MUZAFFER TAŞ, ÜMÜT CİRİT, OKTAY ÖZKAN, MUZAFFER DENLİ, SEYİT BURHANEDTİN ZİNCİRCİOĞLU, Uğur ŞEKER, MEHMET FERİT ÖZMEN, Leyla EREN BİLGE

Protective role of vitamin c on sperm characteristics and testicular damage in rats exposed to radiation

Bu çalışmada, vitamin C’nin gamma radyasyon (2 Gy) uygulanmış erkek sıçanlarda spermatolojik özellikler ve testis üzerine olan koruyucu etkilerini saptamak amaçlandı. Çalışmada, 8 haftalık yaşta ve ortalama 180-220 g canlı ağırlığa sahip toplam 21 adet Wistar Albino ırkı erkek sıçanlar kullanıldı. Sıçanlar rastgele 3 eşit gruba ayrıldı. Gruplar; Kontrol, radyasyon tedavi (scrotal bölgeye tek doz 2 Gy radyasyon ışıması yapıldı) ve radyasyon tedavi + vitamin C grubu (radyasyon ışımasından sonraki 55 gün boyunca, günlük oral olarak, 500 mg/500 ml, vitamin C/su) şeklinde oluşturuldu. Tüm gruplardaki sıçanlardan radyasyon tedavisi sonrası 55. gününde genital organlar alınarak spermatolojik özellikler, genital organ ağırlıkları ile testis dokusuna ait histolojik özellikler değerlendirildi. Radyasyon tedavisi ve kontrol grubu, spermatolojik, morfometrik ve histolojik özellikleri bakımından karşılaştırıldığında, Radyasyon tedavisi uygulanan hayvanların motilite, konsantrasyon, sol testis ağırlığı, epididimis ağırlığı, seminifer tubul çapı ve Jhonsen biyopsi skorlarında istatistiksel olarak önemli düzeyde azalma ve anormal spermatozoon baş sayısında ise artış saptandı (P

Radyasyona maruz bırakılmış sıçanlarda spermatozoa özellikleri ve testislerde yarattığı hasar üzerine vitamin c’nin koruyucu rolü

The aim of this study was to investigate the protective effects of vitamin C on sperm characteristics and testes in male rats exposed gamma radiation (2 Gy). A total of 21 adult male wistar albino rats (8 weeks of age, weighing 180-220 g) were divided into three groups. Control, radiotherapy (received scrotal γ-radiation of 2 Gy as a single dose) and radiotherapy + vitamin C treated rats (during the 55 days after irradiation, 500 mg vitamin C/500 ml water daily orally). Testes samples from all groups were taken at day 55 post-irradiation and epididymal sperm characteristics, all-genital organs weights and testes histology were evaluated. Radiotherapy decreased significantly the sperm motility, concentration, left testes and epididymis weights, Johnsen’s biopsy score and seminiferous tubular diameter but it increased the sperm head defects as compared to the Control group (P<0.05). The administration of vitamin C only reduced the harmful effects of radiotherapy on the seminiferous tubular diameter (P<0.05). It has been concluded that the radiotherapy may cause alteration in the genital organ weights, spermatologic and hystologic parameters in rats and administration of vitamin C may be slightly beneficial for seminiferous tubular diameter following testicular irradiation during the radiotherapy.

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