HİDAYET METİN ERDOĞAN, Ahmet ÜNVER, VEHBİ GÜNEŞ, MEHMET ÇİTİL

Frequency of rotavirus and coronavirus in neonatal calves in Kars district

Bu çalışmada Kars yöresinde neonatal buzağılarda rotavirus ve coronavirusun yaygınlığının belirlemesi amaçlandı. Bölge ve çiftliklerin seçiminde iki aşamalı kotalı tesadüfi örnekleme metodu kullanıldı, çalışma kapsamında çiftliklere haftalık ziyaretler yapılarak neonatal buzağıların klinik muayeneleri gerçekleştirildi. Latex aglitünasyon ve ELISA kullanılarak rotavirus ile coronavirusun belirlenmesi için Eylül 2001 ile Temmuz 2002 tarihleri arasında ishalli ve ishalsiz buzağılardan dışkı örnekleri toplandı. Neonatal buzağılarda ishal insidansı % 28.8 olarak bulundu. Neonatal buzağılarda rotavirus ve coronavirusun çiftlik prevalansının sırasıyla %31,1 ve %2,2 olduğu belirlendi. Rotavirus ve coronavirusun insidans oranlan (hayvan-yıl/100) tüm sürülerde sırasıyla 4,5 ve 0,2, etkilenen sürülerde ise 8,9 ve 2,3 olduğu bulundu. Coronavirus sadece bir ishalli buzağıda (%1) belirlenirken, rotavirus muayene edilen örneklerin % 19'unda bulundu. Rotavirus ishalli buzağıların % 26,9'unda ve ishalsiz buzağıların ise % 4,9'unda bulundu, ishalli olarak teşhis edilen buzağılarda rotavirus pozitivitesi daha yüksekti (Odds Ratio=6.8, P=0.008). Rotavirus yönünden pozitif vakaların yaşları 1-19 günler arasında değişti ve ortalama 6,5 günlüktü. Rotavirus çoğunlukla hayatın ilk haftası içinde görülürken, buzağılar yaşlandıkça rotavirus görülme oranı azaldı (p

Anahtar Kelimeler:

Coronavirus, ELISA, Rotavirus, yaş, buzağı, klinik muayene, ishal, hastalık yaygınlığı, lateks aglütinasyon testi, sürü büyüklüğü, yenidoğan hayvanlar, Kars

Kars yöresindeki neonatal buzağılarda rotavirus ve coronavirus yagınlığı

This study was aimed at determining the frequency of rotavirus and coronavirus in neonatal calves in Kars district. A two stage stratified random sampling strategy was used to select localities and farms. The study involved weekly visits to the farms and clinical examination of neonatal calves. Faecal samples were collected from diarrhoeic and non-diarrhoeic calves between September 2001 and July 2002 for the detection of rotavirus and coronavirus using latex agglutination and ELISA tests, respectively. Incidence of diarrhoea was 28.8% in neonatal calves. The farm prevalence -' of rotavirus and coronavirus in neonatal calves was determined to be 31.1% and 2.2%, respectively. The incidence rates (aniinal-year/100) of rotavirus and coronavirus were found to be 4.5 and 0.2 in all herds and 8.9 and 2.3 in affected herds, respectively. Coronavirus was detected in one diarrhoeic calf (1%) while rotavirus was found in 19% of samples examined. Rotavirus was present in 26.9% of diarrhoeic calves and in 4.9% non-diarrhoeic calves. Calves diagnosed as diarrhoeic were more likely to be positive for rotavirus (Odd Ratio=6.8, P=0.008). Mean age of rotavirus positive cases was 6.5 days with the range of 1-19. Rotavirus was the most common in the first week of life and as the calves grew older rotavirus positivity decreased (P<0.001). Calves born in crowded herds (P=0.03) or grouped in large numbers (P=0.01) were more likely to be positive for rotavirus and as the number of calves in the herd increased the risk of roiavirus infection also increased (P=0.009). The results indicate that rotavirus and coronavirus should also be considered when evaluating the aetiology and epidemiology of neonatal calf diarrhoea in Kars.

Keywords:

Coronavirus, ELISA, Rotavirus, age, calves, clinical examination, diarrhoea, disease prevalence, latex agglutination test, livestock numbers, newborn animals, Kars,

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