Effects of dietary boric acid and ascorbic acid supplementation on performance, some blood and bone parameters in broilers

Bu çalışma broyler rasyonlarına borik asit ve askorbik asit ilavesinin performans, bazı kan ve kemik parametreleri, karkas kalitesi ve MDA düzeyleri üzerine etkilerini belirlemek amacıyla gerçekleştirilmiştir. Araştırma 42 gün sürdürülmüştür. Araştırmada 16 bölmenin her birinde 15 adet günlük yaşta erkek 240 adet broyler civciv kullanılmıştır. Rasyonun temelini soya ve mısır oluşturmuştur. Bir kontrol ve 3 deneme grubu oluşturulmuş olup, kontrol grubuna herhangi bir ilave yapılmamıştır. Deneme grupları yemlerine ise sırasıyla 200 ppm askorbik asit, 175 ppm borik asit ve 200 ppm askorbik asit + 175 ppm borik asit ilavesi gerçekleştirilmiştir. Araştırma sonuçlarına göre; ilk 3 haftada kontrol grubu ve deneme grupları arasında canlı ağırlık, canlı ağırlık artışı ve yemden yararlanma oranı bakımından istatistiksel farklılıklar görülmüştür (P

Broyler rasyonlarına ilave edilen borik asit ve askorbik asidin performans, bazı kan ve kemik parametreleri üzerine etkileri

This study was carried out to determine the effects of boric acid (17.5% boron) and ascorbic acid supplementation on performance, selected blood and bone parameters, carcass yield and malondialdehid (MDA) levels of broilers. The experiment lasted in 42 days. Totally 240 one-day old male Ross 308 broilers were housed at a density of 15 chickhens in each of 16 experimental plots using a completely randomized block experimental design. Diets were based on maize and soybean meal. Experimental groups divided into one control (CON) and 3 experimental groups. The experimental diets supplemented with 200 ppm ascorbic acid (AA) for the first group, 175 ppm boric acid (BA) for the second group and 200 ppm ascorbic acid plus 175 ppm boric acid combination (AABA) for the third group. At the end of experiment period there were statistically significant differences (P<0.05) between control and the treatment groups about body weight, body weight gain and feed conversion ratio for first 3 weeks of the experimental period, but there were no statistically different for feed intake. Carcass yield increased with additives especially with boron supplementation (P<0.05). Total protein, cholesterol, trigliseride concentration and ALT activity were not affected by addition of feed additives but AST activity increased in BA group while it was reducing in AA and AABA groups. There were also statistically significant differences for left tibia P levels (P<0.05) and plasma, liver MDA levels (P<0.001). There were no statistically significant differences between control and the treatment groups for tibia ash, Ca levels and some strength parameters. As a result; it can be concluded that boric acid and ascorbic acid supplementation did have positive effect on performance in 0-21 days of the experimental period and also on MDA levels about lipid peroxidation activity, bone mineralization and hot carcass yield studied in the experiment. It is inferred that it will be usefull if these feed additives are studied again at different levels and under different conditions.

PDF

___

1. Yildiz G, Ozcelik F, Koksal BH, Bagder S, Abacioglu O: Organik bor üretilebilirliği ve broyler rasyonlarinda bor ile humatin kullanimi. 2. Ulusal Bor Çalıştayı, 17-18 Nisan, Ankara, Bildirileri Kitabı, s.597-604, 2008.
2. Cox C: Boric acids and borates. J Pes Ref, 2, 10-15, 2004.
3. Schauss AG: Boron. http://www.Traceminerals.com/products, Accessed: 17.04. 2005.
4. Naghii MR: The significance of dietary boron with particular reference to athletes. Nutr and Health, 13, 31-37, 1999.
5. Elliot MA, Edwards HM JR: Studies to determine whether an interaction exist among boron, calcium, and cholecalciferol on the skeletal development of broiler chickens. Poultry Sci, 71 (4): 677-90, 1992.
6. Rossi AF, Miles RD, Damron BL, Flunker LK: Effects of dietary boron supplementation on broilers. Poult Sci, 72, 2124-2130, 1993.
7. Murray FJ: A human health assessment of boron. Biol Trace Elem Res, 66, 331-341, 1998.
8. Cemeroglu B: Meyve ve Sebze İşleme Teknolojisi. 1. Cilt, 2. Baskı, Gıda Tekn. Der. Yayınları, 35, Ankara, s.55-60, 2004.
9. Yarsan E, Gulec M: Kanatlılarda stres, vitamin ve mineral uygulamaları. Türk Vet Hekim Birlik Derg, 3 (3-4): 55-63, 2003.
10. Zook EG: Total boron. J Assoc Agric Chem, 48, 850, 1965.
11. Sahin K, Sahin N, Kucuk O: Effects of chromium, and ascorbic acid supplementation on growth, carcass traits, serum metabolites, and antioxidant status of broiler chickens reared at a high ambient temperature (32°C). Nut Res, 23 (2): 225-238, 2003.
12. Erdogan Z, Erdogan S, Aksu T, Baytok E: The effects of dietary lead exposure and ascorbic acid on performance, lipid peroxidation status and biochemical parameters of broilers. Turk J Vet Anim Sci, 29, 1053-1059, 2005.
13. AOAC: Official Methods of Analysis of the Association of Official Analytical Chemists. 14th ed., Virginia, USA, 1990.
14. Türk Standartları Enstitüsü: Hayvan Yemleri - Metabolik (Çevrilebilir) Enerji Tayini (Kimyasal Metod). TSE No: 9610. TSE, Ankara, 1991.
15. Vanatta LE, Coleman DE, Slingsby RW: Low-level calibration study for a new ion chromatographic column to determine borate in deionized water. J Chromatogr A, 850, 107-117, 1999.
16. Karatepe M: Simultaneous determination of ascorbic acid and free molandialdehyde in human serum by HPLC-UV. LG CG Asia Pac, 22 (4): 362-365, 2004.
17. AOAC: Methods of Analysis of the Association of Official Analytical Chemists. 14th ed., Washington, DC, 1994.
18. Patterson PH, Cook ME, Crenshaw TD, Sunde ML: The bio- phosphonate alendronate (MK-217) inhibits bone loss due to overiectomy in rats. J Bone Miner, 6, 339-346, 1986.
19. Duncan DB: Multiple range and multiple F tests. Biometrics, 11, 1-42, 1995.
20. Sumbuloglu K, Sumbuloglu V: Biyoistatistik. 6. Baskı. Özdemir Yayıncılık, Ankara, 1995.
21. Fassani EJ, Bertechini AG, Brito JAG, Kato RK, Fialho ET, Geraldo A: Boron supplementation in broiler diets. Braz J Poult Sci, 6 (6): 213-217, 2004.
22. Yildiz G, Koksal BH, Abacioglu O: Rasyonlara ilave edilen maya ve borik asitin broylerlerde performans, karkas ve bazi kan parametreleri üzerine etkisi. Kafkas Univ Vet Fak Derg, 17 (3): 429-434, 2011.
23. Sahin K, Onderci M, Sahin N, Gursu MF, Kucuk O: Dietary vitamin C and folic acid supplementation ameliorates the detrimental effects of heat stres in Japenese quail. J Nutr, 133(6): 1882-1886, 2003.
24. Mizrak C, Bozkurt M, Kucukyılmaz K, Catli AU, Cinar M, Cabuk M, Bintas E: Etlik piliç karma yemlerine farkli düzeylerde bor ilavesinin performans, kemik gelişimi ile kalsiyum metabolizmasi üzerine etkilerinin belirlenmesi. BOREN-2006-46-G12-12 Kodlu Proje Raporu, 2007.
25. Koksal BH, Yildiz G, Sizmaz O: Effects of boric acid and humate supplementation on some performance and egg quality parameters of laying hens. Braz J Poultry Sci,14 (4): 283-289, 2012.
26. Mizrak C, Ceylan N: Damızlık yumurta tavuğu yemlerine farklı seviye ve formda bor ilavesinin performans, kemik gelişimi ve bazı kan parametreleri üzerine etkisi. V. Ulusal Hayvan Besleme Kongresi, 30 Eylül-03 Ekim, Tekirdağ/Çorlu, Tam Metinler Kitabı, s.130-138, 2009.
27. Kurtoglu F, Kurtoglu V, Celik-Kececi T, Nizamlioglu M: Effects of dietary boron supplementation on some biochemical parameters, peripheral blood lymphocyte, splenic plasma cell counts and bone characteristics of broiler chicks fed with adequate or inadequate vitamin D3 containing diet. Br Poultry Sci, 46 (1): 87-96, 2005.
28. Eklin RG, Freed M, Watkins BA, Srebnik M, Kieft KA, Newton RS: Evaluation of two novel biochemicals on plasma and egg yolk lipid composition and laying hen performance. Poult Sci, 72, 513-520, 1993.
29. Eren M, Kocaoglu Guclu B, Uyanik F, Karabulut N: The effects of dietary boron supplementation on performance, carcass composition and serum lipids in Japanese quails. JAVA, 5 (12):1105-1108, 2006.
30. Eren M, Uyanik F: Influence of dietary boron supplementation on some serum metabolites and egg-yolk cholesterol in laying hens. Acta vet Hung, 55 (1): 29-39, 2007
31. Gursu MF, Onderci M, Gulcu F, Sahin K: Effects of vitamin C and folic acid supplementation on serum paraxonase activity and metabolites induced by heat stress in vivo. Nutr Res, 24, 157-164, 2004.
32. Erdogan Z, Erdogan S, Celik S, Unlu A: Effects of ascorbic acid on cadmium-induced oxidative stress and performance of broilers. Biol Tr Elem Res, Abstr., 104 (1): 19-32, 2005.
33. Mizrak C, Yenice E, Can M, Yildirim U, Atik Z: Effects of dietary boron on performance, egg production, egg quality and some bone parameters in laying hens, South African. J Anim Sci, 40 (3): 257-261, 2010.
34. Wilson JH, Ruszler PL: Long term effcts of boron on layer bone strength and production parameters. Br Poult Sci, 39 (Abstract), 1998.
35. Dogan V, Bahiiyarca Y: Japon bıldırcınlarında inorganik bor kaynaklarının nispi biyolojik kullanilabilirliği konusunda bir araştırma. 2. Ulusal Bor Çalıştayı, 17-18 Nisan, Ankara, Bildiriler Kitabı, s.619-625, 2008.
36. Kurtoglu V, Kurtoglu F, Coskun B: Effects of boron supplementation of adequate and inadequate vitamin D3-containing diet on performance and serum biochemical characters of broiler chickens. Res Vet Sci, 71, 183- 187, 2001.
37. Konca Y, Kirkpinar F, Yaylak E, Mert S: Effect of dietary ascorbic acid on performance, carcass composition and bone characteristics of turkeys during high summer temperature. AJAS, 21 (3): (Abstr.), 2008.
38. Sahin K, Sahin N, Yaralioglu S: Effects of vitamin C and vitamin E on lipid peroxidation, blood serum metobolites and mineral concentrations of laying hens reared at high ambient temperature. Biol Tr Elem Res, 85, 35-45, 2002.
39. Sahin K, Onderci M, Sahin N, Gursu MF, Gursu MF: Ascorbic acid and melatonine reduce heat-induced performance inhibition and oxidative stres in Japanese quails. Br Poult Sci, 45 (1): 116-122, 2004.
40. Karabulut-Bulan O, Bolkent S, Yanardag R, Bilgin-Sokmen B: The role of vitamin C, vitamin E and selenium on cadmium-induced renal toxicity of rats. Drug Chem Toxicol, 31, 413-426, 2008.
41. Seven İ, Tatlı-Seven P, Yılmaz S: Responses of broilers under cold conditioning (15oC) to dietary triiodothyronine and iodine combined to antioxidants (selenium and vitamin C). Kafkas Univ Vet Fak Derg, 15 (4): 499-504, 2009.
42. Rossi AF: Effect of boron supplementation of practical corn, soybean meal diets for poultry. PhD Dissertation, University of Florida, 1990.
43. Ozkurt S: Çatören ve Kunduzlar (Kırka - Eskişehir) baraj göletlerindeki sazanlarin (Cyprinus carpio L., 1758) dokularinda bor birikimi. Türk J Biol, 23, 663-676, 2000.