesi betain uygulanmayan grupla karşılaştırıldığında 28 kat artış gösterdi. Sonuç olarak içme suyu içerisinde 2.000 ppm'den daha yüksek oranda TDS'in tüketilmesi broiler civcivlerde performansı olumsuz yönde etkilerken betain takviyesi pozitif yönde etki gösterdi. En düşük yem konversiyon oranı betain takviyesi ile birlikte 400 ve 2.000 ppm TDS'li civcivlerde gözlemlendi

Betain İlavesinin Değişik Miktarlarda Toplam Çözünmüş Katı Madde Tüketen Broiler Tavuklarda Performans Parametrelerine ve Betain-hemosistein S-metiltransferaz Gen Ekspresyonuna Etkisi

The objective of the current work was to evaluate the effect of drinking water with various levels of total dissolved solids (TDS) and betaine supplementation on the performance and gene expression of betaine-homocysteine S-methyltransferase (BHMT) in broiler chickens. In a completely randomized design with a 3×2 factorial arrangement, chicks were assigned to six treatments with four replicates and 15 chicks per each. The treatments were included of three levels of total dissolved solids (400, 2.000 and 3.500 ppm) and two levels of betaine supplementation (0 and 0.2% of diet). Weight gain decreased and feed conversion ratio and water intake increased as TDS of water increased. Betaine supplementation had no effect on weight gain and water intake during the grower period, but had a significant effect on gain and feed conversion ratio in the finisher period. Mortality rate and excreta moisture content increased as TDS of water increased. Excreta moisture content decreased with betaine supplementation. Gene expression of BHMT decreased significantly with increases in TDS level and betaine supplementation increased its expression (28 folds) as compared with the non-additive group. It was concluded that consumption of drinking water with higher than 2.000 ppm TDS adversely and betaine supplementation positively affect the performance of broiler chickens. The lowest feed conversion ratio was seen in chicks fed 400 and 2.000 ppm TDS with betaine supplementation

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