Kars yöresinde atık yapmış inek sürülerinden alınan süt ve vajinal sıvap örneklerinden Brusella etkenlerinin bakteriyolojikve moleküler tanımlanması

Kars merkez ve Selim ilçesine bağlı köylerde atık yaptığı bilinen sığır sürülerinde bulunan ve hastalığa karşı aşılanmamış 250 inekten elde edilen süt ve vajinal sıvap örnekleri olmak üzere toplam 500 örnek Brusella cinsi bakteriler yönünden kültürelolarak değerlendirildi. Bakteriyolojik inceleme sonucunda 250 süt örneğinin 11 (%4.4)’inden ve 250 vajinal sıvap örneğinin 16 (%6.4)’sından olmak üzere toplam 27 (%5.4) örnekten Brucella spp. izolasyonu yapıldı. Bu izolatların, Brusella cinsine (Ompbp26) ve eritritol (ery) genine spesifik primerle yapılan PCR incelemesinde hepsinin Brucella spp. ve saha suşları olduğu görüldü.İzole edilen tüm suşlar B. abortus biyotip 3 olarak tiplendirildi. Ayrıca bu izolatların RAPD-PCR ile incelemesinde elde edilen DNA profillerinin, B. abortus S19 ile homoloji gösterdiği tespit edildi.

Anahtar Kelimeler:

vajina, Brucella abortus, Türkiye, yavru atma, bakteriyel hastalıklar, biyotip, sığır, inek, süt sığırı, süt ineği, süt, soy, Kars

Bacteriological and molecular description of Brucella species isolated from milk and vaginal swab samples of aborted cattle in Kars Region

In this study, the milk and vaginal swab samples, collected from each of 250 cows raised in Kars and Selim areas, were analysed for culturing Brucella species. These animals had a history of abortion and they were unvaccinated against Brucellosis. Based on the bacteriological examination, Brucella spp. was isolated from total of 27 (%5.4) samples, 11 (%4.4) and 16 (%6.4) originated from milk and vaginal swab samples, respectively. These isolates were found to be Brucella spp. Are field isolates based on the PCR analysis using primers specific to Brucella genus (Omp bp26) and erythritol (ery). All of the isolated strains were typed as B. abortus biotype 3. RAPD-PCR analysis showed that DNA patterns of these isolates were found to be similar to that of B. abortus S19.

Keywords:

vagina, Brucella abortus, Turkey, abortion, bacterial diseases, biotypes, cattle, cows, dairy cattle, dairy cows, milk, strains, Kars,

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