ABDULLAH DOĞAN, PINAR AKSU KILIÇLE, DİNÇER ERDAĞ, Kadir ÖZCAN, Ertan DOĞAN

Farelerde 3-Metilkolantrenle indüklenen fibrosarkoma üzerine Sisteamin, Putresin ve Sisteamin-Putresin kombinasyonunun etkileri

Bu çalışmada farelerde 3-metilkolantren (3-MC) ile indüklenen fibrosarkoma üzerine sisteamin, putresin ve sisteamin-putresin kombinasyonunun etkileri araştırıldı. Araştırmada Mus musculus albino ırkı, 2-3 aylık ve 20±2.0 g ağırlığında olan toplam 135 adet erkek fare kullanıldı. Fareler her grupta 15 adet olacak şekilde 9 gruba ayrıldı. Fareler standart diyet ve su ile ad libitum olarak beslendi. Birinci grup negatif kontrol grubu olarak tutuldu. İkinci gruba deri altı yolla 0.2 ml susam yağı, üçüncü gruba deri altı yolla 3-MC enjekte edildi. Dördüncü gruba içme suyuyla %0.1 oranında sisteamin, beşinci gruba %0.1 oranında putresin, altıncı gruba %0.1 oranında sisteamin ve %0.1 oranında putresin karışımı ad libitum olarak verildi. Yedinci gruba deri altı yolla 0.2 ml 3-MC çözeltisi (1 mg 3-MC/0.2 ml susam yağı) ve bir ay sonra içme suyuyla %0.1 oranında sisteamin, sekizinci gruba deri altı yolla 0.2 ml 3-MC çözeltisi ve bir ay sonra içme suyuyla %0.1 oranında putresin, dokuzuncu gruba deri altı yolla 0.2 ml 3-MC çözeltisi ve bir ay sonra içme suyuyla %0.1 oranında sisteamin + %0.1 oranında putresin karışımı ad libitum olarak verildi. Bir yılın sonunda ferelerin dokuları morfolojik ve histopatolojik olarak değerlendirildi. Araştırma sonucunda 3-MC ile indüklenen fibrosarkomaya karşı çoktan aza doğru putresin, sisteamin+putresin ve sisteaminin koruyucu etki gösterdiği belirlendi.

Effects of Cysteamine, Putrescine and Cysteamine-Putrescine Combination on 3-Methylcholanthrene-Induced Fibrosarcoma in Mice

In this study the effects of cysteamine, putrescine and the combination of cysteamine and putrescine were investigated in mice with 3-Methylcholanthrene (3-MC) induced on fibrosarcoma. A total of 135 adult male Mouse (Mus musculus) albino, 2-3 months old and weighting 20±2.0 g was used in this study. Mice in each group were divided 15 consisting of 9 individual. The first group was kept as a negative control group. The second group received subcutaneous injection of 0.2 ml sesame oil, and the third group was given subcutaneously 3-MC (1 mg/0.2 ml sesame oil). The 4th, 5th and 6th groups recived 0.1% cysteamine, 0.1% putrescine and 0.1% cysteamine + 0.1% putrescine mix with drinking water ad libitum respectively. The 7th, 8th and 9th groups were injected with 0.2 ml of 3-MC solution. After 1 month 0.1% cysteamine, 0.1% putrescine and 0.1% cysteamine + 0.1% putrescine combinations were administrated in drinking water to 7th, 8th and 9th groups respectively. After 1 year of all experiments mice tissues were evaluated morphologic and histopathologically. As a result were respectively demonstrated protective effect of putrescine, cysteamine+putrescine and cysteamin against 3-MC induced fibrosarcoma.

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