Rini Devijanti RIDWAN, Tantiana TANTIANA, Darmawan SETIJANTO, Astika Kinant KUSUMA, Ananda Firman PUTRANTO

Kronik Periodontitli Wistar Sıçan (Rattus Novergicus)’larda Elektrolize İndirgenmiş Suyun Antioksidan ve Antiinflamatuvar Etkisi

Kronik periodontitis, ROS gibi çeşitli metabolitleri salan ve böylece lipit peroksidasyonun biyolojik bir biyobelirteci olan malondialdehit (MDA)’in üremesine neden olan makrofajların aktivasyonuyla karakterize bir yangıdır. Bu çalışmanın amacı; kronik periodontitli Wistar sıçanlarda elektrolize indirgenmiş su (EİS)’yun malondialdehit seviyesi ile makrofaj ve lenfosit sayılarına etkilerini araştırmaktır. Çalışma deneysel olup rastgele örnekleme metodu kullanılmıştır. Yirmi bir Wistar sıçanın mandibular molar dişlerinin proksimal bölgelerine 106 Porphyromonas gingivalis uygulandı ve günlük ağız yoluyla belirtilen uygulamaları gerçekleştirmek için üç gruba ayrıldı; Kontrol negatif grubu (distile su), Kontrol pozitif grubu (1.08 mg/200 g vücut ağırlığı/gün Vitamin C) ve Uygulama grubu (20 mL EİS). Gruplar 1. günden 14. güne kadar gözlemlendi. Deneklerden gingival doku örnekleri 14. günde alınarak malondialdehit analizi ile makrofaj ve lenfosit sayımları yapıldı. Verilerin analizi Saphiro-Wilk normalite testi ve Varyansların eşitliği için Levene Testi veya Kruskal Wallis testine (P>0.05) dayanarak Tek yönlü varyans analizi (ANOVA) ve Mann Whitney testi (P

The Ability of Electrolyzed Reduced Water to Act as an Antioxidant and Anti-Inflammatory Agent in Chronic Periodontitis Wistar Rats (Rattus novergicus)

The chronic periodontitis was inflammation characterized by macrophage activation which releases various metabolites such as ROS that, in turn, produces malondialdehida (MDA) a biological biomarker of lipid peroxidation. The aim of this research is to analyze the effect of Electrolyzed Reduced Water (ERW) on the levels of malondialdehyde, macrophage and lymphocytes cells in Wistar rats suffering from chronic periodontitis. It constitutes an experimental laboratory study incorporating a random sampling method. Twenty-one Wistar rats were induced with up to 106 Porphyromonas gingivalis in the proximal area of the mandibular molar and divided into three groups which were administered orally on a daily basis as follows: a control negative group (distilled water); a control positive group (a dose of vitamin C at 1.08 mg/200 g Body Weight/day) and a treatment group (20 mL ERW) and observed between Day 1 and Day 14. Samples of gingival tissue were taken from the subjects for analysis with malondialdehyde and the conducting of macrophage and lymphocyte cell counts on Day 14. Data analysis comprised a One-Way Analysis of Variance (ANOVA) and a Mann Whitney test (P0.05). ERW can decrease the level of malondialdehyde (4.3±0.7), the number of macrophage cells (19.4±8.6) and the number of lymphocyte cells (8.5±0.8) in the treatment group. The ERW mechanism can, therefore, be said to suppress the occurrence of further tissue damage triggered reactive oxygen species (ROS) in Chronic Periodontitis.

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1. Hamrun M, Nurlindah H, Hatta M: Vitamin D gene receptor polymorphism on chronic periodontitis patient. MSc Thesis, Department of Oral Biology, Faculty of Dentistry, Hasanuddin University, Makassar, 165172, 2011.
2. Mane AK, Karmakar AP, Bharadwaj RS: Anaerobic bacteria in subjects with chronic periodontitis and in periodontal health. J Oral Health Comm Dent, 3 (3): 49-51, 2009.
3. Susanti A, Yuniastuti Y, Iswari R: Reactive oxygen species activity and makrofag after aloe vera gel stimulation in Salmonella typhimurium infection. J Math Nat Sci, 35 (1): 2-20, 2012.
4. Purwantaka P: Validation of deoxyribose methods as a radical test of arrest hydroxyl free by vitamin C in-vitro. MSc Thesis, Fakultas Farmasi Universitas Sanata Dharma Yogyakarta, 23-30, 2005.
5. Chambron J: Physico-chemical, biological and therapeutic characteristics of electrolyzed reduced alkaline water (ERAW). Water, 5, 2094-2115, 2013. DOI: 10.3390/w5042094
6. Arifah RA: The effect of alkaline drinking water to increase the number of plasma cells in chronic inflammation. Undergradutate Thesis, Fakultas Kedokteran Gigi. Universitas Airlangga Surabaya, 35-47, 2015.
7. Freeman KJ: The alkaline lifestyle approach to healing diabetes. In, Carlsbad, Alkaline People Publishing. 1st ed., 37-40, Hawking Book, Corona, California, United, 2014.
8. Shirahata S: Advanced research on the healt benefit of reduce water. MSc Thesis, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Japan, 125-127, 2012.
9. Kasuyama K, Tomofuji T, Ekuni D, Tamaki N, Azuma T, Irie K, Endo Y, Morita M: Hydrogen-rich water attenuates experimental periodontitis in rat model. J Clin Periodontol, 38 (12): 1085-1090, 2011. DOI: 10.1111/j.1600051X.2011.01801.x
10. Choi W, Hee-Souk K, Hyeon YL: Enhancement of anti-skin inflammatory activities of scutellaria baicalensis using an alkaline reduced water extraction process. MSc Thesis, Department of Medical Biomaterials Engineering, Kangwon National University, Chuncheon, Gangwon, Korea, 200-701, 2014.
11. Lee MY, Kim YK, Ryoo KK, Lee YB, Park EJ: Electrolyzed-reduced water protects agains oxidative damage to DNA, RNA, and protein. Appl Biochem Biotechnol, 135 (2): 133-144, 2006.
12. Ohsawa I, Ishikawa M, Takahashi K, Watanabe M, Nishimaki K, Yamagata K, Katsura K, Katayama Y, Asoh S, Ohta S: Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals. Nat Med, 13, 688-694, 2007. DOI: 10.1038/nm1577
13. Dovi JV, Szpaderska AM, DiPietro LA: Neutrofil function in the healing wound: adding insult to injury, burn and shock trauma institute. Illinois, USA. MSc Thesis, Departement of Surgery, Loyoda University Medical Center Maywood, 275-280, 2006.
14. Staudte H, Sigusch BW, Glockmann E: Grapefruit consumption improves vitamin C status in periodontitis patients. Br Dent J, 199, 213217, 2005. DOI: 10.1038/sj.bdj.4812613
15. Muhammad I: Vitamin C antioxidant effects on male wistar rats (Rattus norvegicus L) affected by smoking smoke. Postgraduate School. MSc Thesis, Institute Pertanian Bogor, 20-25, 2009.
16. Aghazy AZZ: The antioxidant effect of green tea extract (Camellia Sinensis L.) chronic periodontitis patient’s saliva. Undergradutate Thesis, Fakultas Kedokteran Gigi. Universitas Airlangga Surabaya Thesis, 50-65, 2015.