ALEV GÜROL BAYRAKTAROĞLU, DENİZ KORKMAZ, Resat Nuri ASTI, NEVİN KURTDEDE, HİKMET ALTUNAY

Conjunctiva associated lymphoid tissue in the ostrich (Struthio camelus)

Bu çalışma devekuşunda (Struthio camelus) konjunktiva ilişkili lenfoid doku (CALT)’nun yapısını ortaya koymak amacıyla yapıldı. Mezbahaneden sağlanan 5 adet sağlıklı erişkin devekuşu alt ve üst göz kapakları çalışma materyali olarak alındı. Agregat lenf foliküllerinin alt palpebral konjunktivada fibröz flap’ın altında geniş bir alana yayıldığı, üst palpebral konjunktivada ise dağılımlarının nazolakrimal punktuma yakın bölgelerde sınırlı olduğu görüldü. Işık mikroskobik incelemede konjunktiva epiteli, non-lenfoid ve lenfoid bölgeler arasında farklılık gösteriyordu. Non-lenfoid bölgeler kadeh hücresi içeren yalancı çok katlı prizmatik epitele sahipti. Lenfoid bölgelerde ise folikül ilişkili epitel (FAE) kadeh hücresi içermeyen ve tek katlı yassı epitelden oluşmaktaydı. Bu bölgelerde subepitelyumda agregat ve/veya soliter lenf folikülleri bulunmaktaydı. Lenf foliküllerinde dome, germinal merkez, korona ve interfoliküler bölgeleri ayırt ediliyordu. Ayrıca interfoliküler alanlarda lenfosit göçünde görev alan yüksek endotelli venüller (HEV) görüldü. Elektron mikroskobik incelemelerde, FAE’nin immun sistem hücreleri ile yakın ilişkide olduğu gözlendi. FAE’de tipik membranöz hücrelere (M hücreleri) benzer şekilde kısa, kalın ve az sayıda mikrofoldlar dikkat çekti. CALT’ın makroskobik, mikroskobik ve ultrastruktural yapısı üzerinde elde edilen bulgular ile; bu dokunun devekuşundaki (Struthio camelus) yapısı ortaya konuldu.

Anahtar Kelimeler:

gözler, devekuşu, lenfositler, epitel, konjunktiva, hayvan anatomisi

Devekuşunda (Struthio camelus) konjunktiva ilişkili lenfoid doku

This study was aimed at the investigation of the structure of conjunctiva-associated lymphoid tissue (CALT) in the ostrich(Struthio camelus). The upper and lower eyelids of 5 healthy adult ostriches obtained from a slaughterhouse constituted thematerial of the study. Aggregated lymphoid follicles occupied a large area beneath the fibrous flap in the lower palpebralconjunctiva, while their distribution in the upper palpebral conjunctiva was observed to be limited to regions near thenasolacrimal punctum. Light microscopic examination revealed the conjunctival epithelium to differ between non-lymphoidand lymphoid regions. Non-lymphoid regions had pseudostratified prismatic epithelium containing goblet cells. Follicle-associated epithelium (FAE) in lymphoid regions was composed of single-layered squamous epithelium without goblet cells.Aggregated and/or solitary lymphoid follicles were present in the subepithelium. Dome area, germinal centre, corona andinterfollicular region were able to be distinguished in lymphoid follicles. Furthermore, interfollicular regions contained highendothelial venules, which are involved in lymphocyte migration. Electron microscopic examination demonstrated that FAE wasclosely associated with immune system cells. Cells, which resembled typical M cells in having short, thick apical micro-folds and vesicles, were present in the FAE. In the present study, the structure of CALT was demonstrated in the ostrich (Struthio camelus), based on macroscopic, microscopic and ultrastructural findings.

Keywords:

eyes, ostriches, lymphocytes, epithelium, conjunctiva, animal anatomy,

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