MAHMUT ÜLGER, Seda TEZCAN ÜLGER, Efdal OKTAY GÜLTEKİN, ERDAL YABALAK, Harun GÜLBUDAK, Nuran DELİALİOĞLU, Ahmet Murat GİZİR, Gönül ASLAN

In vitro Antileishmanial Eff ect of the Plant Extracts from Aloe vera (L.) Burm.f. and Hypericum perforatum L. Leaves

The activity of currently available antiparasitic drugs has been threatened by the occurrence of drug-resistant parasite populations, toxic eff ects,and high cost. Therefore, the discovery of more potent antiparasitic drugs coming from medicinal plants is seen as a signifi cant approach toovercome the problem. This study aimed to evaluate the in vitro effi ciency of plant extracts of Aloe vera (L.) Burm.f. and Hypericum perforatumL. leaves against promastigote forms of Leishmania tropica. The antileishmanial activity of the plant extracts was determined using in vitromicrodilution method. Decreasing concentrations (25 to 0.01 mg/mL) of extracts were tested on Leishmania promastigotes. The eff ect of plantextracts on the viability of promastigotes of L. tropica was evaluated by counting viable or motile forms in a Neubauer hemocytometer. Thedata assessed as % growth in comparison to the controls. The 50% inhibitory concentration (IC50) values of the plant extracts were determinedusing The Quest Graph™ IC50 Calculator by logistic regression analysis. A. vera (L.) Burm.f. showed leishmanicidal activity at high concentrationsof 25, 12.5, and 6.25 mg/ml with 100% growth inhibition of L. tropica promastigotes, while H. perforatum L. was found to be eff ective atthe concentration range of 25 to 1.56 mg/mL. The IC50 of H. perforatum L. was determined as 0.23 mg/mL, and IC50 of A. vera (L.) Burm.f wasdetermined as 1.91 mg/mL. Our study showed that A. vera (L.) Burm.f. and H. perforatum L. leaves can be a potential medicinal alternatives for thetreatment of Leishmaniasis. The antiparasitic effi ciency of these plant extracts can be considered a signifi cant improvement in the specifi cationof antileishmanial agents and should be supported by further in vivo studies.

Aloe vera (L.) Burm.f. ve Hypericum perforatum L. Yapraklarından Elde Edilen Bitki Ekstraktlarının İn vitro Antileishmanial Etkisi

Günümüzde mevcut antiparazitik ilaçların etkinliği, ilaca dirençli parazit popülasyonlarının ortaya çıkması, toksik etkiler ve yüksek maliyet nedeniyle tehdit altındadır. Bu nedenle, tedavi edici bitkilerden parazitlere karşı daha etkili ilaçların ortaya çıkarılması, bu sorunun üstesinden gelmek için önemli bir yaklaşım olarak görülmektedir. Çalışmada, Aloe vera (L.) Burm.f. and Hypericum perforatum L. bitki ekstraktlarının Leishmania tropica’nın promastigot formlarına karşı in vitro etkinliğinin değerlendirilmesi amaçlandı. Bitki ekstraktlarının in vitro antileishmanial aktivitesi mikrodilüsyon yöntemi kullanılarak belirlendi. Ekstraktların azalan konsantrasyonları (25 ila 0.01 mg/mL) Leishmania promastigotları üzerinde test edildi. Bitki ekstraktlarının L. tropica promastigotlarının canlılığı üzerindeki etkisi, Neubauer hemositometrede canlı veya hareketli formlar sayılarak değerlendirildi ve veriler kontrollere kıyasla % büyüme olarak değerlendirildi. Her ekstrakt için %50 inhibitör konsantrasyon (IC50) değerleri, Quest Graph™ IC50 Hesaplayıcı kullanılarak lojistik regresyon analizi ile belirlendi. A. vera (L.) Burm.f., L. tropica promastigotları üzerinde %100 büyüme inhibisyonu ile 25, 12.5 ve 6.25 mg/mL’lik yüksek konsantrasyonlarda Leishmania parazitlerini öldürücü aktivite gösterirken, H. perforatum L.’un 25 ila 1.56 mg/mL konsantrasyon aralığında etkili olduğu bulundu. H. perforatum L.’nin IC50’si 0.23 mg/mL ve A. vera (L.) Burm.f’nin IC50’si 1.91 mg/mL olarak belirlendi. Çalışmamız, A. vera (L.) Burm.f. ve H. perforatum L. yapraklarının, leishmaniasis tedavisi için potansiyel tıbbi alternatif olabileceğini gösterdi. Bu bitki ekstraktlarının antiparazitik etkinliği, antileishmanial ajanlarının belirlenmesinde büyük bir gelişme olarak düşünülebilir ve daha ileri in vivo çalışmalarla desteklenmelidir

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