Karbon tetraklorür ($CCl_4$) ve etil alkol'ün fare eritrosit antioksidan ve plazma lipid peroksidasyonuna etkisi

Bu çalışmada karbon tetraklorür ve etil alkol ile karaciğer dejenerasyonu oluşturulan farelerde lipid peroksidasyonunun göstergesi olan malohdialdehid (MDA) ve antioksidan savunma sistemindeki değişiklikler incelenerek dejenerasyon ile ilişkileri araştırıldı. Çalışmada 30 adet Swiss albino erkek fare (25-30 gr) deneme öncesi bir hafta boyunca adaptasyon, sağlamaya yönelik olarak standart fare yem ve su ile ad libitum olarak beslendi. Daha sonra tesadüfi olarak 3 eşit gruba ayrılan hayvanlardan I. Gruba (kontrol grubu ) standart fare yemi ve su, II. gruba standart fare yemi ve 2 ml/kg $CCl_4$, III. gruba ise standart fare yemi + % 50'lik etil alkolden 3 ml/kg cdanlı ağırlık odozda oral olarak 10 hafta boyunca her gün verildi. Kontrol grubu ile deneme grupları arasında yaptığımız kıyaslamada MDA düzeylerinde önemli oranda (p

Anahtar Kelimeler:

antioksidanlar, karbon tetraklorür, enzim aktivitesi, etanol, yem bileşimi tablosu, karaciğer, malonaldehit, fareler, toksisite

Effect of carbon tetrachloride and ethil alchol in plasma lipid peroxidation and erythrocyte antioxidant of the mice

Changes in the levels of malondiaidehide (MDA, a degradative product of lipid peroxidation) and in the antioxidant defens system in rats with carbontetrachloride and ethanol-induced liver degeneration were studied, in order to determine whethere is any reation between these parameters and disease. Three week old clinically healthy famale Swiss Albino mice (n= 30) weighing 25-30 g were used in this study. The animals were allowed to acclimatize for 15 days and randomly assorted into following groups: Group I (n=10) Animals were put on a normal diet and sham-treated with 2 ml/kg distilled water through oral gavage, three times a week for 10 weeks this group of animals served as control. Group II (n= 10) Animals were put on a normal diet and treated with 2 ml/kg B. W $CCl_4$ dissolved in 2 ml distilled water through oral gavage, three times a week for 10 weeks. Group III (n=10) Animals were put on a normal diet and treated with 3 ml/kg b w ethanol through oral evryday for 10 weeks. In the present study, plasma MDA levels of the $CCl_4$ and ethanol group were markedly higher than controls (P<0.01.) On the other hand, erythrocyte GSH and GSH-Px levels weie significantly low when compared with controls. The difference was statistically significant for catalase activity (P<0 005)

Keywords:

antioxidants, carbon tetrachloride, enzyme activity, ethanol, feed composition tables, liver, malonaldehyde, mice, toxicity,

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