MUHAMMED ENES İNANÇ, KORAY TEKİN, Kemal Tuna OLĞAÇ, DOĞUKAN ÖZEN, CALOGERO STELLETTA, ONGUN UYSAL, ALİ DAŞKIN

Effect of 7-dehydrocholesterol and Cholesterol-loaded Cyclodextrins on Bull Sperm Motility During Short Term Storage [1] [2]

Bu çalışma, kısa süreli saklama süresince (+4°C) 7-dehidrokolesterol doyurulmuş siklodekstrin (7-DHCLC) ve kolesterol ile doyurulmuş siklodekstrinin (CLC) boğa spermasının motilitesinin korunmasındaki etkisinin değerlendirilmesi için yürütülmüştür. Alınan ejekulatlar birleştirilerek 7 gruba ayrıldı, bu gruplardan biri control (C), üçü 7-DHCLC'nin diğer üçü ise CLC'nin farklı konsantrasyonlarını içeren sulandırıcılarla sulandırıldı. Sulandırılan sperma +4°C'ye transfer edildilerek saklandı ve sperma motilitesi üç gün boyunca bilgisayar destekli sperm analiz cihazında (CASA) değerlendirdi. Motilitenin tüm gruplarda azaldığı görüldü fakat, 7-DHCLC ve CLC gruplarında motilite azalmasının daha yavaş olduğu tespit edildi. Fakat, CLC 1.5 mg/120×106grubunun spermanın kısa süreli saklanmasında motiliteyi koruyan en iyi grup olduğu belirlendi. Sonuç olarak, sperma sulandırıcısına değişik oranlarda 7DHCLC ve CLC'nin eklenmesi +4°C'de boğa sperma motilitesini üç gün boyunca koruduğu tespit edildi

7-dehidrokolesterol ve Kolesterol İle Doyurulmuş Siklodekstrinlerin Kısa Süreli Saklama Süresince Boğa Spermasının Motilitesine Etkisi

The study was conducted to determine the effect of 7-dehydrocholesterol loaded cyclodextrin (7-DHCLC) and cholesterol-loaded cyclodextrin (CLC) on changes of bull sperm motility during short term storage (+4°C). Collected ejaculates were pooled and divided into 7 groups, as following one control (C); three 7-DHCLC and the other three CLC concentrations. Diluted semen samples were transferred and stored at +4°C and sperm motility was analysed in determined intervals during three days storage period with computer aided sperm analyse (CASA) system. Motility decreased in all groups; however, 7-DHCLC and CLC groups motility decreased in the next two days gradually. But, CLC 1.5 mg/120x106 group were identified as the best groups for protecting motility in short time preservation. In conclusion, adding different rates of 7-DHCLC and CLC to semen extender preserved motility for three days in bull semen stored at +4°C

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1. Yurdakok B, Tekin K, Daskin A, Filazi A: Effect of polychlorinated biphenyls 28,30 and 118 on bovine spermatozoa in vitro. Reprod Domest Anim, 50, 41-47, 2015. DOI: 10.1111/rda.12447
2. Ari UC, Kulaksiz R, Ozturkler Y, Lehimcioglu NC, Yildiz S: Effect of nacetylcysteine (NAC) on post-thaw semen quality of Tushin rams. Kafkas Univ Vet Fak Derg, 22 (6): 883-887, 2016. DOI: 10.9775/kvfd.2016.15558
3. Buyukleblebici O, Buyukleblebici S, Tuncer PB, Ozgurtas T, Tasdemir U: Effect of α-lipoic acid supplementation to extender on quality of frozen-thawed bull semen. Kafkas Univ Vet Fak Derg, 21 (3): 307- 311, 2015. DOI: 10.9775/kvfd.2014.12366
4. Tasdemir U, Tuncer PB, Buyukleblebici S, Ozgurtas T, Durmaz E, Buyukleblebici O: Effect of various antioxidants on cryopreserved bull sperm quality. Kafkas Univ Vet Fak Derg, 20 (2): 253-258, 2014. DOI: 10.9775/kvfd.2013.9964
5. Purdy PH, Graham JK: Effect of cholesterol-loaded cyclodextrin on the cryosurvival of bull sperm. Theriogeneology, 48: 36-45, 2004. DOI: 10.1016/j.cryobiol.2003.12.001
6. Kmenta I, Strohmayer C, Muller- Schlosser F, Schafer-Somi S: Effects of lecitin and catalase containing semen extender and a second dilution with different enhancing buffers on the quality of cold-stored canine spermatozoa. Theriogenology, 75, 1095-1103, 2011. DOI: 10.1016/j. theriogenology.2010.11.018
7. Althouse GC: Sanitary procedures for the production of extended semen. Reprod Domest Anim, 43, 374-378, 2008. DOI: 10.1111/j.1439- 0531.2008.01187.x
8. Vidal AH, Bastina AM, Bento da Silva EC, Gomes WA, Pelinca MA, Silva SV, Guerra MMP: Soybean lecitin-based extender as an alternative for goat sperm cryopreservation. Small Ruminant Res, 109, 47-51, 2013. DOI: 10.1016/j.smallrumres.2012.07.022
9. Chelucci S, Pasciu V, Succu S, Addis D, Leoni GG, Munca ME, Naitana S, Berlinguer F: Soybean lecithin-based extender preserves spermatozoa membrane integrity and fertilizing potential during goat semen cryopreservation. Theriogenology, 83, 1064-1074, 2015. DOI: 10.1016/j.theriogenology.2014.12.012
10. Amorim EAM, Graham JK, Spizziri B, Meyers M, Torres CAA: Effect of cholesterol or cholesteryl conjugates on the cryosurvival of bull sperm. Cryobiology, 58, 210-214, 2009. DOI: 10.1016/j.cryobiol.2008.12.007
11. Stoll C, Stadnick H, Kollas O, Holovati JL, Glasmacher B, Acker JB, Wolkers WF: Liposomes alter thermal phase behaviour and composition of red blood cell membranes. Biochimica et Biophysica Acta (BBA)- Biomembranes, 1808, 474-481, 2011. DOI: 10.1016/j.bbamem.2010.09.012
12. Vyas S, Mohan G, Dham AJ, Sahn KL: Studies on the norms and correlations of initial and post thaw seminal attributes of triple crossbred bull. Int J Anim Sci, 7, 73 -76, 1992.
13. Franceschini NPH, Nhe RP, Lel LTFG, Olne Ra FLEB, Esper CR: Effects of storage on sperm motility in refrigerated boar semen. Brasilian Reprod Anim, 8, 91-95, 1984.
14. Medeiros CMO, Forell F, Oliveria ATD, Rodrigues JL: Current status of sperm cryopreservation: why isn’t it better? Theriogenology, 57, 327- 344, 2002. DOI: 10.1016/S0093-691X(01)00674-4