AZİZ BÜLBÜL, Tuğba BÜLBÜL, SEHER KÜÇÜKERSAN, Meltem ŞİRELİ, ABDULLAH ERYAVUZ

Effect of dietray supplementation of organic and inorganic Zn, Cu and Mn on oxidant/antioxidant balance in laying hens

Bu araştırma, yumurta tavuğu rasyonlarına organik ve inorganik çinko, bakır ve manganez katılmasının plazma malondialdehit (MDA), nitrik oksit (NO), glutasyon (GSH) ve antioksidan aktivite (AOA) üzerine etkisini belirlemek amacıyla yapılmıştır. Araştırmada 60 haftalık toplam 280 beyaz yumurta tavuğu (Lohmann LSL) kullanılmıştır. Araştırma her biri 40 tavuktan oluşan biri kontrol ve altı deneme grubu olmak üzere yedi grup halinde yürütülmüştür. Her grup 10 tavuk içeren 4 tekrar grubuna ayrılmıştır. Kontrol ve deneme grupları rasyonları ortalama %17 ham protein ve 2750 kcal/kg metabolize olabilir enerji içerecek şekilde düzenlenmiştir. Deneme gruplarından inorganik mineraller ilave edilenlere bu minerallerin oksit, organik ilave edilenlere ise proteinat formları eklenmiştir. Çinko oksit, Zn-proteinat, CuO, Cu-proteinat, MnO ve Mn-proteinat gruplarının yemlerine sırasıyla; ZnO 50 mg/kg, Zn-proteinat 10 mg/kg, CuO 200 mg/kg, Cu-proteinat 50 mg/kg, MnO 30 mg/kg ve Mn-proteinat 5 mg/kg ilave edildi. Araştırma sonunda organik ve inorganik Zn'nun plazma MDA ve NO düzeylerini azalttığı, AOA'yı değiştirmediği buna karşın Zn-proteinatın plazma GSH'ı artırdığı; CuO'in MDA düzeyini düşürürken NO, GSH ve AOA'yı değiştirmediği; Cu-proteinatın MDA düzeyini artırırken GSH ve AOA'yı azalttığı NO etkilemediği; MnO ve Mn-proteinatın plazma MDA düzeyini azaltırken NO, GSH ve AOA düzeylerini değiştirmediği belirlendi. Sonuç olarak organik ve inorganik Zn ve Mn'in yumurtacı tavuklarda oksidatif stresi baskılarken özellikle organik bakırın artırdığı belirlenmiştir.

Anahtar Kelimeler:

yem katkı maddeleri, yumurta tavuğu, lipit peroksidasyonu, mangan, çinko, nitrik oksit, antioksidanlar, bakır

Yumurtacı tavuklarda yeme organik ve inorganik Zn, Cu ve Mn ilavesinin oksidan/antioxsidan denge üzerine etkileri

Effects of organic and inorganic zinc, copper and manganese supplementations into the diet on plasma malondialdehyde (MDA), nitric oxide (NO), glutation (GSH) and antioxidant activity (AOA) were investigated in totally, 280 white strain laying hens (Lohmann LSL), aged 60 weeks. The experimental animals were divided into 7 different groups as 6 experimental and 1 control groups consisted of 40 laying hens in each group. Per group also divided into four subgroup containing 10 laying hens. The diets of the control and experimental groups contained mean 17% crude protein and 2750 kcal/kg metabolizable energy. The proteinate forms were used as organic sources of Zn, Cu and Mn, whereas the oxide forms were used as inorganic sources of Zn, Cu and Mn. 50 mg/kg of ZnO, 10 mg/kg of Znproteinate, 200 mg/kg of CuO, 50 mg/kg of Cu-proteinate, 30 mg/kg of MnO, 5 mg/kg of Mn-proteinate were added into the basal diets of ZnO, Zn-proteinate, CuO, Cu-proteinate, MnO and Mn-proteinate groups, respectively. Both organic and inorganic sources of Zn decreased plasma MDA and NO concentrations, while AOA remained unchanged, and whereas Zn-proteinate increased GSH concentration. CuO decreased the plasma MDA concentration but it did not alter the plasma NO, GSH and AOA. Cu-proteinate increased the plasma MDA concentration but decreased GSH and AOA. Both MnO and Mn-proteinate decreased the plasma MDA concentration and unchanged the plasma NO, GSH and AOA. It was concluded that both organic and inorganic sources of zinc and Mn decreased the oxidative stress in the laying hens, whereas especially organic copper increased this effect.

Keywords:

feed additives, hens, lipid peroxidation, manganese, zinc, nitric oxide, antioxidants, copper,

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REFERENCES 1. Mabe I, Rapp C, Bain MM, Nys Y: Supplementation of a corn-soybean meal diet with manganese, copper and zinc from organic or inorganic sources improves eggshell quality in aged laying hens. PoultSci, 82,1903-1913, 2003. 2. Szentmihâlyi K, Blâzovics A, Vinkler P: Free radical properties of metal complexes. Acta Biologica Szegediensis, 1,107-109,2003. 3. Close WH: Biotechnology in the Feed Industry, Proceedings of Alltech's 15th Annual Symposium. Nottingham University Press, England, 1999. 4. Şahin N, Onderci M, Şahin K: Effects of dietary chromium and zinc on egg production, egg quality, and some blood metabolites of laying hens reared under low ambient temperature. Biol Trace Elem /?es, 85, 47-58, 2002. 5. Halliwell B, Gutteridge JM: Lipid peroxidation, oxygen radicals, cell demage and disease. Biochem J, 219, 1-14, 1984. 6. Jia-Perng JW, Chandra S, Holly H, Joan SV, Edith BG: Evidence for a novel role of copper-zinc superoxide dismutase in zinc Metabolism. J Biol Chem, 276, 44798- 44803,2001.7. Kraus A, Roth HP, Kirchgessner M: Supplementation with vitamin C, vitamin E or (3-carotene influences osmotic fragility and oxidative damage of erythrocytes of zincdeficient rate. JNutr, 127, 1290-1296, 1997. 8. Prasad AS, Bao B, Beck FW, Kucuk O, Sarkar FH: Antioxidant effect of zinc in humans. Free Radic Biol Med, 37, 1182-90, 2004. 9. Shaheen AA, El-Fattah AA: Effect of dietary zinc on lipid peroxidation, glutathione, protein thiols levels and superoxide dismutase activity in rat tissues. IntJ Biochem Cell Biol, 27, 89-95, 1995. 10. Sahin K, Smith MO, Onderci M, Sahin N, Gursu MF, Kucuk O: Supplementation of zinc from organic or inorganic source improves performance and antioxidant status of heat-distressed quail. Poult Sci, 84, 882-887, 2005. 11. Uriu-Adams JY, Keen CL: Copper, oxidative stres, and human health. Molecular Aspects of Medicine, 26, 268-298, 2005. 12. Robinson, BH: The role of manganese superoxide dismutase in health and disease. J Inher Metab Dis, 21, 598-603, 1998. 13. Klasing KC: Comparative avian nutrition. University pres at Cambridge UK, 1998. 14. inal F, Coşkun B, Gülsen N, Kurtoglu V: The effects of withdrawal of vitamin and trace mineral supplements from laying hens diets on egg yield and trace mineral composition. Br Poultry Sci, 42, 77-80, 2001. 15. Draper HH, McGirr LG, Hadley M: The metabolism of malondialdehyde. Lipids, 21, 305-307, 1986. 16. Beutler E, Gelbart T, Pegelow C: Erythrocyte glutathione synthetase deficiency leads not only to glutathione but also to glutathione-S-transferase deficiency. J Clin Invest, 77, 38-41, 1986. 17. Koracevic D, Koracevic G, Djordjevic V, Andrejevic S, Cosic V: Method for measurement of antioxidant activity in human fluids. J Clin Path, 54, 356-361, 2001. 18. Miranda KM, Espey MG, Wink AD: A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric Oxide, 5, 62-71, 2001. 19. Eryavuz A, Dündar Y, Aslan R, Cengiz N: Investigation of the blood glutathione and malondialdehyde levels in tannery workers. Kocatepe Tip Derg, 2, 147-151, 2001. 20. Aydemir T, Ozturk R, Bozkaya LA, Tarhan L: Effects of antioxidant vitamins A, C, E and trace elements Cu, Se on CuZn SOD, GSH-Px, CAT and LPO levels in chicken erythrocytes. Cell Biochem Funct, 18, 109-15, 2000. 21.Ozturk-Urek R, Bozkaya LA, Tarhan L: The effects of some antioxidant vitamin- and trace elementsupplemented diets on activities of SOD, CAT, GSH-Px and LPO levels in chicken tissues. Cell Biochem Funct, 19,125-32,2001. 22. Zhang SS, Noordin MM, Rahman SO, Haron J: Effects of copper overload on hepatic lipid peroxidation and antioxidant defense in rats. Vet Hum Toxicol, 42, 261- 264, 2000. 23. Brian J: Catalytic antioxidants: A radical approach to new therapeutics. Therapeutic Focus, 9, 557-566, 2004. 24. Trostchansky A, Ferrer-Sueta G, Batthy'Any C, Botti H, Batini'C-Haberle I, Radi R, Rubbo H: Peroxynitrite fluxmediated LDL oxidation is inhibited by manganese porphyrins in the presence of uric acid free radical biology. Medicine, 35, 1293-1300, 2003. 25. Förstermann U, Schmidt HHHW, Pollock JS, Sheng H, Mitchell jA, Warner TD, Nakane M, Murad F: Isoforms of nitric oxide synthase characterization and purification from different cell types. Bioc Pharmacol, 42, 1849-1857, 1998. 26. David R, Janero DR: Nutritional aspects of nitric oxide: human health implications and therapeutic opportunities. Nutrition, 17, 896-903, 2001. 27. Bianchini A, Giovanni M, Lilia C: Inhibition of endothelial nitric oxide synthase by ceruloplasmin. J Biol Chem, 77A, 20265-20270, 1999. 28. Gaetke LM, Chow CK: Copper toxicity, oxidative stress and antioxidant nutrients. Toxicology, 189, 147-162, 2003. 29. Pfeiffer S, Schrammel A, Koesling D, Schmidt K, Mayer B: Molecular actions of a Mn(lll)porphyrin superoxide dismutase friimetic and peroxynitrite scavenger: reaction with nitric oxide and direct inhibition of NO synthase and soluble guanylyl cyclase. Mol Pharmacol, 53, 795-800, 1998. 30. Reid GM, Tervit H: Sudden infant death syndrome: oxidative stress. Medical Hypotheses, 52, 577-580, 1999.