Investigation of Toxin Genes of Staphylococcus aureus Strains Isolated from Gangrenous Mastitis in Ewes
Bu çalışmada, Şanlıurfa yöresinde, yedi koyun sürüsündeki gangrenli mastitis vakalarından izole edilen 110 Staphylococcus aureus suşlarında stafilakokal enterotoksin, toksik şok sendrom toksin ve eksfoliatif toksin genleri bakımından araştırılması amaçlandı. İncelenen suşlarda yalnızca sec ve tst toksin genleri saptandı. Bu çalışmanın sonucunda koyunlarda gangrenöz mastitislerden izole edilen S. aureus suşlarında enterotoksin üretiminin yüksek (%100) olduğu, izole edilen S. aureus suşlarının sec ve tst genlerini içerdiği ve bu genlerinde koyunlarda mastitisin patogenezisinde rol oynayabileceği kanısına varıldı.
Koyunlarda Gangrenli Mastitislerden İzole Edilen Staphylococcus aureus Suşlarının Toksin Genlerinin Araştırılması
In this study, it was aimed to determine staphylococcal enterotoxin (SE) genes, toxic shock syndrome toxin (TSST) gene and exfoliative toxin (ET) genes in 110 Staphylococcus aureus strains from gangrenous mastitis cases in seven ewe flocks in Sanliurfa, Turkey. Among investigated isolates, only sec and tst toxin genes were detected. The results of this study showed that the rates of enterotoxin production were high (100%) in S. aureus strains isolated from ovine gangrenous mastitis. Moreover, it was demonstrated that S. aureus causing gangrenous mastitis harboured sec and tst genes suggesting that these genes may have a role in ovine mastitis pathogenesis.
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