Effect of different doses PMSG on Estrus synchronization and fertility in awassi ewes synchronized with progesterone duringthe transition period
Sunulan çalışmada, geçiş döneminde progesteron ile senkronize edilen İvesi koyunlarda östrus senkronizasyonu ve fertilite üzerine PMSG’nin farklı dozlarının etkisinin değerlendirilmesi amaçlandı. Bu çalışmada toplam 92 başİvesi koyun kullanıldı. Çalışmada yer alan koyunlara 30 mg fluorogestone acetate (FGA) içeren süngerler 12 gün süreyle intravaginal olarak yerleştirildi. Koyunlar rastgele 4 gruba ayrıldı ve süngerler çıkarıldıktan sonra grup 1’e (n=21) 300 IU; grup 2’ye (n=27) 400 IU; grup 3’e (n=25) 500 IU PMSG, grup 4’e (n=19) ise 1 ml serum fizyolojik intramuskuler yolla enjekte edilerek kontrol grubu olarak bırakıldı. Süngerler geri alındıktan sonra östruslar sırasıyla; grup 1, 2, 3 ve kontrol grubunda 40.82±1.21, 40.20±1.14, 38.7±1.07 ve 41.79±1.72 saatlerde görüldü. Östruslar; grup 1 de, %81.0; grup 2 de, %92.6; grup 3 de, %92.0 ve kontrol grubunda ise %73.7 olarak tüm gruplarda benzer oranlarda gözlemlendi. Östrus oranları ve östrusların görülme zamanı açısından PMSG grupları ile kontrol grubu arasında istatistiksel açıdan bir farkın olmadığı belirlendi. Östrus süresinin, kontrol grubu ile karşılaştırıldığında grup 1’de daha kısa sürdüğü (24.9±0.85 ve 29.07±1.31 saat) ve istatistiksel açıdan önemli olduğu belirlendi (P
Geçiş döneminde progesteron ile senkronize edilen ivesi koyunlarda PMSG’nin farklı dozlarının östrus senkronizasyonuve fertilite üzerine etkisi
The aim of the present study was to evaluate different of doses PMSG on estrus synchronization and fertility in Awassi ewessynchronized with progesterone during The transition period. A total of 92 ewes were used in this experiment. All ewes were treated witha vaginal sponge containing 30 mg fluorogestone acetate (FGA) inserted into the vagina of the ewes for 12 days. All animals were dividedinto four groups randomly and a single intramuscular (IM) dose of PMSG (group 1, 300 IU, n=21; group 2, 400 IU, n=27; group 3, 500 IU,n= 25), group 4 (n=19) was injected with 1 ml normal saline solution and as served control group at time of sponge removal. The intervalbetween the withdrawal of the sponges and estrus was observed 40.82±1.21 h, 40.20±1.14 h, 38.7±1.07 h and 41.79±1.72 h in groups 1, 2, 3 and the control group, respectively. Estrus responses were similar in all groups (group 1, 81.0%; group 2, 92.6%; group 3, 92.0%;control group, 73.78%). There were no statistically significant differences (P>0.05) between the treatment groups and the control groupfor the onset of estrus or estrus response. The duration of estrus was shorter in group 1, compared to the control group (24.9±0.85 hrvs. 29.07±1.31hr), and that this difference was statistically significant (P<0.05). Pregnancy rates were 82.35%, 80.0%, 82.60% and 78.5% ingroups 1, 2, 3 and the control group, respectively. Lambing rates were obtained 100.0% among all the groups, or litter size (1.07 to 1.21)did not differ between the treatment groups and the control group. As a result, different doses of PMSG in Awassi ewes synchronized withprogesterone during the transition period had similar effect on estrus synchronization and fertility parameters.
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- 1. Lymberopoulos AG, Boscos CM, Dellis S, Papia A, Belibasaki S: Oestrous synchronization under range conditions in dairy goats treated with diff erent PGF2α doses during the transitional period in Greece. Anim Sci, 75, 289-294, 2002.
- 2.Ungerfeld R, Rubianes E: Short term primings with diff erent progestogen intravaginal devices (MAP, FGA and CIDR) for eCGestrous induction in anestrus ewes. Small Rumin Res, 46, 63-66, 2002.
- 3.Husein MQ, Kridli RT: Effect of progesterone prior to GnRH-PGF2α treatment on induction of oestrus and pregnancy in anoestrous Awassi ewes. Reprod Dom Anim, 38, 228-232, 2003.
- 4.Lindsay DR: Reproduction in the sheep and goat. In, Cupps PT (Ed): Reproduction in Domestic Animals. pp. 491-515, Academic Press, Inc., California, 1991.
- 5.Wildeus S: Current concepts in synchronization of estrus: Sheep and goats. Pro Am Soc Anim Sci, 1-14, 1999.
- 6.Epstein H: Biology of reproduction, suckling regimes, growth, and development. In, The Awassi sheep with special reference to the improved dairy type. Rome: FAO. pp. 81-140, 1985.
- 7.Mason IL: World Dictionary of Livestock Breeds. 3th ed. CAB International. pp. 348, 1988.
- 8.Zarkawi M, Al-Merestani MR, Wardeh MF: Induction of synchronized oestrus and early pregnancy diagnosis in Syrian Awassi ewes, outside the breeding season. Small Rumin Res, 33, 99-102, 1999.
- 9. Al-Merestani MR, Zarkawi M, Wardeh M: Early breeding and pregnancy diagnosis in Syrian Awassi sheep yearlings. Reprod Dom Anim, 34, 413-416, 1999.
- 10. Kridli RT, Husein MQ, Muhdi HA, Al-Khazaleh JM: Reproductive performance of hormonally-treated anestrous Awassi ewes. Anim Reprod, 3, 347-352, 2006.
- 11. Whitley NC, Jackson DJ: An update on estrus synchronizati on in goats: a minor species. J Anim Sci, 82 (Suppl. E), E270-E276, 2004.
- 12. Jainudeen MR, Wahid H, Hafez ESE: Ovulation induction, embryo production and transfer. In’ Reproduction in farm animals.’ EDS : B Hafez/ESE Hafez, 7th ed., pp. 405-430, Lippincott Williams-Wilkins, Baltimore, USA, 2000.
- 13. Boscos CM, Samartzi FC, Dellid S, Rogge A, Stefanakis A, Kramboviti s E: Use of progestagen-gonadotrophin treatment in estrus synchronization of sheep. Theriogenology, 58, 1261-1272, 2002.
- 14. Timurkan H, Yildiz H: Synchronization of oestrus in Hamdani ewes: The use of different PMSG doses. Bull Vet Inst Pulawy, 49, 311-314, 2005.
- 15. Zeleke M, Greyling JPC, Schwallbach LMJ, Muller T, Erasmus JA: Effect of progestagen and PMSG on oestrous synchronization and fertility in Dorper ewes during the transiti on period. Small Rumin Res, 56, 47-53, 2005.
- 16. Spss For Window. Release10.0.1, 1999.
- 17. Godfrey RW, Collins JR, Hensley EL, Wheaton JE: Estrus synchronization and artifi cial insemination of hair sheep ewes in the tropics. Theriogenology, 51, 985-997, 1999.
- 18. Iida K, Kobayashi N, Kohno H, Miyamoto A, Fukui Y: A comparative study of induction of estrus and ovulation by tree different intravaginal devices in ewes during the non-breeding season. J Reprod Dev, 50, 63-69, 2004.
- 19. Kohno H, Okamoto C, Iida K, Takeda T, Kaneko E, Kawashima C, Miyamoto A, Fukui Y: Comparison of estrus induction and subsequent fertility with two different intravaginal devices in ewes during the non-breeding season. J Reprod Dev, 51, 805-812, 2005.
- 20. Karagiannidis A, Varsakeli S, Karatzas G, Brozos C: Eff ect of time of artifi cial insemination on fertility of progestagen and PMSG treated indigenous Greek ewes, during non-breeding season. Small Rumin Res, 39, 67-71, 2001.
- 21. Luther JS, Grazul-Bilska AT, Kirsch JD, Weigl RM, Kraft KC, Navanukraw C, Pant D, Reynolds LP, Redmer DA: The eff ect of GnRH, eCG and progestin type on estrous synchronizati on following laparoscopic AI in ewes. Small Rumin Res, 72, 227-231, 2007.
- 22. Ataman MB, Aköz M, Akman O: Induction of synchronized oestrus in Akkaraman cross-bred ewes during breeding and anestrus season: The use of short-term and long-term progesterone treatments, Revue Méd Vét, 157, 257-260, 2006.
- 23. Ataman MB, Aköz M, Fındık M, Saban E: Geçiş dönemi başındaki Akkaraman melezi koyunlarda farklı dozda fl ourogestene acetate, norgestomet ve PGF2α ile senkronize östrüslerin uyarılması. Kafkas Univ Vet Fak Derg, 15, 801-805, 2009.
- 24. Aköz M, Bülbül B, Ataman MB, Dere S: Induction of multiple births in Akkaraman cross-bred sheep synchronized with short duration and different doses progesterone treatment combined with PMSG outside the breeding season. Bull Vet Inst Pulawy, 50, 97-100, 2006.
- 25. Dogan I, Nur Z: Different estrous induction methods during the non-breeding season in Kivircik ewes. Vet Med, 51, 133-138, 2006.
- 26. Kridli RT, Al-Kheti p SS: Reproductive responses in ewes treated with eCG or increasing doses of royal jelly. Anim Reprod Sci, 92, 75-85, 2006.
- 27. Ustuner B, Gunay U, Nur Z, Ustuner H: Effect of long and short- term progestagen treatments combined with PMSG on oestrus synchronization and fertility in Awassi ewes during the breeding season. Acta Vet Brno, 76, 391-397, 2007.
- 28. Koyuncu M, Uzun SK, Sengül L: Kıvırcık koyunlarında progesteron ve farklı dozda PMSG kullanımının kızgınlık denetimi ve döl verimini arttı rma olanakları. Turkish J Anim Sci, 25, 971-974, 2001.