Localisations of the neurophysin and $beta$-endorphin with orexin-A labelling in the rat spinal cor

Yeni bir nöropeptit olan hipokretin (öreksin) son yıllarda lateral hipotalamusun sinir hücrelerinde ortaya çıkarılmış olup, besin maddesi alımının artmasında ve endokrin sistemin düzenlenmesinde rolü olduğu düşünülmektedir. Bu çalışmada, on adet Wistar ratin omuriliğinde, oreksin-A ile nörofizin ve ß-endorfin içeren sinir hücre ve fibrillerinin lokalizasyonunu ortaya çıkarmak amacıyla İmmunohistokimyasal yöntem kullanıldı. Omuriliğin tüm dorsal kutnularının yüzeyinde oreksin-A pozitif hücreleri ile birçok tarafa yönelmiş fibril ve terminasyonları görüldü. Nörofizin ve ß-endorfin hücreleri ile bunlara ait fibril ve terminasyonları da aynı bölgede bulundu. Bu çalışma öreksin içeren sinir hücrelerinin birbirleriyle alakalı birçok sistemdeki fonksiyonların düzenlenmesinde önemli bir role sahip olabileceğini ortaya çıkarmıştır. Ayrıca, omuriliğin yüzeysel kısımlarında oreksinin muhtemelen ağrı modülasyonu, beslenme ve nöroendokrin fonksiyonların düzenlenmesinde rolü olabileceğini düşündürmüştür.

Anahtar Kelimeler:

biyokimyasal teknikler, endokrinoloji, endorfin, hipotalamus, immünohistokimya, sinir sistemi, nöropeptitler, nörofizin, beslenme, ağrı (tıp), sıçan, omurilik

Ratların omuriliğinde oreksin-A ile nörofizin ve $beta$-endorfinin immunohistokimyasal yöntem ile lokalizasyonu

The novel neuropeptides called hypocretins (orexins) have recently been described in neurones of the lateral hypothalamus and shown to increase feeding and regulate the endocrine system. In this study, immunohistochemical method was used to map the localization of neurones and fibres containing-orexin-A with -neurophysin and -ß-endorphin in the spinal cord of the teri adult Wistar rats. Orexin-A positive cells with their fibres and terminals that oriented towards varies directions were seen throughout the superficial lamina of the spinal cord. Neurophysin and ß-endorphin cells and their fibres and terminals were observed in the superficial dorsal horn of the spinal cord. The present study indicates thatwexin-containing neurones may have an important role in modulating of a number of interrelated system functions. Additionally, orexin possibly regulates the neuroendocrine functions, feeding and pain modulation in the superficial lamina of the spinal cord

Keywords:

biochemical techniques, endocrinology, endorphins, hypothalamus, immunohistochemistry, nervous system, neuropeptides, neurophysins, nutrition, pain, rats, spinal cord,

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