Selim ALCAY, Selvinar SEVEN ÇAKMAK, İbrahim ÇAKMAK, Emine MÜLKPINAR, Mehmed Berk TOKER, Burcu ÜSTÜNER, Hasan ŞEN, ZEKARİYA NUR

Drone Semen Cryopreservation with Protein Supplemented TL-Hepes Based Extender

The aim of the current study was to determine the optimum concentration of bovine serum albumin for post-thawing quality of drone sperm and this is the first study to evaluate the effect of BSA supplemented TL-Hepes based extenders for drone semen cryopreservation. Sexually mature drones were used for semen collection. Pooled semen was diluted with TL-Hepes based extender supplemented with different concentrations of BSA (1 mg/mL, 3 mg/mL, and 5 mg/mL) or without BSA (control), at a final concentration of 100x106 spermatozoon/mL. Motility, plasma membrane functional integrity (HOST), and defected acrosome (PSA-FITC) were evaluated in the study. At post thaw, the highest sperm motility rates were obtained in the BSA5 group (P

Arı Spermasının Protein Eklenmiş TL-Hepes Bazlı Sulandırıcı İle Dondurulması

Bu çalışmanın amacı, arı spermasının çözdürme sonrası kalitesi için en uygun sığır serum albümin konsantrasyonunu belirlemekti ve bu çalışma, arı spermasının dondurulması için BSA eklenmiş TL-Hepes bazlı sulandırıcıların etkisini değerlendiren ilk çalışmadır. Cinsel olarak olgun arılar sperma toplanması için kullanıldı. Alınan spermalar bir araya getirildikten sonra final konsantrasyonu 100x106 spermatozoon/ mL olacak şekilde farklı dozlarda BSA içeren (1 mg/mL, 3 mg/mL, ve 5 mg/mL) ve içermeyen (kontrol) TL-Hepes bazlı sulandırıcılarla sulandırılmıştır. Spermanın değerlendirilmesi amacıyla motilite, plazma membran fonksiyonel bütünlüğü (HOST), ve akrozomal bozukluk (PSA-FITC) değerlerine bakılmıştır. Eritme sonrası en yüksek motilite oranları BSA5 grubunda elde edilmiştir (P

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