Mümtaz NAZLI

Immunocytochemical localisation of the orexn-A with calbindin D and nitric oxide synthase in the rat spinal cord

Öreksin (hipokretin) hipotalamusun lateralindeki sinir hücrelerinde sentezlenen, endokrin kontrol, enerji metabolizması ve besin maddesi alımını düzenleyen bir nöropeptitdir Bu çalışmanın amacî Wistar rat'ın omuriliğinde oreksin-A ile kalbindin D ve nöronal nitrik oksit sentetazm dağılımlarını immunoşitokimyasal metotla araştırmak ve mukayese etmektir. Orexin-A'nin immunoreaktif fibril ve sonlanmaları dorsal kornunun yüzeysel kısımlarında gözlendi. Kalbindin D ve nöronal nitrik oksit sentetaza ait sinir hücre ve uzantıları da aynı bölgede görüldü. Kalbindin D ve nöronal nitrik oksit sentetaza ait dorsal kornudaki hücre grupları oreksin-A fıbrilleriyle dorsal kornunun yüzeysel kısımlarında konsantre olmalarına rağmen, oreksin-A ile diğer iki sinir işaretleyicilerin birbirleriyle aynı sinir hücresinde v(ko-ekspres) veya anatomik olarak herhangi bir bağlantının nadiren olduğu belirlendi. Bu bulgular oreksinler ile kalbindin D ve nitrik oksitin besin maddesi alımı, nöroendokrin fonksiyonlar ve ağrı modulasyonu üzerine olan işlevlerinin bağımsız birtakım olaylar aracılığıyla yapıldığını gösterir. Bununla birlikte, nitrik oksit diffuze olabilen bir molekül olup, şinaptik olmayan mekanizmalar aracılığıyla öreksin nöronlarının aktivitelerini etkileyebilir. Dorsal kornunun yüzeysel kısımlarında küçük islet hücreleri halinde bulunan ve kalbindin D' yi ifade eden sinir hücreleri farklı bir grup olup, muhtemelen eksitatör ara nöronlardır.

Anahtar Kelimeler:

kalbidin, endokrinoloji, enerji metabolizması, hipotalamus, immünositokimya, sinir sistemi, sinir hücresi, nöropeptitler, nitrik oksit, beslenme, ağrı (tıp), sıçan, düzenleyiciler, omurilik

Ratların omuriliğinde oreksin-A ile kalbindin D ve nöronal nitrik oksit sentazının immunositokimyasal lokalizasyonları

Orexin (hypocretin) is synthesized by neurones in the lateral hypothalamus and is reported to regulate the food intake, energy metabolism and endocrine control. The aim of this study was to investigate and compare the distributions of orexin-A- with calbindin D- and neuronal nitric oxide synthase-containing neurones in the spinal cord of adult Wistar rats using immunocytochemistry. The orexin-A immunoreactive fibres and terminals are observed in the superficial dorsal horn. Calbindin D and neuronal nitric oxide synthase positive cells and processes are also distributed in the superficial dorsal horn. Although calbindin D and neuronal nitric oxide synthase cell populations with the orexin-A fibres are concentrated in the superficial dorsal horn, there was rarely co-localization or direct anatomic contact between orexin-A and other two markers in this region. These findings may suggest that the actions of the orexins with calbindin D and nitric oxide on the food intake, neuroendocrine functions and pain modulation are mediated via independent mechanisms. Thus nitric oxide is a diffusible molecule and could potentially affect the activity of orexin neurones via a non-synaptic mechanism. Calbindin D expressing neurons that were separate populations of small Islet cells in the superficial dorsal horn, are probably excitatory interneurons.

Keywords:

calbindin, endocrinology, energy metabolism, hypothalamus, immunocytochemistry, nervous system, neurons, neuropeptides, nitric oxide, nutrition, pain, rats, regulators, spinal cord,

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