CİHAN KAÇAR, NADİDE NABİL KAMİLOĞLU, ÖMER UÇAR, UMUT ÇAĞIN ARI, ŞÜKRÜ METİN PANCARCI, ÖRSAN GÜNGÖR

İneklerde B-karoten + E vitamini uygulamasıyla kombine edilen Ovsynch ve cosynch senkronizasyon programlarının gebelik oranı üzerine etkisi

Özet Sunulan çalışmanın amacı, ineklerde P-karoten+Vitamin E (Dalmavital®) uygulamasıyla kombine edilen Ovsynch ve Cosynch senkronizasyon programları yardımıyla suni tohumlama sonrası gebelik oranını artırmaktır. Değişik ırk ve yaşlardaki toplam 225 baş inek 4 deneme grubuna ayrıldı: "P-karoten+Vitamin E+Ovsynch" (Grup I, n=81), "P-karoten+ Vitamin E+Cosynch" (Grup II, n=45), "Ovsynch" (Grup III, n=60) ve "Cosynch" (Grup IV, n=39). Tüm ineklerde prematüre östrus oranı (Grup l'de en yüksek, %18.5; p0.05) daha yüksekti. Buna karşın, gebelik durumlarına bakılmaksızın ve özellikle PGF2a enjeksiyonu öncesi, Grup I ve ll'deki plazma p-karoten düzeyleri (43.7±34.8 ile 64.9±47.2 jag/dl arası), Grup III ve IV'teki düzeylere (18.1 ±1.3 ile 19.9±5.9 ju.g/dl arası) göre önemli ölçüde (p

Anahtar Kelimeler:

GnRH, suni tohumlama, beta-karoten, inek, gebelik, eş zamanlama, E vitamini

Effect of Ovsynch and cosynch synchronisation programmes combined with B-carotene+vitamin E administration upon the pregnancy rates in cows

Summary The aim of the present study was to increase the pregnancy rates following artificial insemination of cows by using Ovsynch and Cosynch synchronisation programmes combined with P-carotene+Vitamin E (Dalmavital®) injection. A total of 225 cows in varying ages and breeds were divided into 4 trial groups as follows: "P-carotene+Vitamin+Ovsynch" (Group I, n=81), "P-carotene+Vitamin+Cosynch" (Group II, n=45), "Ovsynch" (Group III, n=60) and "Cosynch" (Group IV, n=39). For all the cows, the total premature oestrus rate (being the highest, 18.5% in Group I; p<0.001) was 9.3% (21/225) and these 21 cows were then excluded from the calculations of p-carotene level and pregnancy rate. Considering the 204 cows remained, the resultant pregnancy rates (25.8% and 34.1%) in Groups I (n=66) and II (n=44) were relatively (p>0.05) higher than those rates (17.5% and 21.6%) in Groups III (n=57) and IV (n=37), respectively. By contrast, regardless of the presence of pregnancy and before the PGF2oc injection in particular, P-carotene levels (ranging from 43.7±34.8 to 64.9± 47.2 u.g/dl) in Groups I and II were significantly (p<0.001) higher than those rates (ranging from 18.1 ±1.3 to 19.9±5.9 (ig/dl) in Groups III and IV, respectively. In conclusion, it was suggested that P-carotene+Vitamin E administration combined with Ovsynch and Cosynch synchronisation programmes may relatively increase the pregnancy rates following artificial insemination and hence, the supplementary administration of P-carotene+Vitamin E to the synchronisation protocol would be useful, at least to some extent, for achieving a higher fertility during the postpartum period in cows.

Keywords:

GnRH, artificial insemination, beta-carotene, cows, pregnancy, synchronization, vitamin E,

PDF

___

1. Schweigert Fj: (3-Carotin-Stoffwechsel des Rindes und seine Bedeutung für die Fruchtbarkeit. Übers. Tierernâhrung, 16, 223-246, 1988.
2. Lothammer KH, Ahlswede L, Meyer H: Untersuchungen über eine spezifische Vitamin-A unhabhângige Wirkung des 3-Carotins und die Fertilitât des Rindes. 2. Mitteilung: Weitere klinische Befünde und Befruchtunsergebnisse. Dtsch Tierârztl Wschr, 83, 353-358, 1976.
3. Zerobin K: Physiologic der Fortpflanzung. in, Scheunert A, Trautmann A (Eds): Lehrbuch der Veterinârphysiologie. 7. Auflage, Verlag Paul Parey, 215-221, 1987.
4. Haliloğlu S, Baspıhar N, Serpek B, Erdem H, Bulut Z: Vitamin A and P-carotene levels in plasma, corpus luteum and follicular fluid of cyclic and pregnant cattle. Reprod Dom Anim, 37,96-99,2002.
5. Lothammer KH: Importance of P-carotene fort he fertility of female cattle. F Hoffmann- La Roche und Co. Ltd. Basle. 1-25,1981.
6. Peltier MM, Peltier MR, Sharp DC, Ott EA: Effect of pcarotene administration on reproductive function of horse and pony mares. Theriogenology, 48, 893-906, 1997.
7. Pusateri AE, Diekman M, Singleton A: Failure of vitamin A to increase litter size in sows receiving injections at various stages of gestation. )Anim Sci, 77,1532-1535,1998.
8. De Kruif A, Mansfeld R, Hoedemaker M: Tierârztliche Bestandsbetreung beim Milchrind. Enke Verlag, Stuttgart, 1998.
9. Pursley JR, Mee MO, Wiltbank MC: Synchronization of ovulation in dairy cows using PGF2 alpha and GnRH. Theriogenology, 44, 915-923, 1995.
10. Geary TW, Whittier JC: Effect of a timed insemination following synchronization of ovulation using the Ovsynch or Co-Synch protocol in beef cows. Prof Anim Sci, 14, 217-220,1998.
11. Edmonson AJ, Lean IJ, Weaver LD, Farver T, Webster G: A body condition scoring chart for Holstein dairy cows. J Dairy Sci, 72, 68-78, 1989.
12. Suzuki JI, Katoh N: A simple and cheap methods for measuring serum vitamin A in cattle only a spectrophotometer. JpnJ Vet Sci, 52, 1281-1283, 1990.
13. Rosenberg M, Kaim M, Herz Z, Folman Y: Comparison of methods for synchronization of estrous cycle in dairy cows. 1. Effect on plasma progesterone and manifestation of estrus. J Dairy Sci, 73, 2807-2816, 1990.
14. Me Millan KL, Henderson HV: Analyses of the variation in the interval of prostaglandin F2 alpha to oestrus as a method of studying patterns of follicle development during dioestrus in dairy cows. Anim Reprod Sci, 6, 245- 254,1984.
15. Heuweiser W, Mansfeld R: Ostrus synchronisation. In, Grunert E, De Kruif A (Eds): Fertilitâtsstörungen beim weiblichen Rind. 3. Auflage, Parey Verlag, Berlin, pp 351-358,1999.
16. Bindaş EM, Aillo RJ, Gwazdauskas FC, Herbeein JH, Polan CE: Effect of P-carotene supplementation on reproductive and metabolic parameters in dairy cattle. J Dairy Sci, 65, 212-216, 1982.
17. Hemken RW, Bremel DH: Possible role of beta-carotene in improving fertility in dairy cattle. J Dairy Sci, 65, 1069- 1073,1982.
18. Coffey MT, Britt JH: Enhancement of sow reproductive performance by P-carotene or vitamin A. J Anim Sci, 71, 1198-1202,1993.
19. Özpınar H, Şenel HS, Özpınar A, Çekgül E: İneklerde döl verimi ile serumdaki P-carotene, A ve E vitamin düzeyleri arasındaki ilişkiler. Doğa Türk Vet Hay Derg, 13, 273- 282, 1998.
20. Smith MW: The use of p-carotene in dairy rations. 13th Ann. Conference Am. Assoc. Bovine Practitioners. November 22, Toronto, Ontario, Canada, 1980.
21. Akonder FBY, Stone JB, Walton JS, Buchanon-Smith jG: The role of P-carotene in the fertility of dairy cattlemaintained in a confined environment. J Dairy Sci, 67, 148/1984.
22. Bonomi A, Guarentelli A, Sabbioni A, Superchi P: Inclusion of protected (5-carotene in diets for dairy cows. Rivista SociItal, 23, 233-249, 1993.
23. Dryanvski D, Tsvetkova V, Goncharova I, Simeonov S: Effect of vitamin A and P-carotene on reproduction in cows. VetSbirka, 86, 44-46, 1989.
24. Kaçar C, Yildiz S, Pancarci SM, Kaya M, Oral H, Gurbulak K, Gungor O: Administration of GnRH treatment prior to ovsynch protocol to stimulate cyclicity in cows with functional anoestrus. Bull Vet Inst Puiawy, 50,497-501,2006.
25. Seguin B: Ovsynch: A method for breeding dairy cows without doing heat detection. Bovine Pract, 31,11-14, 1997.
26. Klindworth HP, Hoedemaker M, Burfeindt D, Heilkenbinker T: Ovulations synchronisation (ovsynch) in hochleistenden Milchviehherden: I. Fruchbarkeitsparameter, Body Condition Score und Plasma-Progesteron- Konzentration. Dtsch Tierârztl Wschr, 108, 11-19, 2001.
27. Sellars CB, Dalton JC, Manzo R, Day J, Ahmadzadeh A: Time and incidence of ovulation and conception rates after incorporating estradiol cypionate into a timed artificial insemination protocol. J Dairy Sci, 89, 620-626, 2006.
28. Zi XD, He SM, Lu H, Feng jA, Lu jY, Chang S, Wang X: Induction ofestrus in suckled female yaks (Bos grunniens) and synchronization of ovulation in the non-sucklers for timed artificial insemination using progesterone treatments and co-synch regimens. Anim Reprod Sci, 92, 183-192,2006.
29. Peckelhoff H, Kuchenbuch S, Künhe S, Biedermann A, Heuweiser W: Fruchtbarkeitsmanagement bei Fârsen durch Ovulationssynchronisation. Tierârztl Prax, 28, 88- 92, 2000.
30. Nak Y, Nak D, Karakaş E: Siklik ve asiklik anöstrüslu ineklerde ovsynch ve ovsynch+cosynch uygulamalarının gebelikler üzerine etkilerinin karşılaştırılması. Uludağ Üniv Vet Fak Derg, 24, 41 -46, 2005.
31. Dejarnette JM, Day ML, House RB, Wallece RA, Marshall CE: Effect of GnRH pretreatment on reproductive performance of postpartum suckled beef cows following synchronization ofestrus using GnRH and PGF2a. J Anim Sci, 79, 1675-1682, 2001.
32.Willard S, Gandy S, Bowers S, Graves K, Elias A, Whisnant C: The effects of GnRH administration post insemination on serum concentrations of progesteron and pregnancy rates in dairy cattle exposed to mild summer heat stress. Theriogenology 59, 1799-1810, 2003.