Selim ALCAY, Selvinar CAKMAK, Ibrahim CAKMAK, Ahmet AKTAR, Melih YILMAZ, Burcu USTUNER, Mustafa AKKASOGLU, Seyma TASKIRAN, Elif AYAZ, Hakan SAĞIRKAYA, ZEKARİYA NUR

L-Carnitine Supplemented Extenders Improve Post-Thawing Quality of Honey Bee Drone (Apis mellifera) Spermatozoa

The study aimed to evaluate the eff ects of L-carnitine on honey bee (Apis mellifera) drone spermatozoon at post-thaw. Semen samples obtained from sexually mature drones were pooled. Then, pooled semen was diluted with diff erent concentrations of L-carnitine (2.5 mM/ 5 mM/ 10 mM) and without L-carnitine (control) supplemented extenders. Motility, plasma membrane functional integrity, acrosomal integrity and mitochondrial function were negatively aff ected by the cryopreservation process but DNA integrity was not aff ected. Malondialdehyde (MDA) concentration was used for analyzing the membrane lipid peroxidation status and a better result was obtained in the LC5 group compared with the control group at post-thaw. L-carnitine groups had a positive eff ect on sperm motility, plasma membrane integrity, and mitochondrial function compared to the control group (P

L-Karnitin İlave Edilmiş Sulandırıcılar Bal Arısı (Apis mellifera) Spermatozoası’nın Çözdürme Sonrası Kalitesini Arttırır

Bu çalışmada, L-karnitinin eritme sonrası bal arısı (Apis mellifera) spermatozoonu üzerindeki etkilerini değerlendirme amaçlandı. Cinsel olarak olgun erkek arılardan elde edilen sperm örnekleri birleştirildi. Daha sonra birleştirilen semen, farklı konsantrasyonlarda L-karnitin içeren (2.5 mM/5 mM/10 mM) ve içermeyen (kontrol) sulandırıcılar ile seyreltildi. Motilite, plazma membranı fonksiyonel bütünlüğü, akrozomal bütünlük ve mitokondriyal fonksiyon kriyoprezervasyon sürecinden olumsuz etkilenmiş, ancak DNA bütünlüğü etkilenmemiştir. Malondialdehit (MDA) konsantrasyonu, membran lipid peroksidasyon durumunu analiz etmek için kullanıldı ve çözdürme sonrası kontrol grubuna kıyasla LC5 grubunda daha iyi bir sonuç elde edildi. L-karnitin grupları, kontrol grubuna kıyasla sperm motilitesi, plazma membran bütünlüğü ve mitokondriyal fonksiyon üzerinde olumlu bir etkiye sahipti (P

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