MESUT ÇEVİK, ALPER KOÇYİĞİT, UĞUR ŞEN, MEHMET KURAN

Can sequential human embryo culture media be used in bovine in vitro embryo culture?

Bu çalışmanın amacı, ticari insan embriyo kültür medyumlarının sığır embriyolarının kültüründe kullanılabilirliğinin araştırılmasıdır. Sığır oositleri, TCM-199’da 22 saat süreyle 38,5°C’de maturasyona tabi tutulduktan sonra, mTALP medyumunda fertilize edildiler. Muhtemel zigotlar rastgele 2 gruba ayrıldı; (1) sığır serum albümini (FAF-BSA) (8 mg/ml) ilaveli Sentetik Ovidukt Sıvısı (SOFaa) ve (2) sığır serum albümini (FAF-BSA) (8 mg/ml) ilaveli ardışık Quinn’s Advantage Medium (QAM). Muhtemel zigotlar SOFaa ve ardışık QAM medyumlarında 38.5°C’de 9 gün süre ile kültüre edildiler (%5 CO2, %5 O2 ve %90 N2). Yapılan istatistiksel değerlendirme sonucunda; bölünme oranı (%73.3 ve %72.2), morula (%37.6 ve %33.2) ve blastosiste ulaşma oranları yönünden (%23.9 ve %22.9) deneme grupları arasında önemli bir farklılık gözlenmemiştir (P>0.05). Buna karşın total blastosist hücre sayıları yönünden yapılan değerlendirmede, SOFaa medyumu (101.6±4.0) ve QAM medyumunda (87.4±3.2) kültüre edilen blastosistlerin hücre sayıları önemli oranda farklılık göstermiştir (P

Ardışık insan embriyo kültür medyumu sığır embriyolarının in vitro kültüründe kullanılabilir mi?

The aim of the study was to investigate potential use of sequential human embryo culture media in culture of bovine embryos. Bovine oocytes were matured in Tissue Culture Medium-199 (TCM-199) for 22 h at 38.5°C and fertilized in modified Tyrode-Albumine-Lactate- Pyruvate medium (mTALP). The putative zygotes were randomly allocated to two embryo culture media groups; (1) Synthetic Oviduct Fluid (SOF) supplemented with fatty-acid free bovine serum albumin (FAF-BSA) (8 mg/ml) and (2) sequential human embryo culture media [Quinn’s Advantage Medium-(QAM)] supplemented with FAF-BSA (8 mg/ml). Zygotes were cultured in SOF and sequential QAM for 9 days (5% CO2, 5% O2, and 90% N2) at 38.5°C. Cleavage (73.3% and 72.2%), morula (37.6% and 33.2%) and blastocysts rates (23.9% and 22.9%) were similar among groups (P>0.05), but the total blastocyst cell number were significantly higher in blastocysts developed in SOF (101.6±4.0) than those in sequential QAM (87.4±3.2) (P<0.05). QAM may be suggested to use in culture as an alternative media in terms of supporting embryo development, but low cell number in blastocysts produced in QAM may suggest a possible low pregnancy rate.

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