Kittisak BUDDHACHAT, Wannapimol KRIANGWANICH, Isaraporn KUMOUN, Janine L. BROWN, Sasisophin CHAILANGKARN, Chaleamchat SOMGIRD, Chatchote THITARAM, Sukon PRASITWATTANASEREE, Korakot NGANVONGPANIT

Telomeric Attrition with Increasing Age in Short- (Chihuahua Dog) and Long- (Asian Elephant) Life Span Animals

Bu çalışmada, kısa (Chihuahua köpek) ve uzun (Asya fili) ömürlü hayvanlarda artan yaş ile birlikte telomerik yıpranma oranı gerçek zamanlı kantitatif PCR (qPCR) kullanılarak araştırıldı. Yaşları 24-840 hafta arasında değişen toplam 122 Asya fili (106 dişi ve 16 erkek) ile 1-179 aylık Chihuahua (65 dişi ve 24 erkek) çalışmada kullanıldı. Genç Asya filleri (pre- ve peri-puberte) köpekler ile karşılaştırıldığında daha fazla orantısal telomer uzunluğuna sahip olduğu tespit edildi. Hem Chihuahua (R2=0.0490, P=0.0017) hem de Asya fillerinde (R2=0.0177, P=0.0210) orantısal telomer uzunluğu ile artan yaş arasında düşük ama anlamlı negatif yönlü bir ilişki gözlemlendi. Her iki tür için erkek ve dişi hayvanlardaki tahmini telomer kayıp oranı 0.0023 ile 0.0065 arasında değişirken tür veya cinsiyet yönünden bir fark tespit edilmedi. Elde edilen sonuçlar daha fazla orantısal telomer uzunluğuna sahip olan Asya fillerinin Chihuahua köpeklerden daha uzun telomer ile başladıklarını ancak daha sonra telomer yıpranmanın her iki türde de benzer oranda şekillendiğini gösterdi. Hem Chihuahua köpeklerde hem de Asya fillerinde orantısal telomer uzunluğundaki varyasyonun sadece küçük bir yüzdesi yaşa bağlıdır. Bu sebeple, yaş tayininde bir biyolojik araç olarak kullanılması bu türlerde sınırlı gözükmektedir

Kısa (Chihuahua köpek) ve Uzun (Asya fili) Ömürlü Hayvanlarda Artan Yaş İle Birlikte Telomerik Yıpranma

Here, we explored the rate of telomere attrition with increasing age by real-time quantitative PCR (qPCR) in a short- (Chihuahua dog) and long(Asian elephant) lived species. A total of 122 Asian elephants (female = 106, male = 16) ranging from 24-840 months of age, and 89 Chihuahuas (female = 65, male = 24) 1-179 months of age were used in this study. We found that young (pre- and peri-pubertal) Asian elephants had a higher relative telomere length (RTL) compared to dogs. A low, but significant negative relationship between RTL and increasing age was observed in both Chihuahuas (R2=0.0490, P=0.0017) and Asian elephants (R2=0.0177, P=0.0210). The estimated rate of telomere loss for males and females of both species ranged from -0.0023 to -0.0065, with no clear differences between gender or species. Results suggest that Asian elephants may start with longer telomeres than Chihuahuas, as RTL was higher, but then the rate of telomere attrition proceeds at a similar rate in both species. Age accounted for only a small percentage of the variation in RTL in both Chihuahua dogs and Asian elephants, however. Thus, its use as a biological tool for age estimation would appear to be limited for these species

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